Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity
- Autores
- Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; Soltermann, Arnaldo Teseo; Cavaglieri, Lilia Reneé
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive.
Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bagnis, Guillermo. Universidad Nacional de Río Cuarto; Argentina
Fil: Soltermann, Arnaldo Teseo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina - Materia
-
AFLATOXINS
CYTOTOXICITY
ESSENTIAL OIL
GENOTOXICITY
MINTHOSTACHYS VERTICILLATA
PHYTOGENIC FEED ADDITIVE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/124247
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spelling |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicityEscobar, Franco MatiasMagnoli, Alejandra PaolaSabini, Maria CarolaCariddi, Laura NoeliaBagnis, GuillermoSoltermann, Arnaldo TeseoCavaglieri, Lilia ReneéAFLATOXINSCYTOTOXICITYESSENTIAL OILGENOTOXICITYMINTHOSTACHYS VERTICILLATAPHYTOGENIC FEED ADDITIVEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive.Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Bagnis, Guillermo. Universidad Nacional de Río Cuarto; ArgentinaFil: Soltermann, Arnaldo Teseo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaTaylor & Francis2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124247Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; et al.; Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity; Taylor & Francis; Journal Of Applied Animal Research; 47; 1; 1-2019; 217-2220971-2119CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09712119.2019.1614929info:eu-repo/semantics/altIdentifier/doi/10.1080/09712119.2019.1614929info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:40Zoai:ri.conicet.gov.ar:11336/124247instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:40.575CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity |
title |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity |
spellingShingle |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity Escobar, Franco Matias AFLATOXINS CYTOTOXICITY ESSENTIAL OIL GENOTOXICITY MINTHOSTACHYS VERTICILLATA PHYTOGENIC FEED ADDITIVE |
title_short |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity |
title_full |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity |
title_fullStr |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity |
title_full_unstemmed |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity |
title_sort |
Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity |
dc.creator.none.fl_str_mv |
Escobar, Franco Matias Magnoli, Alejandra Paola Sabini, Maria Carola Cariddi, Laura Noelia Bagnis, Guillermo Soltermann, Arnaldo Teseo Cavaglieri, Lilia Reneé |
author |
Escobar, Franco Matias |
author_facet |
Escobar, Franco Matias Magnoli, Alejandra Paola Sabini, Maria Carola Cariddi, Laura Noelia Bagnis, Guillermo Soltermann, Arnaldo Teseo Cavaglieri, Lilia Reneé |
author_role |
author |
author2 |
Magnoli, Alejandra Paola Sabini, Maria Carola Cariddi, Laura Noelia Bagnis, Guillermo Soltermann, Arnaldo Teseo Cavaglieri, Lilia Reneé |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
AFLATOXINS CYTOTOXICITY ESSENTIAL OIL GENOTOXICITY MINTHOSTACHYS VERTICILLATA PHYTOGENIC FEED ADDITIVE |
topic |
AFLATOXINS CYTOTOXICITY ESSENTIAL OIL GENOTOXICITY MINTHOSTACHYS VERTICILLATA PHYTOGENIC FEED ADDITIVE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive. Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Bagnis, Guillermo. Universidad Nacional de Río Cuarto; Argentina Fil: Soltermann, Arnaldo Teseo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina |
description |
The aim of the current study was to evaluate the protective effects of Minthostachys verticillata essential oil (Mv-EO) against aflatoxin B1-induced toxicity in Vero cells in vitro and in male rats. The cytotoxicity of AFB1 (0-30 µg/mL) and Mv-EO (0-500 µg/mL) was determined on Vero cells using the Neutral Red assays. The tested Mv-EO did not cause genotoxicity or cytotoxicity in vivo and was able to attenuate AFB1-caused cytotoxicity and genotoxicity. Animals were divided into four groups consisting of 5 (five) rats in each group: T1 basal feed (BF – negative control), T2 BF + Mv-EO [0.04%], T3 BF + AFB1 [100 µg/kg], T4 BF + food with AFB1 [100 µg/kg] + Mv-EO [0.04%]. Tissue samples were collected at the end of treatment period for genotoxic study and histological examination. Treatment with Mv-EO alone and even combined with AFB1 showed a significant improvement in the histomorphometry of intestinal villi, without alteration of productive parameters. Also, the micronucleus test demonstrated that Mv-EO reduced AFB1-induced DNA damage on bone marrow cells of male Wistar rats. This study demonstrated that Mv-EO could be used as protective against AFB1-induced cytotoxicity and genotoxicity, and as phytogenic feed additive. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/124247 Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; et al.; Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity; Taylor & Francis; Journal Of Applied Animal Research; 47; 1; 1-2019; 217-222 0971-2119 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/124247 |
identifier_str_mv |
Escobar, Franco Matias; Magnoli, Alejandra Paola; Sabini, Maria Carola; Cariddi, Laura Noelia; Bagnis, Guillermo; et al.; Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity; Taylor & Francis; Journal Of Applied Animal Research; 47; 1; 1-2019; 217-222 0971-2119 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09712119.2019.1614929 info:eu-repo/semantics/altIdentifier/doi/10.1080/09712119.2019.1614929 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |