Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses

Autores
Cuesta, Santiago; Burdisso, Paula; Segev, Amir; Kourrich, Saïd; Sperandio, Vanessa
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gut-microbiota membership is associated with diverse neuropsychological outcomes, including substance use disorders (SUDs). Here, we use mice colonized with Citrobacter rodentium or the human γ-Proteobacteria commensal Escherichia coli HS as a model to examine the mechanistic interactions between gut microbes and host responses to cocaine. We find that cocaine exposure increases intestinal norepinephrine levels that are sensed through the bacterial adrenergic receptor QseC to promote intestinal colonization of γ-Proteobacteria. Colonized mice show enhanced host cocaine-induced behaviors. The neuroactive metabolite glycine, a bacterial nitrogen source, is depleted in the gut and cerebrospinal fluid of colonized mice. Systemic glycine repletion reversed, and γ-Proteobacteria mutated for glycine uptake did not alter the host response to cocaine. γ-Proteobacteria modulated glycine levels are linked to cocaine-induced transcriptional plasticity in the nucleus accumbens through glutamatergic transmission. The mechanism outline here could potentially be exploited to modulate reward-related brain circuits that contribute to SUDs.
Fil: Cuesta, Santiago. University of Texas. Southwestern Medical Center; Estados Unidos
Fil: Burdisso, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Segev, Amir. University Of Texas Southwestern Medical School,; Estados Unidos. University of Texas; Estados Unidos
Fil: Kourrich, Saïd. Université du Québec a Montreal; Canadá
Fil: Sperandio, Vanessa. University of Texas. Southwestern Medical Center; Estados Unidos
Materia
CITROBACTER RODENTIUM
COCAINE
GLYCINE
GUT-BRAIN AXIS
HOST-MICROBE INTERACTIONS
MICROBIOTA
NOREPINEPHRINE
PROTEOBACTERIA
QSEC
SUBSTANCE ABUSE DISORDERS (SUDS)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213381
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/213381
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responsesCuesta, SantiagoBurdisso, PaulaSegev, AmirKourrich, SaïdSperandio, VanessaCITROBACTER RODENTIUMCOCAINEGLYCINEGUT-BRAIN AXISHOST-MICROBE INTERACTIONSMICROBIOTANOREPINEPHRINEPROTEOBACTERIAQSECSUBSTANCE ABUSE DISORDERS (SUDS)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gut-microbiota membership is associated with diverse neuropsychological outcomes, including substance use disorders (SUDs). Here, we use mice colonized with Citrobacter rodentium or the human γ-Proteobacteria commensal Escherichia coli HS as a model to examine the mechanistic interactions between gut microbes and host responses to cocaine. We find that cocaine exposure increases intestinal norepinephrine levels that are sensed through the bacterial adrenergic receptor QseC to promote intestinal colonization of γ-Proteobacteria. Colonized mice show enhanced host cocaine-induced behaviors. The neuroactive metabolite glycine, a bacterial nitrogen source, is depleted in the gut and cerebrospinal fluid of colonized mice. Systemic glycine repletion reversed, and γ-Proteobacteria mutated for glycine uptake did not alter the host response to cocaine. γ-Proteobacteria modulated glycine levels are linked to cocaine-induced transcriptional plasticity in the nucleus accumbens through glutamatergic transmission. The mechanism outline here could potentially be exploited to modulate reward-related brain circuits that contribute to SUDs.Fil: Cuesta, Santiago. University of Texas. Southwestern Medical Center; Estados UnidosFil: Burdisso, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Segev, Amir. University Of Texas Southwestern Medical School,; Estados Unidos. University of Texas; Estados UnidosFil: Kourrich, Saïd. Université du Québec a Montreal; CanadáFil: Sperandio, Vanessa. University of Texas. Southwestern Medical Center; Estados UnidosCell Press2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213381Cuesta, Santiago; Burdisso, Paula; Segev, Amir; Kourrich, Saïd; Sperandio, Vanessa; Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses; Cell Press; Cell Host & Microbe; 30; 11; 11-2022; 1615-1629.e51931-3128CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S193131282200470Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chom.2022.09.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:22Zoai:ri.conicet.gov.ar:11336/213381instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:23.019CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
title Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
spellingShingle Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
Cuesta, Santiago
CITROBACTER RODENTIUM
COCAINE
GLYCINE
GUT-BRAIN AXIS
HOST-MICROBE INTERACTIONS
MICROBIOTA
NOREPINEPHRINE
PROTEOBACTERIA
QSEC
SUBSTANCE ABUSE DISORDERS (SUDS)
title_short Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
title_full Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
title_fullStr Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
title_full_unstemmed Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
title_sort Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses
dc.creator.none.fl_str_mv Cuesta, Santiago
Burdisso, Paula
Segev, Amir
Kourrich, Saïd
Sperandio, Vanessa
author Cuesta, Santiago
author_facet Cuesta, Santiago
Burdisso, Paula
Segev, Amir
Kourrich, Saïd
Sperandio, Vanessa
author_role author
author2 Burdisso, Paula
Segev, Amir
Kourrich, Saïd
Sperandio, Vanessa
author2_role author
author
author
author
dc.subject.none.fl_str_mv CITROBACTER RODENTIUM
COCAINE
GLYCINE
GUT-BRAIN AXIS
HOST-MICROBE INTERACTIONS
MICROBIOTA
NOREPINEPHRINE
PROTEOBACTERIA
QSEC
SUBSTANCE ABUSE DISORDERS (SUDS)
topic CITROBACTER RODENTIUM
COCAINE
GLYCINE
GUT-BRAIN AXIS
HOST-MICROBE INTERACTIONS
MICROBIOTA
NOREPINEPHRINE
PROTEOBACTERIA
QSEC
SUBSTANCE ABUSE DISORDERS (SUDS)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gut-microbiota membership is associated with diverse neuropsychological outcomes, including substance use disorders (SUDs). Here, we use mice colonized with Citrobacter rodentium or the human γ-Proteobacteria commensal Escherichia coli HS as a model to examine the mechanistic interactions between gut microbes and host responses to cocaine. We find that cocaine exposure increases intestinal norepinephrine levels that are sensed through the bacterial adrenergic receptor QseC to promote intestinal colonization of γ-Proteobacteria. Colonized mice show enhanced host cocaine-induced behaviors. The neuroactive metabolite glycine, a bacterial nitrogen source, is depleted in the gut and cerebrospinal fluid of colonized mice. Systemic glycine repletion reversed, and γ-Proteobacteria mutated for glycine uptake did not alter the host response to cocaine. γ-Proteobacteria modulated glycine levels are linked to cocaine-induced transcriptional plasticity in the nucleus accumbens through glutamatergic transmission. The mechanism outline here could potentially be exploited to modulate reward-related brain circuits that contribute to SUDs.
Fil: Cuesta, Santiago. University of Texas. Southwestern Medical Center; Estados Unidos
Fil: Burdisso, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Segev, Amir. University Of Texas Southwestern Medical School,; Estados Unidos. University of Texas; Estados Unidos
Fil: Kourrich, Saïd. Université du Québec a Montreal; Canadá
Fil: Sperandio, Vanessa. University of Texas. Southwestern Medical Center; Estados Unidos
description Gut-microbiota membership is associated with diverse neuropsychological outcomes, including substance use disorders (SUDs). Here, we use mice colonized with Citrobacter rodentium or the human γ-Proteobacteria commensal Escherichia coli HS as a model to examine the mechanistic interactions between gut microbes and host responses to cocaine. We find that cocaine exposure increases intestinal norepinephrine levels that are sensed through the bacterial adrenergic receptor QseC to promote intestinal colonization of γ-Proteobacteria. Colonized mice show enhanced host cocaine-induced behaviors. The neuroactive metabolite glycine, a bacterial nitrogen source, is depleted in the gut and cerebrospinal fluid of colonized mice. Systemic glycine repletion reversed, and γ-Proteobacteria mutated for glycine uptake did not alter the host response to cocaine. γ-Proteobacteria modulated glycine levels are linked to cocaine-induced transcriptional plasticity in the nucleus accumbens through glutamatergic transmission. The mechanism outline here could potentially be exploited to modulate reward-related brain circuits that contribute to SUDs.
publishDate 2022
dc.date.none.fl_str_mv 2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213381
Cuesta, Santiago; Burdisso, Paula; Segev, Amir; Kourrich, Saïd; Sperandio, Vanessa; Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses; Cell Press; Cell Host & Microbe; 30; 11; 11-2022; 1615-1629.e5
1931-3128
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213381
identifier_str_mv Cuesta, Santiago; Burdisso, Paula; Segev, Amir; Kourrich, Saïd; Sperandio, Vanessa; Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses; Cell Press; Cell Host & Microbe; 30; 11; 11-2022; 1615-1629.e5
1931-3128
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S193131282200470X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chom.2022.09.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083112477392896
score 12.891075

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