Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo

Autores
Escobar, Franco Matias; Cariddi, Laura Noelia; Sabini, Maria Carola; Reinoso, Elina Beatríz; Sutil, Sonia Beatriz; Torres, Cristina Vanesa; Zanon, Silvia Matilde; Sabini, Liliana Inés
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Minthostachys verticillata (peperina) is an aromatic and medicinal plant with several uses and ethnobotanical properties. Numerous studies have demonstrated that its essential oil (Mv-EO) presents antimicrobial capacity and shows immunomodulating and anti-allergic properties in human cell lines. Thus, the goal of this study was to investigate the main chemical composition, analyzed by GC?FID, and the cyto-genotoxic effects of Mv-EO, using Vero cells, human PBMCs and mice bone marrow cells. The Mv-EO was rich in pulegone 60.5% and menthone 18.2%. Our results clearly show that Mv-EO is not cyto-genotoxic in vitro nor in vivo. It not induced cytotoxic effects, as indicated by trypan blue dye exclusion and NRU assays both in Vero cells and human PBMCs. In addition, Mv-EO (100?1000 μg/mL) not induced apoptotic effects on human PBMCs, as indicated by Hoechst staining and DNA fragmentation analysis by agarose gel electrophoresis. The in vivo assay showed that Mv-EO (25?500 mg/kg) not increased the frequency of micronucleus in bone marrow cells of mice. Further, the ratio of polychromatic/normochromatic erythrocytes was not modified. These findings suggest that Mv-EO appears to be safe as a therapeutic agent.
Fil: Escobar, Franco Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sutil, Sonia Beatriz. Universidad Nacional de Río Cuarto; Argentina
Fil: Torres, Cristina Vanesa. Universidad Nacional de Río Cuarto; Argentina
Fil: Zanon, Silvia Matilde. Universidad Nacional de Río Cuarto; Argentina
Fil: Sabini, Liliana Inés. Universidad Nacional de Río Cuarto; Argentina
Materia
APOPTOSIS
CYTOTOXICITY
ESSENTIAL OIL
GAS CHROMATOGRAPHY
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199133
Acceder
id CONICETDig_d29962ef9eb1784894d73780fc347f80
oai_identifier_str oai:ri.conicet.gov.ar:11336/199133
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivoEscobar, Franco MatiasCariddi, Laura NoeliaSabini, Maria CarolaReinoso, Elina BeatrízSutil, Sonia BeatrizTorres, Cristina VanesaZanon, Silvia MatildeSabini, Liliana InésAPOPTOSISCYTOTOXICITYESSENTIAL OILGAS CHROMATOGRAPHYGENOTOXICITYMINTHOSTACHYS VERTICILLATAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Minthostachys verticillata (peperina) is an aromatic and medicinal plant with several uses and ethnobotanical properties. Numerous studies have demonstrated that its essential oil (Mv-EO) presents antimicrobial capacity and shows immunomodulating and anti-allergic properties in human cell lines. Thus, the goal of this study was to investigate the main chemical composition, analyzed by GC?FID, and the cyto-genotoxic effects of Mv-EO, using Vero cells, human PBMCs and mice bone marrow cells. The Mv-EO was rich in pulegone 60.5% and menthone 18.2%. Our results clearly show that Mv-EO is not cyto-genotoxic in vitro nor in vivo. It not induced cytotoxic effects, as indicated by trypan blue dye exclusion and NRU assays both in Vero cells and human PBMCs. In addition, Mv-EO (100?1000 μg/mL) not induced apoptotic effects on human PBMCs, as indicated by Hoechst staining and DNA fragmentation analysis by agarose gel electrophoresis. The in vivo assay showed that Mv-EO (25?500 mg/kg) not increased the frequency of micronucleus in bone marrow cells of mice. Further, the ratio of polychromatic/normochromatic erythrocytes was not modified. These findings suggest that Mv-EO appears to be safe as a therapeutic agent.Fil: Escobar, Franco Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Sutil, Sonia Beatriz. Universidad Nacional de Río Cuarto; ArgentinaFil: Torres, Cristina Vanesa. Universidad Nacional de Río Cuarto; ArgentinaFil: Zanon, Silvia Matilde. Universidad Nacional de Río Cuarto; ArgentinaFil: Sabini, Liliana Inés. Universidad Nacional de Río Cuarto; ArgentinaPergamon-Elsevier Science Ltd2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199133Escobar, Franco Matias; Cariddi, Laura Noelia; Sabini, Maria Carola; Reinoso, Elina Beatríz; Sutil, Sonia Beatriz; et al.; Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo; Pergamon-Elsevier Science Ltd; Food and Chemical Toxicology; 50; 9; 9-2012; 3062-30670278-6915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0278691512004322info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fct.2012.06.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:27Zoai:ri.conicet.gov.ar:11336/199133instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:27.389CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
title Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
spellingShingle Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
Escobar, Franco Matias
APOPTOSIS
CYTOTOXICITY
ESSENTIAL OIL
GAS CHROMATOGRAPHY
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
title_short Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
title_full Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
title_fullStr Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
title_full_unstemmed Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
title_sort Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo
dc.creator.none.fl_str_mv Escobar, Franco Matias
Cariddi, Laura Noelia
Sabini, Maria Carola
Reinoso, Elina Beatríz
Sutil, Sonia Beatriz
Torres, Cristina Vanesa
Zanon, Silvia Matilde
Sabini, Liliana Inés
author Escobar, Franco Matias
author_facet Escobar, Franco Matias
Cariddi, Laura Noelia
Sabini, Maria Carola
Reinoso, Elina Beatríz
Sutil, Sonia Beatriz
Torres, Cristina Vanesa
Zanon, Silvia Matilde
Sabini, Liliana Inés
author_role author
author2 Cariddi, Laura Noelia
Sabini, Maria Carola
Reinoso, Elina Beatríz
Sutil, Sonia Beatriz
Torres, Cristina Vanesa
Zanon, Silvia Matilde
Sabini, Liliana Inés
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
CYTOTOXICITY
ESSENTIAL OIL
GAS CHROMATOGRAPHY
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
topic APOPTOSIS
CYTOTOXICITY
ESSENTIAL OIL
GAS CHROMATOGRAPHY
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Minthostachys verticillata (peperina) is an aromatic and medicinal plant with several uses and ethnobotanical properties. Numerous studies have demonstrated that its essential oil (Mv-EO) presents antimicrobial capacity and shows immunomodulating and anti-allergic properties in human cell lines. Thus, the goal of this study was to investigate the main chemical composition, analyzed by GC?FID, and the cyto-genotoxic effects of Mv-EO, using Vero cells, human PBMCs and mice bone marrow cells. The Mv-EO was rich in pulegone 60.5% and menthone 18.2%. Our results clearly show that Mv-EO is not cyto-genotoxic in vitro nor in vivo. It not induced cytotoxic effects, as indicated by trypan blue dye exclusion and NRU assays both in Vero cells and human PBMCs. In addition, Mv-EO (100?1000 μg/mL) not induced apoptotic effects on human PBMCs, as indicated by Hoechst staining and DNA fragmentation analysis by agarose gel electrophoresis. The in vivo assay showed that Mv-EO (25?500 mg/kg) not increased the frequency of micronucleus in bone marrow cells of mice. Further, the ratio of polychromatic/normochromatic erythrocytes was not modified. These findings suggest that Mv-EO appears to be safe as a therapeutic agent.
Fil: Escobar, Franco Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sutil, Sonia Beatriz. Universidad Nacional de Río Cuarto; Argentina
Fil: Torres, Cristina Vanesa. Universidad Nacional de Río Cuarto; Argentina
Fil: Zanon, Silvia Matilde. Universidad Nacional de Río Cuarto; Argentina
Fil: Sabini, Liliana Inés. Universidad Nacional de Río Cuarto; Argentina
description Minthostachys verticillata (peperina) is an aromatic and medicinal plant with several uses and ethnobotanical properties. Numerous studies have demonstrated that its essential oil (Mv-EO) presents antimicrobial capacity and shows immunomodulating and anti-allergic properties in human cell lines. Thus, the goal of this study was to investigate the main chemical composition, analyzed by GC?FID, and the cyto-genotoxic effects of Mv-EO, using Vero cells, human PBMCs and mice bone marrow cells. The Mv-EO was rich in pulegone 60.5% and menthone 18.2%. Our results clearly show that Mv-EO is not cyto-genotoxic in vitro nor in vivo. It not induced cytotoxic effects, as indicated by trypan blue dye exclusion and NRU assays both in Vero cells and human PBMCs. In addition, Mv-EO (100?1000 μg/mL) not induced apoptotic effects on human PBMCs, as indicated by Hoechst staining and DNA fragmentation analysis by agarose gel electrophoresis. The in vivo assay showed that Mv-EO (25?500 mg/kg) not increased the frequency of micronucleus in bone marrow cells of mice. Further, the ratio of polychromatic/normochromatic erythrocytes was not modified. These findings suggest that Mv-EO appears to be safe as a therapeutic agent.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199133
Escobar, Franco Matias; Cariddi, Laura Noelia; Sabini, Maria Carola; Reinoso, Elina Beatríz; Sutil, Sonia Beatriz; et al.; Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo; Pergamon-Elsevier Science Ltd; Food and Chemical Toxicology; 50; 9; 9-2012; 3062-3067
0278-6915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199133
identifier_str_mv Escobar, Franco Matias; Cariddi, Laura Noelia; Sabini, Maria Carola; Reinoso, Elina Beatríz; Sutil, Sonia Beatriz; et al.; Lack of cytotoxic and genotoxic effects of Minthostachys verticillata essential oil: Studies in vitro and in vivo; Pergamon-Elsevier Science Ltd; Food and Chemical Toxicology; 50; 9; 9-2012; 3062-3067
0278-6915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0278691512004322
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fct.2012.06.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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