Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome
- Autores
- Rasuk, Maria Cecilia; Pisa, José Horacio; Chacón, Florencia Isabel; Pereyra, Martina María; Dib, Julian Rafael
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The use of biocontrol agents has been proposed as an effective alternative to reduce citrus decays for promoting sustainable agriculture based on organic fruit production. Among the different microbial biocontrol agents, Clavispora lusitaniae 146 stands out as it is able of effectively controlling green mold in lemons. Although there is growing recognition of the role that the microbiome plays in the health and physiology of many plant species, to date, the composition of the lemon microbiome is unknown, nor is the effect of yeast 146 on it. Thus, the aim of this research was to study the impacts of biocontrol yeast Clavispora lusitaneae 146 on the composition of the lemon microbiome. Lemons were harvested, and then divided into two treatments: untreated and treated lemons with biocontrol yeast C. lusitaneae 146. Fruits were then stored at room temperature for 7 days. DNA was extracted from a pool of 3 pieces of peel per sample, and used for PCR that amplified the bacterial hypervariable V3-V4 region of the 16S rRNA gene. Paired-end sequencing of amplicons was done on an Illumina MiSeq sequencer. To assess the effects of postharvest treatment and storage on the diversity of the lemon microbiome, we used a series of ANOVA and adonis (~PERMANOVA) models with Shannon diversity and community composition as the response variables, respectively. There was no statistically significant difference (KruskalWallies, p > 0.05) in bacterial diversity between the treated and untreated fruits. In this sense, the application of Clavispora lusitaneae 146 did not produce significant changes on bacterial communities of lemons during storage, including alpha diversity, community composition and structure. The bacterial community was dominated by roteobacteria,followed by Firmicutes and Actinobacteria. Specific bacterial taxa were only identified for untreated lemons: Methylobacteriaceae (Alphaproteobacteria) and unclassified bacteria, however in a low abundance. Here, we presented the first lemon microbiome and we showed that the microbial abundance, diversity, and community structures were not significantly different for both treatments, revealing that Clavispora lusitaniae 146 didn´t modify the native bacterial population of the fruit microbiome. The present study is part of larger project whose objectives are to define the complete lemon microbiome, assess the effects of the postharvest biocontrol agents on the composition of the lemon microbiome to develop a science-based strategy for manipulating this microbiome to prevent postharvest decay and physiological disorders.
Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
XVIII Congreso de la Sociedad Argentina de Microbiología General
Chapadmalal
Argentina
Sociedad Argentina de Microbiología General - Materia
-
MICROBIOME
LEMON
BIOCONTROL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/217235
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Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiomeRasuk, Maria CeciliaPisa, José HoracioChacón, Florencia IsabelPereyra, Martina MaríaDib, Julian RafaelMICROBIOMELEMONBIOCONTROLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The use of biocontrol agents has been proposed as an effective alternative to reduce citrus decays for promoting sustainable agriculture based on organic fruit production. Among the different microbial biocontrol agents, Clavispora lusitaniae 146 stands out as it is able of effectively controlling green mold in lemons. Although there is growing recognition of the role that the microbiome plays in the health and physiology of many plant species, to date, the composition of the lemon microbiome is unknown, nor is the effect of yeast 146 on it. Thus, the aim of this research was to study the impacts of biocontrol yeast Clavispora lusitaneae 146 on the composition of the lemon microbiome. Lemons were harvested, and then divided into two treatments: untreated and treated lemons with biocontrol yeast C. lusitaneae 146. Fruits were then stored at room temperature for 7 days. DNA was extracted from a pool of 3 pieces of peel per sample, and used for PCR that amplified the bacterial hypervariable V3-V4 region of the 16S rRNA gene. Paired-end sequencing of amplicons was done on an Illumina MiSeq sequencer. To assess the effects of postharvest treatment and storage on the diversity of the lemon microbiome, we used a series of ANOVA and adonis (~PERMANOVA) models with Shannon diversity and community composition as the response variables, respectively. There was no statistically significant difference (KruskalWallies, p > 0.05) in bacterial diversity between the treated and untreated fruits. In this sense, the application of Clavispora lusitaneae 146 did not produce significant changes on bacterial communities of lemons during storage, including alpha diversity, community composition and structure. The bacterial community was dominated by roteobacteria,followed by Firmicutes and Actinobacteria. Specific bacterial taxa were only identified for untreated lemons: Methylobacteriaceae (Alphaproteobacteria) and unclassified bacteria, however in a low abundance. Here, we presented the first lemon microbiome and we showed that the microbial abundance, diversity, and community structures were not significantly different for both treatments, revealing that Clavispora lusitaniae 146 didn´t modify the native bacterial population of the fruit microbiome. The present study is part of larger project whose objectives are to define the complete lemon microbiome, assess the effects of the postharvest biocontrol agents on the composition of the lemon microbiome to develop a science-based strategy for manipulating this microbiome to prevent postharvest decay and physiological disorders.Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaXVIII Congreso de la Sociedad Argentina de Microbiología GeneralChapadmalalArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217235Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome; XVIII Congreso de la Sociedad Argentina de Microbiología General; Chapadmalal; Argentina; 2023; 184-185CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2023/09/Libro-de-Resumenes-Final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:10Zoai:ri.conicet.gov.ar:11336/217235instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:11.076CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome |
title |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome |
spellingShingle |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome Rasuk, Maria Cecilia MICROBIOME LEMON BIOCONTROL |
title_short |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome |
title_full |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome |
title_fullStr |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome |
title_full_unstemmed |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome |
title_sort |
Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome |
dc.creator.none.fl_str_mv |
Rasuk, Maria Cecilia Pisa, José Horacio Chacón, Florencia Isabel Pereyra, Martina María Dib, Julian Rafael |
author |
Rasuk, Maria Cecilia |
author_facet |
Rasuk, Maria Cecilia Pisa, José Horacio Chacón, Florencia Isabel Pereyra, Martina María Dib, Julian Rafael |
author_role |
author |
author2 |
Pisa, José Horacio Chacón, Florencia Isabel Pereyra, Martina María Dib, Julian Rafael |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
MICROBIOME LEMON BIOCONTROL |
topic |
MICROBIOME LEMON BIOCONTROL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The use of biocontrol agents has been proposed as an effective alternative to reduce citrus decays for promoting sustainable agriculture based on organic fruit production. Among the different microbial biocontrol agents, Clavispora lusitaniae 146 stands out as it is able of effectively controlling green mold in lemons. Although there is growing recognition of the role that the microbiome plays in the health and physiology of many plant species, to date, the composition of the lemon microbiome is unknown, nor is the effect of yeast 146 on it. Thus, the aim of this research was to study the impacts of biocontrol yeast Clavispora lusitaneae 146 on the composition of the lemon microbiome. Lemons were harvested, and then divided into two treatments: untreated and treated lemons with biocontrol yeast C. lusitaneae 146. Fruits were then stored at room temperature for 7 days. DNA was extracted from a pool of 3 pieces of peel per sample, and used for PCR that amplified the bacterial hypervariable V3-V4 region of the 16S rRNA gene. Paired-end sequencing of amplicons was done on an Illumina MiSeq sequencer. To assess the effects of postharvest treatment and storage on the diversity of the lemon microbiome, we used a series of ANOVA and adonis (~PERMANOVA) models with Shannon diversity and community composition as the response variables, respectively. There was no statistically significant difference (KruskalWallies, p > 0.05) in bacterial diversity between the treated and untreated fruits. In this sense, the application of Clavispora lusitaneae 146 did not produce significant changes on bacterial communities of lemons during storage, including alpha diversity, community composition and structure. The bacterial community was dominated by roteobacteria,followed by Firmicutes and Actinobacteria. Specific bacterial taxa were only identified for untreated lemons: Methylobacteriaceae (Alphaproteobacteria) and unclassified bacteria, however in a low abundance. Here, we presented the first lemon microbiome and we showed that the microbial abundance, diversity, and community structures were not significantly different for both treatments, revealing that Clavispora lusitaniae 146 didn´t modify the native bacterial population of the fruit microbiome. The present study is part of larger project whose objectives are to define the complete lemon microbiome, assess the effects of the postharvest biocontrol agents on the composition of the lemon microbiome to develop a science-based strategy for manipulating this microbiome to prevent postharvest decay and physiological disorders. Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Chacón, Florencia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina XVIII Congreso de la Sociedad Argentina de Microbiología General Chapadmalal Argentina Sociedad Argentina de Microbiología General |
description |
The use of biocontrol agents has been proposed as an effective alternative to reduce citrus decays for promoting sustainable agriculture based on organic fruit production. Among the different microbial biocontrol agents, Clavispora lusitaniae 146 stands out as it is able of effectively controlling green mold in lemons. Although there is growing recognition of the role that the microbiome plays in the health and physiology of many plant species, to date, the composition of the lemon microbiome is unknown, nor is the effect of yeast 146 on it. Thus, the aim of this research was to study the impacts of biocontrol yeast Clavispora lusitaneae 146 on the composition of the lemon microbiome. Lemons were harvested, and then divided into two treatments: untreated and treated lemons with biocontrol yeast C. lusitaneae 146. Fruits were then stored at room temperature for 7 days. DNA was extracted from a pool of 3 pieces of peel per sample, and used for PCR that amplified the bacterial hypervariable V3-V4 region of the 16S rRNA gene. Paired-end sequencing of amplicons was done on an Illumina MiSeq sequencer. To assess the effects of postharvest treatment and storage on the diversity of the lemon microbiome, we used a series of ANOVA and adonis (~PERMANOVA) models with Shannon diversity and community composition as the response variables, respectively. There was no statistically significant difference (KruskalWallies, p > 0.05) in bacterial diversity between the treated and untreated fruits. In this sense, the application of Clavispora lusitaneae 146 did not produce significant changes on bacterial communities of lemons during storage, including alpha diversity, community composition and structure. The bacterial community was dominated by roteobacteria,followed by Firmicutes and Actinobacteria. Specific bacterial taxa were only identified for untreated lemons: Methylobacteriaceae (Alphaproteobacteria) and unclassified bacteria, however in a low abundance. Here, we presented the first lemon microbiome and we showed that the microbial abundance, diversity, and community structures were not significantly different for both treatments, revealing that Clavispora lusitaniae 146 didn´t modify the native bacterial population of the fruit microbiome. The present study is part of larger project whose objectives are to define the complete lemon microbiome, assess the effects of the postharvest biocontrol agents on the composition of the lemon microbiome to develop a science-based strategy for manipulating this microbiome to prevent postharvest decay and physiological disorders. |
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2023 |
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2023 |
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http://hdl.handle.net/11336/217235 Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome; XVIII Congreso de la Sociedad Argentina de Microbiología General; Chapadmalal; Argentina; 2023; 184-185 CONICET Digital CONICET |
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http://hdl.handle.net/11336/217235 |
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Impact of biocontrol yeast clavispora lusitaniae 146 on the lemon microbiome; XVIII Congreso de la Sociedad Argentina de Microbiología General; Chapadmalal; Argentina; 2023; 184-185 CONICET Digital CONICET |
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eng |
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eng |
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Internacional |
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Sociedad Argentina de Microbiología General |
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Sociedad Argentina de Microbiología General |
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