Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone

Autores
Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernández, Héctor; Zon, María Alicia; Molina, Patricia Gabriela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Integrated immunosensors are based on the spatial integration of the sensing layer and the detection system. In this study, we report an integrated immunosensor to determine progesterone (P4) in bovine serum samples. P4 is a steroidal hormone with a vital role in the maintenance of human and animal health. Therefore, an integrated immunosensor for the determination of P4 based on direct attachment of anti-progesterone monoclonal antibody (mAbP4) on a modified gold disk (Au disk) electrode with gold nanoparticles (Au NP) lodged on a cysteamine (cys) self-assembled monolayer (mAbP4-Au NP-cys-Au disk) is reported. The immunosensor is based on a competitive assay involving P4 labeled with horseradish peroxidase (HRP), which oxidizes pyrocatechol (H 2Q), in the presence of hydrogen peroxide (H 2O 2), to benzoquinone (Q). Its back electrochemical reduction to H 2Q is detected on the surface of mAbP4-Au NP-cys-Au disk electrode. Different experimental variables involved in the immunosensor preparation such as the gold nanoparticles loading, the amount of mAbP4 immobilized, and the cysteamine immobilization time were optimized. The calibration curve showed an IC 50 of 0.54 ng mL -1, and a detection limit (DL) of 0.08 ng mL -1, with a linear range from 8 × 10 -2 to 7 ng mL -1. Good percent relative standard deviations (% RSD) values were obtained for bovine serum samples containing 1 and 10 ng mL -1, i.e., about 6%. The recovery values were 109.6 and 110.1% for 1 and 10 ng mL -1, respectively. The assays are fast, selective and very sensitive. Thus, the immunosensor shows to be a very useful tool to determine P4 in bovine serum samples without any pretreatment.
Fil: Monerris, Melisa Jimena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
ENZYME IMMUNOASSAYS
GOLD NANOPARTICLES
IMMUNOSENSOR
PROGESTERONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189196
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189196
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesteroneMonerris, Melisa JimenaArevalo, Fernando JavierFernández, HéctorZon, María AliciaMolina, Patricia GabrielaENZYME IMMUNOASSAYSGOLD NANOPARTICLESIMMUNOSENSORPROGESTERONEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Integrated immunosensors are based on the spatial integration of the sensing layer and the detection system. In this study, we report an integrated immunosensor to determine progesterone (P4) in bovine serum samples. P4 is a steroidal hormone with a vital role in the maintenance of human and animal health. Therefore, an integrated immunosensor for the determination of P4 based on direct attachment of anti-progesterone monoclonal antibody (mAbP4) on a modified gold disk (Au disk) electrode with gold nanoparticles (Au NP) lodged on a cysteamine (cys) self-assembled monolayer (mAbP4-Au NP-cys-Au disk) is reported. The immunosensor is based on a competitive assay involving P4 labeled with horseradish peroxidase (HRP), which oxidizes pyrocatechol (H 2Q), in the presence of hydrogen peroxide (H 2O 2), to benzoquinone (Q). Its back electrochemical reduction to H 2Q is detected on the surface of mAbP4-Au NP-cys-Au disk electrode. Different experimental variables involved in the immunosensor preparation such as the gold nanoparticles loading, the amount of mAbP4 immobilized, and the cysteamine immobilization time were optimized. The calibration curve showed an IC 50 of 0.54 ng mL -1, and a detection limit (DL) of 0.08 ng mL -1, with a linear range from 8 × 10 -2 to 7 ng mL -1. Good percent relative standard deviations (% RSD) values were obtained for bovine serum samples containing 1 and 10 ng mL -1, i.e., about 6%. The recovery values were 109.6 and 110.1% for 1 and 10 ng mL -1, respectively. The assays are fast, selective and very sensitive. Thus, the immunosensor shows to be a very useful tool to determine P4 in bovine serum samples without any pretreatment.Fil: Monerris, Melisa Jimena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaElsevier Science SA2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189196Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernández, Héctor; Zon, María Alicia; Molina, Patricia Gabriela; Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone; Elsevier Science SA; Sensors and Actuators B: Chemical; 166-167; 3-2012; 586-5920925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400512002663info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2012.03.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:03Zoai:ri.conicet.gov.ar:11336/189196instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:03.974CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
title Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
spellingShingle Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
Monerris, Melisa Jimena
ENZYME IMMUNOASSAYS
GOLD NANOPARTICLES
IMMUNOSENSOR
PROGESTERONE
title_short Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
title_full Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
title_fullStr Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
title_full_unstemmed Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
title_sort Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone
dc.creator.none.fl_str_mv Monerris, Melisa Jimena
Arevalo, Fernando Javier
Fernández, Héctor
Zon, María Alicia
Molina, Patricia Gabriela
author Monerris, Melisa Jimena
author_facet Monerris, Melisa Jimena
Arevalo, Fernando Javier
Fernández, Héctor
Zon, María Alicia
Molina, Patricia Gabriela
author_role author
author2 Arevalo, Fernando Javier
Fernández, Héctor
Zon, María Alicia
Molina, Patricia Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENZYME IMMUNOASSAYS
GOLD NANOPARTICLES
IMMUNOSENSOR
PROGESTERONE
topic ENZYME IMMUNOASSAYS
GOLD NANOPARTICLES
IMMUNOSENSOR
PROGESTERONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Integrated immunosensors are based on the spatial integration of the sensing layer and the detection system. In this study, we report an integrated immunosensor to determine progesterone (P4) in bovine serum samples. P4 is a steroidal hormone with a vital role in the maintenance of human and animal health. Therefore, an integrated immunosensor for the determination of P4 based on direct attachment of anti-progesterone monoclonal antibody (mAbP4) on a modified gold disk (Au disk) electrode with gold nanoparticles (Au NP) lodged on a cysteamine (cys) self-assembled monolayer (mAbP4-Au NP-cys-Au disk) is reported. The immunosensor is based on a competitive assay involving P4 labeled with horseradish peroxidase (HRP), which oxidizes pyrocatechol (H 2Q), in the presence of hydrogen peroxide (H 2O 2), to benzoquinone (Q). Its back electrochemical reduction to H 2Q is detected on the surface of mAbP4-Au NP-cys-Au disk electrode. Different experimental variables involved in the immunosensor preparation such as the gold nanoparticles loading, the amount of mAbP4 immobilized, and the cysteamine immobilization time were optimized. The calibration curve showed an IC 50 of 0.54 ng mL -1, and a detection limit (DL) of 0.08 ng mL -1, with a linear range from 8 × 10 -2 to 7 ng mL -1. Good percent relative standard deviations (% RSD) values were obtained for bovine serum samples containing 1 and 10 ng mL -1, i.e., about 6%. The recovery values were 109.6 and 110.1% for 1 and 10 ng mL -1, respectively. The assays are fast, selective and very sensitive. Thus, the immunosensor shows to be a very useful tool to determine P4 in bovine serum samples without any pretreatment.
Fil: Monerris, Melisa Jimena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Integrated immunosensors are based on the spatial integration of the sensing layer and the detection system. In this study, we report an integrated immunosensor to determine progesterone (P4) in bovine serum samples. P4 is a steroidal hormone with a vital role in the maintenance of human and animal health. Therefore, an integrated immunosensor for the determination of P4 based on direct attachment of anti-progesterone monoclonal antibody (mAbP4) on a modified gold disk (Au disk) electrode with gold nanoparticles (Au NP) lodged on a cysteamine (cys) self-assembled monolayer (mAbP4-Au NP-cys-Au disk) is reported. The immunosensor is based on a competitive assay involving P4 labeled with horseradish peroxidase (HRP), which oxidizes pyrocatechol (H 2Q), in the presence of hydrogen peroxide (H 2O 2), to benzoquinone (Q). Its back electrochemical reduction to H 2Q is detected on the surface of mAbP4-Au NP-cys-Au disk electrode. Different experimental variables involved in the immunosensor preparation such as the gold nanoparticles loading, the amount of mAbP4 immobilized, and the cysteamine immobilization time were optimized. The calibration curve showed an IC 50 of 0.54 ng mL -1, and a detection limit (DL) of 0.08 ng mL -1, with a linear range from 8 × 10 -2 to 7 ng mL -1. Good percent relative standard deviations (% RSD) values were obtained for bovine serum samples containing 1 and 10 ng mL -1, i.e., about 6%. The recovery values were 109.6 and 110.1% for 1 and 10 ng mL -1, respectively. The assays are fast, selective and very sensitive. Thus, the immunosensor shows to be a very useful tool to determine P4 in bovine serum samples without any pretreatment.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189196
Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernández, Héctor; Zon, María Alicia; Molina, Patricia Gabriela; Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone; Elsevier Science SA; Sensors and Actuators B: Chemical; 166-167; 3-2012; 586-592
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189196
identifier_str_mv Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernández, Héctor; Zon, María Alicia; Molina, Patricia Gabriela; Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone; Elsevier Science SA; Sensors and Actuators B: Chemical; 166-167; 3-2012; 586-592
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400512002663
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2012.03.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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