Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.

Autores
Adler, Conrado; Michavila, Gabriela; Lopez, Fabian Enrique; Corbalan, Natalia Soledad; Lami, María Jesús; Filippone, María Paula; Castagnaro, Atilio Pedro; Vincent, Paula Andrea; de Cristobal, Ricardo Ezequiel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work shows the identification of a compound able to inhibit Xanthomonas citri subsp. citri (citrus canker agent) growth in vitro and in vivo. Firstly, we isolated from citrus leaves surfaces, bacteria able to inhibit X. citri subsp. citri in vitro. Among the selected isolates, we focused in one showing a remarkable activity. The strain was characterized as Pseudomonas fluorescens after sequencing its 16S rDNA and analyzing the sequence with BLASTn. Purification and chemical analysis of the active compound allowed us to assign the inhibitory activity to enantio-pyochelin (E-Pch). Since this molecule is a siderophore, we wondered if the inhibition observed was a result of iron scavenging. Surprisingly, when we supplemented media with an excess of iron, we observed practically no change in the inhibition activity. In an attempt to identify the action mechanism of E-Pch, we evaluated the ability of E-Pch to generate reactive oxygen species (ROS) within a culture of X. citri subsp. citri and its correlation with the inhibitory activity. In fact, we observed increased ROS levels when E-Pch was added. In addition, the reducer agent ascorbic acid, lowered ROS levels and the antibiotic activity, implying that inhibition is probably caused by oxidative stress. Finally, we studied the use of E-Pch in a model of canker disease. E-Pch showed to reduce canker formation on leaves of Eureka and Lisbon lemon cultivars. These results show E-Pch as a promising compound for citrus canker biocontrol.
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Michavila, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lopez, Fabian Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lami, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Filippone, María Paula. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Castagnaro, Atilio Pedro. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Biocontrol
Xanthomonas
Antagonism
Reactive Oxygen Species
Lemon
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47488
Acceder
id CONICETDig_d3d1fe7302bfe75451def490144ba991
oai_identifier_str oai:ri.conicet.gov.ar:11336/47488
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.Adler, ConradoMichavila, GabrielaLopez, Fabian EnriqueCorbalan, Natalia SoledadLami, María JesúsFilippone, María PaulaCastagnaro, Atilio PedroVincent, Paula Andreade Cristobal, Ricardo EzequielBiocontrolXanthomonasAntagonismReactive Oxygen SpeciesLemonhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This work shows the identification of a compound able to inhibit Xanthomonas citri subsp. citri (citrus canker agent) growth in vitro and in vivo. Firstly, we isolated from citrus leaves surfaces, bacteria able to inhibit X. citri subsp. citri in vitro. Among the selected isolates, we focused in one showing a remarkable activity. The strain was characterized as Pseudomonas fluorescens after sequencing its 16S rDNA and analyzing the sequence with BLASTn. Purification and chemical analysis of the active compound allowed us to assign the inhibitory activity to enantio-pyochelin (E-Pch). Since this molecule is a siderophore, we wondered if the inhibition observed was a result of iron scavenging. Surprisingly, when we supplemented media with an excess of iron, we observed practically no change in the inhibition activity. In an attempt to identify the action mechanism of E-Pch, we evaluated the ability of E-Pch to generate reactive oxygen species (ROS) within a culture of X. citri subsp. citri and its correlation with the inhibitory activity. In fact, we observed increased ROS levels when E-Pch was added. In addition, the reducer agent ascorbic acid, lowered ROS levels and the antibiotic activity, implying that inhibition is probably caused by oxidative stress. Finally, we studied the use of E-Pch in a model of canker disease. E-Pch showed to reduce canker formation on leaves of Eureka and Lisbon lemon cultivars. These results show E-Pch as a promising compound for citrus canker biocontrol.Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Michavila, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Lopez, Fabian Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Lami, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Filippone, María Paula. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Castagnaro, Atilio Pedro. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaInternational Society for Horticultural Science2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47488Adler, Conrado; Michavila, Gabriela; Lopez, Fabian Enrique; Corbalan, Natalia Soledad; Lami, María Jesús; et al.; Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.; International Society for Horticultural Science; Acta Horticulturae; 1065; 1; 1-2015; 937-9450567-7572CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.actahort.org/books/1065/1065_117.htminfo:eu-repo/semantics/altIdentifier/url/https://doi.org/10.17660/ActaHortic.2015.1065.117info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:47:18Zoai:ri.conicet.gov.ar:11336/47488instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:47:19.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
title Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
spellingShingle Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
Adler, Conrado
Biocontrol
Xanthomonas
Antagonism
Reactive Oxygen Species
Lemon
title_short Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
title_full Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
title_fullStr Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
title_full_unstemmed Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
title_sort Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.
dc.creator.none.fl_str_mv Adler, Conrado
Michavila, Gabriela
Lopez, Fabian Enrique
Corbalan, Natalia Soledad
Lami, María Jesús
Filippone, María Paula
Castagnaro, Atilio Pedro
Vincent, Paula Andrea
de Cristobal, Ricardo Ezequiel
author Adler, Conrado
author_facet Adler, Conrado
Michavila, Gabriela
Lopez, Fabian Enrique
Corbalan, Natalia Soledad
Lami, María Jesús
Filippone, María Paula
Castagnaro, Atilio Pedro
Vincent, Paula Andrea
de Cristobal, Ricardo Ezequiel
author_role author
author2 Michavila, Gabriela
Lopez, Fabian Enrique
Corbalan, Natalia Soledad
Lami, María Jesús
Filippone, María Paula
Castagnaro, Atilio Pedro
Vincent, Paula Andrea
de Cristobal, Ricardo Ezequiel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biocontrol
Xanthomonas
Antagonism
Reactive Oxygen Species
Lemon
topic Biocontrol
Xanthomonas
Antagonism
Reactive Oxygen Species
Lemon
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work shows the identification of a compound able to inhibit Xanthomonas citri subsp. citri (citrus canker agent) growth in vitro and in vivo. Firstly, we isolated from citrus leaves surfaces, bacteria able to inhibit X. citri subsp. citri in vitro. Among the selected isolates, we focused in one showing a remarkable activity. The strain was characterized as Pseudomonas fluorescens after sequencing its 16S rDNA and analyzing the sequence with BLASTn. Purification and chemical analysis of the active compound allowed us to assign the inhibitory activity to enantio-pyochelin (E-Pch). Since this molecule is a siderophore, we wondered if the inhibition observed was a result of iron scavenging. Surprisingly, when we supplemented media with an excess of iron, we observed practically no change in the inhibition activity. In an attempt to identify the action mechanism of E-Pch, we evaluated the ability of E-Pch to generate reactive oxygen species (ROS) within a culture of X. citri subsp. citri and its correlation with the inhibitory activity. In fact, we observed increased ROS levels when E-Pch was added. In addition, the reducer agent ascorbic acid, lowered ROS levels and the antibiotic activity, implying that inhibition is probably caused by oxidative stress. Finally, we studied the use of E-Pch in a model of canker disease. E-Pch showed to reduce canker formation on leaves of Eureka and Lisbon lemon cultivars. These results show E-Pch as a promising compound for citrus canker biocontrol.
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Michavila, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lopez, Fabian Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lami, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Filippone, María Paula. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Castagnaro, Atilio Pedro. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description This work shows the identification of a compound able to inhibit Xanthomonas citri subsp. citri (citrus canker agent) growth in vitro and in vivo. Firstly, we isolated from citrus leaves surfaces, bacteria able to inhibit X. citri subsp. citri in vitro. Among the selected isolates, we focused in one showing a remarkable activity. The strain was characterized as Pseudomonas fluorescens after sequencing its 16S rDNA and analyzing the sequence with BLASTn. Purification and chemical analysis of the active compound allowed us to assign the inhibitory activity to enantio-pyochelin (E-Pch). Since this molecule is a siderophore, we wondered if the inhibition observed was a result of iron scavenging. Surprisingly, when we supplemented media with an excess of iron, we observed practically no change in the inhibition activity. In an attempt to identify the action mechanism of E-Pch, we evaluated the ability of E-Pch to generate reactive oxygen species (ROS) within a culture of X. citri subsp. citri and its correlation with the inhibitory activity. In fact, we observed increased ROS levels when E-Pch was added. In addition, the reducer agent ascorbic acid, lowered ROS levels and the antibiotic activity, implying that inhibition is probably caused by oxidative stress. Finally, we studied the use of E-Pch in a model of canker disease. E-Pch showed to reduce canker formation on leaves of Eureka and Lisbon lemon cultivars. These results show E-Pch as a promising compound for citrus canker biocontrol.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47488
Adler, Conrado; Michavila, Gabriela; Lopez, Fabian Enrique; Corbalan, Natalia Soledad; Lami, María Jesús; et al.; Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.; International Society for Horticultural Science; Acta Horticulturae; 1065; 1; 1-2015; 937-945
0567-7572
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47488
identifier_str_mv Adler, Conrado; Michavila, Gabriela; Lopez, Fabian Enrique; Corbalan, Natalia Soledad; Lami, María Jesús; et al.; Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens.; International Society for Horticultural Science; Acta Horticulturae; 1065; 1; 1-2015; 937-945
0567-7572
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.actahort.org/books/1065/1065_117.htm
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.17660/ActaHortic.2015.1065.117
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Society for Horticultural Science
publisher.none.fl_str_mv International Society for Horticultural Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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