Special considerations for studies of extracellular vesicles from parasitic helminths: A community‐led roadmap to increase rigour and reproducibility

• Autores: White, Ruby; Sotillo, Javier; Ancarola, María Eugenia; Borup, Anne; Boysen, Anders Toftegaard; Brindley, Paul J.; Buzás, Edit I.; Cavallero, Serena; Chaiyadet, Sujittra; Chalmers, Iain W.; Cucher, Marcela Alejandra; Dagenais, Maude; Davis, Chelsea N.; Devaney, Eileen; Duque Correa, Maria A.; Eichenberger, Ramon Marc; Fontenla, Santiago; Gasan, Thomas A.; Hokke, Cornelis H.; Kosanovic, Maja; Kuipers, Marije E.; Laha, Thewarach; Loukas, Alex; Maizels, Rick M.; Marcilla, Antonio; Whitehead, Bradley; Zakeri, Amin; Nejsum, Peter; Buck, Amy H.; Hoffmann, Karl F.
• Año de publicación: 2023
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: electron microscopy; EV guidelines; EV reporting; extracellular vesicles; helminths; parasites
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms. These challenges include, but are not limited to, generating sufficient quantities of EVs for descriptive or functional studies, defining pan-helminth EV markers, genetically modifying these organisms, and identifying rigorous methodologies for in vitro and in vivo studies. Here, we outline best practices for those investigating the biology of helminth-derived EVs to complement the MISEV guidelines. We summarise community-agreed standards for studying EVs derived from this broad set of non-model organisms, raise awareness of issues associated with helminth EVs and provide future perspectives for how progress in the field will be achieved.
Fil: White, Ruby. University of Edinburgh; Reino Unido
Fil: Sotillo, Javier. Instituto de Salud Carlos III; España
Fil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Borup, Anne. University Aarhus; Dinamarca
Fil: Boysen, Anders Toftegaard. University Aarhus; Dinamarca
Fil: Brindley, Paul J.. The George Washington University; Estados Unidos
Fil: Buzás, Edit I.. Immune Proteogenomics Extracellular Vesicle Group; Hungría. Hcemm-su Extracellular Vesicle Research Group; Hungría
Fil: Cavallero, Serena. Università degli Studi di Roma "La Sapienza"; Italia
Fil: Chaiyadet, Sujittra. Khon Kaen Universit; Tailandia
Fil: Chalmers, Iain W.. Aberystwyth University.; Reino Unido
Fil: Cucher, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Dagenais, Maude. McGill University; Canadá
Fil: Davis, Chelsea N.. Aberystwyth University.; Reino Unido
Fil: Devaney, Eileen. University of Glasgow; Reino Unido
Fil: Duque Correa, Maria A.. Wellcome Sanger Institute; Reino Unido
Fil: Eichenberger, Ramon Marc. Universitat Zurich; Suiza
Fil: Fontenla, Santiago. Universidad de la Republica. Facultad de Medicina; Uruguay
Fil: Gasan, Thomas A.. Queen’s University Belfast; Reino Unido
Fil: Hokke, Cornelis H.. University of Utrecht; Países Bajos
Fil: Kosanovic, Maja. University of Belgrade; Serbia
Fil: Kuipers, Marije E.. Leiden University; Países Bajos. University of Utrecht; Países Bajos
Fil: Laha, Thewarach. Khon Kaen University; Tailandia
Fil: Loukas, Alex. James Cook University; Australia
Fil: Maizels, Rick M.. University of Glasgow; Reino Unido
Fil: Marcilla, Antonio. Dpto Farmacia y Tecnologia Farmaceutica y Parasitologia; España
Fil: Whitehead, Bradley. Universidad de Valencia; España
Fil: Zakeri, Amin. University Aarhus; Dinamarca
Fil: Nejsum, Peter. University Aarhus; Dinamarca
Fil: Buck, Amy H.. University of Edinburgh; Reino Unido
Fil: Hoffmann, Karl F.. Aberystwyth University.; Reino Unido