Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis

Autores
Podio, Maricel; Cáceres, María Emilia; Samoluk, Sergio Sebastián; Seijo, Jose Guillermo; Pessino, Silvina Claudia; Ortiz, Juan Pablo Amelio; Pupilli, Fulvio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single locus which is blocked in terms of recombination. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin, namely the presence of repetitive elements, gene degeneration, and de-regulation. To test the epigenetic control of apomixis, a study on the distribution of cytosine methylation at the apomixis locus and the effect of artificial DNA demethylation on the mode of reproduction was undertaken in two apomictic Paspalum species. The 5-methylcytosine distribution in the apomixis-controlling genomic region was studied in P. simplex by methylation-sensitive restriction fragment length polymorphism (RFLP) analysis and in P. notatum by fluorescene in situ hybridization (FISH). The effect of DNA demethylation was studied on the mode of reproduction of P. simplex by progeny test analysis of apomictic plants treated with the demethylating agent 5'-azacytidine. A high level of cytosine methylation was detected at the apomixis-controlling genomic region in both species. By analysing a total of 374 open pollination progeny, it was found that artificial demethylation had little or no effect on apospory, whereas it induced a significant depression of parthenogenesis. The results suggested that factors controlling repression of parthenogenesis might be inactivated in apomictic Paspalum by DNA methylation.
Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina
Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique; Francia
Fil: Samoluk, Sergio Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pupilli, Fulvio. Centre National de la Recherche Scientifique; Francia
Materia
5'-AZACYTIDINE
APOMIXIS
BIII HYBRIDS
DNA METHYLATION
EPIGENETICS
PARTHENOGENESIS.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2733
Acceder
id CONICETDig_d3e06f6c5495cce4b2fe963dc3c04557
oai_identifier_str oai:ri.conicet.gov.ar:11336/2733
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesisPodio, MaricelCáceres, María EmiliaSamoluk, Sergio SebastiánSeijo, Jose GuillermoPessino, Silvina ClaudiaOrtiz, Juan Pablo AmelioPupilli, Fulvio5'-AZACYTIDINEAPOMIXISBIII HYBRIDSDNA METHYLATIONEPIGENETICSPARTHENOGENESIS.https://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single locus which is blocked in terms of recombination. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin, namely the presence of repetitive elements, gene degeneration, and de-regulation. To test the epigenetic control of apomixis, a study on the distribution of cytosine methylation at the apomixis locus and the effect of artificial DNA demethylation on the mode of reproduction was undertaken in two apomictic Paspalum species. The 5-methylcytosine distribution in the apomixis-controlling genomic region was studied in P. simplex by methylation-sensitive restriction fragment length polymorphism (RFLP) analysis and in P. notatum by fluorescene in situ hybridization (FISH). The effect of DNA demethylation was studied on the mode of reproduction of P. simplex by progeny test analysis of apomictic plants treated with the demethylating agent 5'-azacytidine. A high level of cytosine methylation was detected at the apomixis-controlling genomic region in both species. By analysing a total of 374 open pollination progeny, it was found that artificial demethylation had little or no effect on apospory, whereas it induced a significant depression of parthenogenesis. The results suggested that factors controlling repression of parthenogenesis might be inactivated in apomictic Paspalum by DNA methylation.Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; ArgentinaFil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique; FranciaFil: Samoluk, Sergio Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; ArgentinaFil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Pupilli, Fulvio. Centre National de la Recherche Scientifique; FranciaOxford University Press2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2733Podio, Maricel; Cáceres, María Emilia; Samoluk, Sergio Sebastián; Seijo, Jose Guillermo; Pessino, Silvina Claudia; et al.; Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis; Oxford University Press; Journal of Experimental Botany; 65; 22; 12-2014; 6411-64240022-0957enginfo:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/65/22/6411.longinfo:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eru354info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:03Zoai:ri.conicet.gov.ar:11336/2733instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:03.944CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
title Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
spellingShingle Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
Podio, Maricel
5'-AZACYTIDINE
APOMIXIS
BIII HYBRIDS
DNA METHYLATION
EPIGENETICS
PARTHENOGENESIS.
title_short Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
title_full Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
title_fullStr Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
title_full_unstemmed Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
title_sort Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis
dc.creator.none.fl_str_mv Podio, Maricel
Cáceres, María Emilia
Samoluk, Sergio Sebastián
Seijo, Jose Guillermo
Pessino, Silvina Claudia
Ortiz, Juan Pablo Amelio
Pupilli, Fulvio
author Podio, Maricel
author_facet Podio, Maricel
Cáceres, María Emilia
Samoluk, Sergio Sebastián
Seijo, Jose Guillermo
Pessino, Silvina Claudia
Ortiz, Juan Pablo Amelio
Pupilli, Fulvio
author_role author
author2 Cáceres, María Emilia
Samoluk, Sergio Sebastián
Seijo, Jose Guillermo
Pessino, Silvina Claudia
Ortiz, Juan Pablo Amelio
Pupilli, Fulvio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv 5'-AZACYTIDINE
APOMIXIS
BIII HYBRIDS
DNA METHYLATION
EPIGENETICS
PARTHENOGENESIS.
topic 5'-AZACYTIDINE
APOMIXIS
BIII HYBRIDS
DNA METHYLATION
EPIGENETICS
PARTHENOGENESIS.
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single locus which is blocked in terms of recombination. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin, namely the presence of repetitive elements, gene degeneration, and de-regulation. To test the epigenetic control of apomixis, a study on the distribution of cytosine methylation at the apomixis locus and the effect of artificial DNA demethylation on the mode of reproduction was undertaken in two apomictic Paspalum species. The 5-methylcytosine distribution in the apomixis-controlling genomic region was studied in P. simplex by methylation-sensitive restriction fragment length polymorphism (RFLP) analysis and in P. notatum by fluorescene in situ hybridization (FISH). The effect of DNA demethylation was studied on the mode of reproduction of P. simplex by progeny test analysis of apomictic plants treated with the demethylating agent 5'-azacytidine. A high level of cytosine methylation was detected at the apomixis-controlling genomic region in both species. By analysing a total of 374 open pollination progeny, it was found that artificial demethylation had little or no effect on apospory, whereas it induced a significant depression of parthenogenesis. The results suggested that factors controlling repression of parthenogenesis might be inactivated in apomictic Paspalum by DNA methylation.
Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina
Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique; Francia
Fil: Samoluk, Sergio Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pupilli, Fulvio. Centre National de la Recherche Scientifique; Francia
description Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single locus which is blocked in terms of recombination. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin, namely the presence of repetitive elements, gene degeneration, and de-regulation. To test the epigenetic control of apomixis, a study on the distribution of cytosine methylation at the apomixis locus and the effect of artificial DNA demethylation on the mode of reproduction was undertaken in two apomictic Paspalum species. The 5-methylcytosine distribution in the apomixis-controlling genomic region was studied in P. simplex by methylation-sensitive restriction fragment length polymorphism (RFLP) analysis and in P. notatum by fluorescene in situ hybridization (FISH). The effect of DNA demethylation was studied on the mode of reproduction of P. simplex by progeny test analysis of apomictic plants treated with the demethylating agent 5'-azacytidine. A high level of cytosine methylation was detected at the apomixis-controlling genomic region in both species. By analysing a total of 374 open pollination progeny, it was found that artificial demethylation had little or no effect on apospory, whereas it induced a significant depression of parthenogenesis. The results suggested that factors controlling repression of parthenogenesis might be inactivated in apomictic Paspalum by DNA methylation.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2733
Podio, Maricel; Cáceres, María Emilia; Samoluk, Sergio Sebastián; Seijo, Jose Guillermo; Pessino, Silvina Claudia; et al.; Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis; Oxford University Press; Journal of Experimental Botany; 65; 22; 12-2014; 6411-6424
0022-0957
url http://hdl.handle.net/11336/2733
identifier_str_mv Podio, Maricel; Cáceres, María Emilia; Samoluk, Sergio Sebastián; Seijo, Jose Guillermo; Pessino, Silvina Claudia; et al.; Analysis of the methylation status of the apomixis-specific region in Paspalum spp. suggests an epigenetic control on parthenogenesis; Oxford University Press; Journal of Experimental Botany; 65; 22; 12-2014; 6411-6424
0022-0957
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/65/22/6411.long
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eru354
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269834644029440
score 13.13397

Publicaciones similares