Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity

Autores
Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; Soltermann, Arnaldo Teseo; Cavaglieri, Lilia Reneé
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive.
Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bagnis, Guillermo. Universidad Nacional de Río Cuarto; Argentina
Fil: Soltermann, Arnaldo Teseo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
AFLATOXINS
CYTOTOXICITY
ESSENTIAL OIL
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
PHYTOGENIC FEED ADDITIVE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/124247
Acceder
id CONICETDig_8021bec4174abfc589952690c2c60c72
oai_identifier_str oai:ri.conicet.gov.ar:11336/124247
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicityEscobar, Franco MatiasMagnoli, Alejandra PaolaSabini, Maria CarolaCariddi, Laura NoeliaBagnis, GuillermoSoltermann, Arnaldo TeseoCavaglieri, Lilia ReneéAFLATOXINSCYTOTOXICITYESSENTIAL OILGENOTOXICITYMINTHOSTACHYS VERTICILLATAPHYTOGENIC FEED ADDITIVEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive.Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Bagnis, Guillermo. Universidad Nacional de Río Cuarto; ArgentinaFil: Soltermann, Arnaldo Teseo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaTaylor & Francis2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124247Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; et al.; Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity; Taylor & Francis; Journal Of Applied Animal Research; 47; 1; 1-2019; 217-2220971-2119CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09712119.2019.1614929info:eu-repo/semantics/altIdentifier/doi/10.1080/09712119.2019.1614929info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:26Zoai:ri.conicet.gov.ar:11336/124247instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:26.779CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
title Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
spellingShingle Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
Escobar, Franco Matias
AFLATOXINS
CYTOTOXICITY
ESSENTIAL OIL
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
PHYTOGENIC FEED ADDITIVE
title_short Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
title_full Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
title_fullStr Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
title_full_unstemmed Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
title_sort Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
dc.creator.none.fl_str_mv Escobar, Franco Matias
Magnoli, Alejandra Paola
Sabini, Maria Carola
Cariddi, Laura Noelia
Bagnis, Guillermo
Soltermann, Arnaldo Teseo
Cavaglieri, Lilia Reneé
author Escobar, Franco Matias
author_facet Escobar, Franco Matias
Magnoli, Alejandra Paola
Sabini, Maria Carola
Cariddi, Laura Noelia
Bagnis, Guillermo
Soltermann, Arnaldo Teseo
Cavaglieri, Lilia Reneé
author_role author
author2 Magnoli, Alejandra Paola
Sabini, Maria Carola
Cariddi, Laura Noelia
Bagnis, Guillermo
Soltermann, Arnaldo Teseo
Cavaglieri, Lilia Reneé
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AFLATOXINS
CYTOTOXICITY
ESSENTIAL OIL
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
PHYTOGENIC FEED ADDITIVE
topic AFLATOXINS
CYTOTOXICITY
ESSENTIAL OIL
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
PHYTOGENIC FEED ADDITIVE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive.
Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bagnis, Guillermo. Universidad Nacional de Río Cuarto; Argentina
Fil: Soltermann, Arnaldo Teseo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/124247
Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; et al.; Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity; Taylor & Francis; Journal Of Applied Animal Research; 47; 1; 1-2019; 217-222
0971-2119
CONICET Digital
CONICET
url http://hdl.handle.net/11336/124247
identifier_str_mv Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; et al.; Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity; Taylor & Francis; Journal Of Applied Animal Research; 47; 1; 1-2019; 217-222
0971-2119
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09712119.2019.1614929
info:eu-repo/semantics/altIdentifier/doi/10.1080/09712119.2019.1614929
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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