Local effects of low doses of pth 1-34 on experimental periodontitis

Autores
Zeni, Susana Noemi; Davison, Mario Ricardo; Bidevich, Nicolas L.; Preliasco, Mariana; Bonanno, Marina Soledad
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Periodontitis is a highly prevalent, chronic disease that induces a progressive bone resorption. Intermittent PTH administration has anabolic and anti-inflammatory effects, two properties necessary to achieve bone recovery. Periodontal disease can be experimentally induced in rats in few days, via cotton ligatures placement in the gingival sulcus around the molar teeth, that increases biofilm accumulation and disruption of the gingival epithelium, enhancing osteoclastogenesis and bone loss. We investigated whether intermittent administration of a low dose of PTH 1-34 in rats would block the alveolar bone loss observed when the ligature model of periodontitis was used. Periodontitis was induced in 16 female Wistar rats (221±15g) under light anesthesia. Ligature was replaced every 4 days. Rats were randomly divided in two groups and subcutaneously injected every 48 hs for 18 ds. with: G1 saline solution and G2 1.2ug PTH1-34. Eight rats were left unligated as healthy control. After killed hemimandibles were extracted and fixed in formalin buffer for histologic analysis of tibia subchondral bone volume (BV/TV%), alveolar bone BV/TV% and periodontal space height (PSH). Results: tibia BV/TV%: C 38.77±2.59; G1 38.29±3.9 and G2 37.75±1.45; alveolar bone BV/TV% C 50.3±3.6c; G1 35.6±4.3a and G2 42.0±1.4b; PSH (mm): C: 0.196±0.057a; G1 0.809±0.115b and G2: 0.706±0.065c. Different letters show a p<0.05. The results evidenced no systemic effect of PTH treatment on the tibia. Alveolar bone composed by trabecular bone showed a significant recovery. The PSH evidenced a little recovery but a greater percentage of osteoid tissue as compared to untreated rats. Conclusion: Intermittent low doses of PTH administration diminishes alveolar bone loss, but increases osteoid formation, suggesting that intermittent PTH administration attenuates periodontitis alveolar bone loss by the induction of tissue regeneration. Grant of Rio Negro National University. PI UNRN 40-A-467.
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Davison, Mario Ricardo. Universidad Nacional de Río Negro. Sede Alto Valle; Argentina
Fil: Bidevich, Nicolas L.. Universidad Nacional de Río Negro. Sede Alto Valle; Argentina
Fil: Preliasco, Mariana. Universidad Nacional de Río Negro. Sede Alto Valle; Argentina
Fil: Bonanno, Marina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología ; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Materia
PTH
Periodontitis
Osteología
HCNT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247666
Acceder
id CONICETDig_d316787caa05c5758bc4b88907d0bddb
oai_identifier_str oai:ri.conicet.gov.ar:11336/247666
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Local effects of low doses of pth 1-34 on experimental periodontitisZeni, Susana NoemiDavison, Mario RicardoBidevich, Nicolas L.Preliasco, MarianaBonanno, Marina SoledadPTHPeriodontitisOsteologíaHCNThttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Periodontitis is a highly prevalent, chronic disease that induces a progressive bone resorption. Intermittent PTH administration has anabolic and anti-inflammatory effects, two properties necessary to achieve bone recovery. Periodontal disease can be experimentally induced in rats in few days, via cotton ligatures placement in the gingival sulcus around the molar teeth, that increases biofilm accumulation and disruption of the gingival epithelium, enhancing osteoclastogenesis and bone loss. We investigated whether intermittent administration of a low dose of PTH 1-34 in rats would block the alveolar bone loss observed when the ligature model of periodontitis was used. Periodontitis was induced in 16 female Wistar rats (221±15g) under light anesthesia. Ligature was replaced every 4 days. Rats were randomly divided in two groups and subcutaneously injected every 48 hs for 18 ds. with: G1 saline solution and G2 1.2ug PTH1-34. Eight rats were left unligated as healthy control. After killed hemimandibles were extracted and fixed in formalin buffer for histologic analysis of tibia subchondral bone volume (BV/TV%), alveolar bone BV/TV% and periodontal space height (PSH). Results: tibia BV/TV%: C 38.77±2.59; G1 38.29±3.9 and G2 37.75±1.45; alveolar bone BV/TV% C 50.3±3.6c; G1 35.6±4.3a and G2 42.0±1.4b; PSH (mm): C: 0.196±0.057a; G1 0.809±0.115b and G2: 0.706±0.065c. Different letters show a p<0.05. The results evidenced no systemic effect of PTH treatment on the tibia. Alveolar bone composed by trabecular bone showed a significant recovery. The PSH evidenced a little recovery but a greater percentage of osteoid tissue as compared to untreated rats. Conclusion: Intermittent low doses of PTH administration diminishes alveolar bone loss, but increases osteoid formation, suggesting that intermittent PTH administration attenuates periodontitis alveolar bone loss by the induction of tissue regeneration. Grant of Rio Negro National University. PI UNRN 40-A-467.Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Davison, Mario Ricardo. Universidad Nacional de Río Negro. Sede Alto Valle; ArgentinaFil: Bidevich, Nicolas L.. Universidad Nacional de Río Negro. Sede Alto Valle; ArgentinaFil: Preliasco, Mariana. Universidad Nacional de Río Negro. Sede Alto Valle; ArgentinaFil: Bonanno, Marina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología ; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de NanomedicinasArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologiaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247666Local effects of low doses of pth 1-34 on experimental periodontitis; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología ; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Argentina; 2020; 1-50025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previasInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:23Zoai:ri.conicet.gov.ar:11336/247666instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:24.234CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Local effects of low doses of pth 1-34 on experimental periodontitis
title Local effects of low doses of pth 1-34 on experimental periodontitis
spellingShingle Local effects of low doses of pth 1-34 on experimental periodontitis
Zeni, Susana Noemi
PTH
Periodontitis
Osteología
HCNT
title_short Local effects of low doses of pth 1-34 on experimental periodontitis
title_full Local effects of low doses of pth 1-34 on experimental periodontitis
title_fullStr Local effects of low doses of pth 1-34 on experimental periodontitis
title_full_unstemmed Local effects of low doses of pth 1-34 on experimental periodontitis
title_sort Local effects of low doses of pth 1-34 on experimental periodontitis
dc.creator.none.fl_str_mv Zeni, Susana Noemi
Davison, Mario Ricardo
Bidevich, Nicolas L.
Preliasco, Mariana
Bonanno, Marina Soledad
author Zeni, Susana Noemi
author_facet Zeni, Susana Noemi
Davison, Mario Ricardo
Bidevich, Nicolas L.
Preliasco, Mariana
Bonanno, Marina Soledad
author_role author
author2 Davison, Mario Ricardo
Bidevich, Nicolas L.
Preliasco, Mariana
Bonanno, Marina Soledad
author2_role author
author
author
author
dc.subject.none.fl_str_mv PTH
Periodontitis
Osteología
HCNT
topic PTH
Periodontitis
Osteología
HCNT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Periodontitis is a highly prevalent, chronic disease that induces a progressive bone resorption. Intermittent PTH administration has anabolic and anti-inflammatory effects, two properties necessary to achieve bone recovery. Periodontal disease can be experimentally induced in rats in few days, via cotton ligatures placement in the gingival sulcus around the molar teeth, that increases biofilm accumulation and disruption of the gingival epithelium, enhancing osteoclastogenesis and bone loss. We investigated whether intermittent administration of a low dose of PTH 1-34 in rats would block the alveolar bone loss observed when the ligature model of periodontitis was used. Periodontitis was induced in 16 female Wistar rats (221±15g) under light anesthesia. Ligature was replaced every 4 days. Rats were randomly divided in two groups and subcutaneously injected every 48 hs for 18 ds. with: G1 saline solution and G2 1.2ug PTH1-34. Eight rats were left unligated as healthy control. After killed hemimandibles were extracted and fixed in formalin buffer for histologic analysis of tibia subchondral bone volume (BV/TV%), alveolar bone BV/TV% and periodontal space height (PSH). Results: tibia BV/TV%: C 38.77±2.59; G1 38.29±3.9 and G2 37.75±1.45; alveolar bone BV/TV% C 50.3±3.6c; G1 35.6±4.3a and G2 42.0±1.4b; PSH (mm): C: 0.196±0.057a; G1 0.809±0.115b and G2: 0.706±0.065c. Different letters show a p<0.05. The results evidenced no systemic effect of PTH treatment on the tibia. Alveolar bone composed by trabecular bone showed a significant recovery. The PSH evidenced a little recovery but a greater percentage of osteoid tissue as compared to untreated rats. Conclusion: Intermittent low doses of PTH administration diminishes alveolar bone loss, but increases osteoid formation, suggesting that intermittent PTH administration attenuates periodontitis alveolar bone loss by the induction of tissue regeneration. Grant of Rio Negro National University. PI UNRN 40-A-467.
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Davison, Mario Ricardo. Universidad Nacional de Río Negro. Sede Alto Valle; Argentina
Fil: Bidevich, Nicolas L.. Universidad Nacional de Río Negro. Sede Alto Valle; Argentina
Fil: Preliasco, Mariana. Universidad Nacional de Río Negro. Sede Alto Valle; Argentina
Fil: Bonanno, Marina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología ; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
description Periodontitis is a highly prevalent, chronic disease that induces a progressive bone resorption. Intermittent PTH administration has anabolic and anti-inflammatory effects, two properties necessary to achieve bone recovery. Periodontal disease can be experimentally induced in rats in few days, via cotton ligatures placement in the gingival sulcus around the molar teeth, that increases biofilm accumulation and disruption of the gingival epithelium, enhancing osteoclastogenesis and bone loss. We investigated whether intermittent administration of a low dose of PTH 1-34 in rats would block the alveolar bone loss observed when the ligature model of periodontitis was used. Periodontitis was induced in 16 female Wistar rats (221±15g) under light anesthesia. Ligature was replaced every 4 days. Rats were randomly divided in two groups and subcutaneously injected every 48 hs for 18 ds. with: G1 saline solution and G2 1.2ug PTH1-34. Eight rats were left unligated as healthy control. After killed hemimandibles were extracted and fixed in formalin buffer for histologic analysis of tibia subchondral bone volume (BV/TV%), alveolar bone BV/TV% and periodontal space height (PSH). Results: tibia BV/TV%: C 38.77±2.59; G1 38.29±3.9 and G2 37.75±1.45; alveolar bone BV/TV% C 50.3±3.6c; G1 35.6±4.3a and G2 42.0±1.4b; PSH (mm): C: 0.196±0.057a; G1 0.809±0.115b and G2: 0.706±0.065c. Different letters show a p<0.05. The results evidenced no systemic effect of PTH treatment on the tibia. Alveolar bone composed by trabecular bone showed a significant recovery. The PSH evidenced a little recovery but a greater percentage of osteoid tissue as compared to untreated rats. Conclusion: Intermittent low doses of PTH administration diminishes alveolar bone loss, but increases osteoid formation, suggesting that intermittent PTH administration attenuates periodontitis alveolar bone loss by the induction of tissue regeneration. Grant of Rio Negro National University. PI UNRN 40-A-467.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247666
Local effects of low doses of pth 1-34 on experimental periodontitis; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología ; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Argentina; 2020; 1-5
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247666
identifier_str_mv Local effects of low doses of pth 1-34 on experimental periodontitis; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología ; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Argentina; 2020; 1-5
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previas
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781646380990464
score 12.982451

Publicaciones similares