Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans

Autores
Kronberg, Maria Florencia; Clavijo Lara, Araceli Maria; Moya, Aldana; Rossen, Ariana Altair; Calvo, Daniel; Pagano, Eduardo Antonio; Munarriz, Eliana Rosa
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glyphosate-based formulation is used as non-selective and post-emergent herbicides in urban and rural activities. In view of its recurring applications in agricultural producing countries, the increase of glyphosate concentration in the environment stresses the need to test the adverse effects on non-target organisms and assess the risk of its use. This paper analyzes the toxicological and oxidative stress and modulatory effects of a glyphosate commercial formulation (glyphosate F) on the nematode Caenorhabditis elegans. We detected ROS production and enhancement of oxidative stress response in glyphosate F-treated nematodes. Particularly, we found an increased ctl-1 catalase gene expression of a catalase specific activity. In addition, we showed that glyphosate F treatment activated the FOXO transcription factor DAF-16, a critical target of the insulin/IGF-1 signaling pathway, which modulates the transcription of a broad range of genes involved in stress resistance, reproductive development, dauer formation, and longevity. In summary, the exposure of glyphosate F induces an oxidative imbalance in C. elegans that leads to the DAF-16 activation and consequently to the expression of genes that boost the antioxidant defense system. In this regard, clt-1 gene and catalase activity proved to be excellent biomarkers to develop more sensitive protocols to assess the environmental risk of glyphosate use.
Fil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Clavijo Lara, Araceli Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Moya, Aldana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Rossen, Ariana Altair. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional del Agua; Argentina
Fil: Calvo, Daniel. Instituto Nacional del Agua; Argentina
Fil: Pagano, Eduardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Munarriz, Eliana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Materia
AGROCHEMICAL
CATALASE
DAF-16
HORMESIS
PESTICIDE
ROS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93269
Acceder
id CONICETDig_d2674b3625f2e27e41357ebc9fb82580
oai_identifier_str oai:ri.conicet.gov.ar:11336/93269
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegansKronberg, Maria FlorenciaClavijo Lara, Araceli MariaMoya, AldanaRossen, Ariana AltairCalvo, DanielPagano, Eduardo AntonioMunarriz, Eliana RosaAGROCHEMICALCATALASEDAF-16HORMESISPESTICIDEROShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Glyphosate-based formulation is used as non-selective and post-emergent herbicides in urban and rural activities. In view of its recurring applications in agricultural producing countries, the increase of glyphosate concentration in the environment stresses the need to test the adverse effects on non-target organisms and assess the risk of its use. This paper analyzes the toxicological and oxidative stress and modulatory effects of a glyphosate commercial formulation (glyphosate F) on the nematode Caenorhabditis elegans. We detected ROS production and enhancement of oxidative stress response in glyphosate F-treated nematodes. Particularly, we found an increased ctl-1 catalase gene expression of a catalase specific activity. In addition, we showed that glyphosate F treatment activated the FOXO transcription factor DAF-16, a critical target of the insulin/IGF-1 signaling pathway, which modulates the transcription of a broad range of genes involved in stress resistance, reproductive development, dauer formation, and longevity. In summary, the exposure of glyphosate F induces an oxidative imbalance in C. elegans that leads to the DAF-16 activation and consequently to the expression of genes that boost the antioxidant defense system. In this regard, clt-1 gene and catalase activity proved to be excellent biomarkers to develop more sensitive protocols to assess the environmental risk of glyphosate use.Fil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Clavijo Lara, Araceli Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Moya, Aldana. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Rossen, Ariana Altair. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional del Agua; ArgentinaFil: Calvo, Daniel. Instituto Nacional del Agua; ArgentinaFil: Pagano, Eduardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Munarriz, Eliana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaElsevier Science Inc2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93269Kronberg, Maria Florencia; Clavijo Lara, Araceli Maria; Moya, Aldana; Rossen, Ariana Altair; Calvo, Daniel; et al.; Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 214; 12-2018; 1-81532-0456CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2018.08.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045618301145info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:52Zoai:ri.conicet.gov.ar:11336/93269instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:52.536CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
title Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
spellingShingle Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
Kronberg, Maria Florencia
AGROCHEMICAL
CATALASE
DAF-16
HORMESIS
PESTICIDE
ROS
title_short Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
title_full Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
title_fullStr Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
title_full_unstemmed Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
title_sort Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans
dc.creator.none.fl_str_mv Kronberg, Maria Florencia
Clavijo Lara, Araceli Maria
Moya, Aldana
Rossen, Ariana Altair
Calvo, Daniel
Pagano, Eduardo Antonio
Munarriz, Eliana Rosa
author Kronberg, Maria Florencia
author_facet Kronberg, Maria Florencia
Clavijo Lara, Araceli Maria
Moya, Aldana
Rossen, Ariana Altair
Calvo, Daniel
Pagano, Eduardo Antonio
Munarriz, Eliana Rosa
author_role author
author2 Clavijo Lara, Araceli Maria
Moya, Aldana
Rossen, Ariana Altair
Calvo, Daniel
Pagano, Eduardo Antonio
Munarriz, Eliana Rosa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AGROCHEMICAL
CATALASE
DAF-16
HORMESIS
PESTICIDE
ROS
topic AGROCHEMICAL
CATALASE
DAF-16
HORMESIS
PESTICIDE
ROS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Glyphosate-based formulation is used as non-selective and post-emergent herbicides in urban and rural activities. In view of its recurring applications in agricultural producing countries, the increase of glyphosate concentration in the environment stresses the need to test the adverse effects on non-target organisms and assess the risk of its use. This paper analyzes the toxicological and oxidative stress and modulatory effects of a glyphosate commercial formulation (glyphosate F) on the nematode Caenorhabditis elegans. We detected ROS production and enhancement of oxidative stress response in glyphosate F-treated nematodes. Particularly, we found an increased ctl-1 catalase gene expression of a catalase specific activity. In addition, we showed that glyphosate F treatment activated the FOXO transcription factor DAF-16, a critical target of the insulin/IGF-1 signaling pathway, which modulates the transcription of a broad range of genes involved in stress resistance, reproductive development, dauer formation, and longevity. In summary, the exposure of glyphosate F induces an oxidative imbalance in C. elegans that leads to the DAF-16 activation and consequently to the expression of genes that boost the antioxidant defense system. In this regard, clt-1 gene and catalase activity proved to be excellent biomarkers to develop more sensitive protocols to assess the environmental risk of glyphosate use.
Fil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Clavijo Lara, Araceli Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Moya, Aldana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Rossen, Ariana Altair. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional del Agua; Argentina
Fil: Calvo, Daniel. Instituto Nacional del Agua; Argentina
Fil: Pagano, Eduardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Munarriz, Eliana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
description Glyphosate-based formulation is used as non-selective and post-emergent herbicides in urban and rural activities. In view of its recurring applications in agricultural producing countries, the increase of glyphosate concentration in the environment stresses the need to test the adverse effects on non-target organisms and assess the risk of its use. This paper analyzes the toxicological and oxidative stress and modulatory effects of a glyphosate commercial formulation (glyphosate F) on the nematode Caenorhabditis elegans. We detected ROS production and enhancement of oxidative stress response in glyphosate F-treated nematodes. Particularly, we found an increased ctl-1 catalase gene expression of a catalase specific activity. In addition, we showed that glyphosate F treatment activated the FOXO transcription factor DAF-16, a critical target of the insulin/IGF-1 signaling pathway, which modulates the transcription of a broad range of genes involved in stress resistance, reproductive development, dauer formation, and longevity. In summary, the exposure of glyphosate F induces an oxidative imbalance in C. elegans that leads to the DAF-16 activation and consequently to the expression of genes that boost the antioxidant defense system. In this regard, clt-1 gene and catalase activity proved to be excellent biomarkers to develop more sensitive protocols to assess the environmental risk of glyphosate use.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93269
Kronberg, Maria Florencia; Clavijo Lara, Araceli Maria; Moya, Aldana; Rossen, Ariana Altair; Calvo, Daniel; et al.; Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 214; 12-2018; 1-8
1532-0456
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93269
identifier_str_mv Kronberg, Maria Florencia; Clavijo Lara, Araceli Maria; Moya, Aldana; Rossen, Ariana Altair; Calvo, Daniel; et al.; Glyphosate-based herbicides modulate oxidative stress response in the nematode Caenorhabditis elegans; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 214; 12-2018; 1-8
1532-0456
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2018.08.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045618301145
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268629446426624
score 13.13397

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