Towards the sustainable production of lemons: native yeasts as biological control agents

Autores
Diaz, Mariana Andrea; Pereyra, Martina María; Rasuk, Maria Cecilia; Dib, Julian Rafael
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The province of Tucumán is positioned as one of the main centers of production and processing of lemons. Among the problems associated with the production of lemons, the economic losses caused by postharvest fungal infections stand out. Chemical fungicides have traditionally been applied as control and preventive measures, but their use entails numerous problems such as environmental contamination, toxicity in humans and restrictions on international markets. However, efficient and sustainable alternatives to the use of such pesticides are not known so far. Thus, the aim of this research was to search for biological control alternatives based on native yeasts, understand the associated mechanisms of action, and formulate a sustainable biocontrol agent. Biocontrol yeasts were isolated from citrus plants and lemon packinghouses. The antagonism of the isolates against Penicillium digitatum in in vitro and in vivo assays as well as the possible mechanisms of action related to biological control were determined. In addition, both liquid and solid formulations were tested. Among the yeast isolates, Clavispora lusitaniae 146stood out as it was able to inhibit P. digitatum in vitro and also to control green mold onlemons with high efficiency, both in room temperature and cold storage. Yeast 146 was ableto resist stress factors associated with the packaging process and was compatible with carnauba wax. Among the mechanisms of action, the ability to colonize wounds and the competition for space and nutrients were evidenced. C. lusitaniae depicted a broad spectrum of action in controlling green mold on other citrus fruits, such as oranges, mandarins, and grapefruit. C. lusitaniae 146 did not negatively affect the aroma perception of fruits by consumers. Moreover, it was able to remove the mycotoxin patulin from a solution. Different liquid and solid formulations were tested; being the yeast dried by lyophilization in combination with sucrose and skimmed milk the most promising. The native yeast C. lusitaniae 146 is a promising biological control agent for the control of P. digitatum in lemons and other varieties of citrus fruits. A yeast-based formulation would replace the use of chemical fungicides and promote the organic production of lemons.
Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
XVIII Congreso de la Sociedad Argentina de Microbiología General
Chapadmalal
Argentina
Sociedad Argentina de Microbiología General
Materia
LEMON
YEAST
BIOCONTROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217236
Acceder
id CONICETDig_031ec76973416f6252543007280c30de
oai_identifier_str oai:ri.conicet.gov.ar:11336/217236
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Towards the sustainable production of lemons: native yeasts as biological control agentsDiaz, Mariana AndreaPereyra, Martina MaríaRasuk, Maria CeciliaDib, Julian RafaelLEMONYEASTBIOCONTROLhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The province of Tucumán is positioned as one of the main centers of production and processing of lemons. Among the problems associated with the production of lemons, the economic losses caused by postharvest fungal infections stand out. Chemical fungicides have traditionally been applied as control and preventive measures, but their use entails numerous problems such as environmental contamination, toxicity in humans and restrictions on international markets. However, efficient and sustainable alternatives to the use of such pesticides are not known so far. Thus, the aim of this research was to search for biological control alternatives based on native yeasts, understand the associated mechanisms of action, and formulate a sustainable biocontrol agent. Biocontrol yeasts were isolated from citrus plants and lemon packinghouses. The antagonism of the isolates against Penicillium digitatum in in vitro and in vivo assays as well as the possible mechanisms of action related to biological control were determined. In addition, both liquid and solid formulations were tested. Among the yeast isolates, Clavispora lusitaniae 146stood out as it was able to inhibit P. digitatum in vitro and also to control green mold onlemons with high efficiency, both in room temperature and cold storage. Yeast 146 was ableto resist stress factors associated with the packaging process and was compatible with carnauba wax. Among the mechanisms of action, the ability to colonize wounds and the competition for space and nutrients were evidenced. C. lusitaniae depicted a broad spectrum of action in controlling green mold on other citrus fruits, such as oranges, mandarins, and grapefruit. C. lusitaniae 146 did not negatively affect the aroma perception of fruits by consumers. Moreover, it was able to remove the mycotoxin patulin from a solution. Different liquid and solid formulations were tested; being the yeast dried by lyophilization in combination with sucrose and skimmed milk the most promising. The native yeast C. lusitaniae 146 is a promising biological control agent for the control of P. digitatum in lemons and other varieties of citrus fruits. A yeast-based formulation would replace the use of chemical fungicides and promote the organic production of lemons.Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaXVIII Congreso de la Sociedad Argentina de Microbiología GeneralChapadmalalArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217236Towards the sustainable production of lemons: native yeasts as biological control agents; XVIII Congreso de la Sociedad Argentina de Microbiología General; Chapadmalal; Argentina; 2023; 30-30978-631-90156-1-4CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2023/09/Libro-de-Resumenes-Final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:32Zoai:ri.conicet.gov.ar:11336/217236instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:32.829CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Towards the sustainable production of lemons: native yeasts as biological control agents
title Towards the sustainable production of lemons: native yeasts as biological control agents
spellingShingle Towards the sustainable production of lemons: native yeasts as biological control agents
Diaz, Mariana Andrea
LEMON
YEAST
BIOCONTROL
title_short Towards the sustainable production of lemons: native yeasts as biological control agents
title_full Towards the sustainable production of lemons: native yeasts as biological control agents
title_fullStr Towards the sustainable production of lemons: native yeasts as biological control agents
title_full_unstemmed Towards the sustainable production of lemons: native yeasts as biological control agents
title_sort Towards the sustainable production of lemons: native yeasts as biological control agents
dc.creator.none.fl_str_mv Diaz, Mariana Andrea
Pereyra, Martina María
Rasuk, Maria Cecilia
Dib, Julian Rafael
author Diaz, Mariana Andrea
author_facet Diaz, Mariana Andrea
Pereyra, Martina María
Rasuk, Maria Cecilia
Dib, Julian Rafael
author_role author
author2 Pereyra, Martina María
Rasuk, Maria Cecilia
Dib, Julian Rafael
author2_role author
author
author
dc.subject.none.fl_str_mv LEMON
YEAST
BIOCONTROL
topic LEMON
YEAST
BIOCONTROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The province of Tucumán is positioned as one of the main centers of production and processing of lemons. Among the problems associated with the production of lemons, the economic losses caused by postharvest fungal infections stand out. Chemical fungicides have traditionally been applied as control and preventive measures, but their use entails numerous problems such as environmental contamination, toxicity in humans and restrictions on international markets. However, efficient and sustainable alternatives to the use of such pesticides are not known so far. Thus, the aim of this research was to search for biological control alternatives based on native yeasts, understand the associated mechanisms of action, and formulate a sustainable biocontrol agent. Biocontrol yeasts were isolated from citrus plants and lemon packinghouses. The antagonism of the isolates against Penicillium digitatum in in vitro and in vivo assays as well as the possible mechanisms of action related to biological control were determined. In addition, both liquid and solid formulations were tested. Among the yeast isolates, Clavispora lusitaniae 146stood out as it was able to inhibit P. digitatum in vitro and also to control green mold onlemons with high efficiency, both in room temperature and cold storage. Yeast 146 was ableto resist stress factors associated with the packaging process and was compatible with carnauba wax. Among the mechanisms of action, the ability to colonize wounds and the competition for space and nutrients were evidenced. C. lusitaniae depicted a broad spectrum of action in controlling green mold on other citrus fruits, such as oranges, mandarins, and grapefruit. C. lusitaniae 146 did not negatively affect the aroma perception of fruits by consumers. Moreover, it was able to remove the mycotoxin patulin from a solution. Different liquid and solid formulations were tested; being the yeast dried by lyophilization in combination with sucrose and skimmed milk the most promising. The native yeast C. lusitaniae 146 is a promising biological control agent for the control of P. digitatum in lemons and other varieties of citrus fruits. A yeast-based formulation would replace the use of chemical fungicides and promote the organic production of lemons.
Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
XVIII Congreso de la Sociedad Argentina de Microbiología General
Chapadmalal
Argentina
Sociedad Argentina de Microbiología General
description The province of Tucumán is positioned as one of the main centers of production and processing of lemons. Among the problems associated with the production of lemons, the economic losses caused by postharvest fungal infections stand out. Chemical fungicides have traditionally been applied as control and preventive measures, but their use entails numerous problems such as environmental contamination, toxicity in humans and restrictions on international markets. However, efficient and sustainable alternatives to the use of such pesticides are not known so far. Thus, the aim of this research was to search for biological control alternatives based on native yeasts, understand the associated mechanisms of action, and formulate a sustainable biocontrol agent. Biocontrol yeasts were isolated from citrus plants and lemon packinghouses. The antagonism of the isolates against Penicillium digitatum in in vitro and in vivo assays as well as the possible mechanisms of action related to biological control were determined. In addition, both liquid and solid formulations were tested. Among the yeast isolates, Clavispora lusitaniae 146stood out as it was able to inhibit P. digitatum in vitro and also to control green mold onlemons with high efficiency, both in room temperature and cold storage. Yeast 146 was ableto resist stress factors associated with the packaging process and was compatible with carnauba wax. Among the mechanisms of action, the ability to colonize wounds and the competition for space and nutrients were evidenced. C. lusitaniae depicted a broad spectrum of action in controlling green mold on other citrus fruits, such as oranges, mandarins, and grapefruit. C. lusitaniae 146 did not negatively affect the aroma perception of fruits by consumers. Moreover, it was able to remove the mycotoxin patulin from a solution. Different liquid and solid formulations were tested; being the yeast dried by lyophilization in combination with sucrose and skimmed milk the most promising. The native yeast C. lusitaniae 146 is a promising biological control agent for the control of P. digitatum in lemons and other varieties of citrus fruits. A yeast-based formulation would replace the use of chemical fungicides and promote the organic production of lemons.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217236
Towards the sustainable production of lemons: native yeasts as biological control agents; XVIII Congreso de la Sociedad Argentina de Microbiología General; Chapadmalal; Argentina; 2023; 30-30
978-631-90156-1-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217236
identifier_str_mv Towards the sustainable production of lemons: native yeasts as biological control agents; XVIII Congreso de la Sociedad Argentina de Microbiología General; Chapadmalal; Argentina; 2023; 30-30
978-631-90156-1-4
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2023/09/Libro-de-Resumenes-Final.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269805823918080
score 13.13397

Publicaciones similares