Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation

Autores
Bertucci, Paola Yanina; Nacht, Ana Silvina; Alló, Mariano; Rocha Viegas, Luciana; Ballaré, Cecilia; Soronellas, Daniel; Castellano, Giancarlo; Zaurin, Roser; Kornblihtt, Alberto Rodolfo; Beato, Miguel; Vicent, Guillermo; Pecci, Adali
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of pro- gestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions sur- rounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-dis- tribution of the active Pol II toward the 30-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene ex- pression by facilitating the proper passage of the polymerase along hormone-dependent genes.
Fil: Bertucci, Paola Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Nacht, Ana Silvina. Universitat Pompeu Fabra; España. Centro de Regulación Genómica; España
Fil: Alló, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Rocha Viegas, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Ballaré, Cecilia. Universitat Pompeu Fabra; España. Centro de Regulación Genómica; España
Fil: Soronellas, Daniel. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Castellano, Giancarlo. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Zaurin, Roser. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Kornblihtt, Alberto Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Beato, Miguel. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Vicent, Guillermo. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Pecci, Adali. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
Progesterone
Bcl-X
Polymerase 2
Elongation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2748
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2748
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongationBertucci, Paola YaninaNacht, Ana SilvinaAlló, MarianoRocha Viegas, LucianaBallaré, CeciliaSoronellas, DanielCastellano, GiancarloZaurin, RoserKornblihtt, Alberto RodolfoBeato, MiguelVicent, GuillermoPecci, AdaliProgesteroneBcl-XPolymerase 2Elongationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of pro- gestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions sur- rounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-dis- tribution of the active Pol II toward the 30-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene ex- pression by facilitating the proper passage of the polymerase along hormone-dependent genes.Fil: Bertucci, Paola Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Nacht, Ana Silvina. Universitat Pompeu Fabra; España. Centro de Regulación Genómica; EspañaFil: Alló, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Rocha Viegas, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Ballaré, Cecilia. Universitat Pompeu Fabra; España. Centro de Regulación Genómica; EspañaFil: Soronellas, Daniel. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; EspañaFil: Castellano, Giancarlo. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; EspañaFil: Zaurin, Roser. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; EspañaFil: Kornblihtt, Alberto Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Beato, Miguel. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; EspañaFil: Vicent, Guillermo. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; EspañaFil: Pecci, Adali. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaOxford University Press2013-05-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2748Bertucci, Paola Yanina; Nacht, Ana Silvina; Alló, Mariano; Rocha Viegas, Luciana; Ballaré, Cecilia; et al.; Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation; Oxford University Press; Nucleic Acids Research; 41; 12; 2-5-2013; 6072-60860305-10481362-4962enginfo:eu-repo/semantics/altIdentifier/issn/0305-1048info:eu-repo/semantics/altIdentifier/issn/1362-4962info:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/41/12/6072.longinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695497/info:eu-repo/semantics/altIdentifier/doi/10.1093%2Fnar%2Fgkt327info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:17:11Zoai:ri.conicet.gov.ar:11336/2748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:17:11.841CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
title Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
spellingShingle Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
Bertucci, Paola Yanina
Progesterone
Bcl-X
Polymerase 2
Elongation
title_short Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
title_full Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
title_fullStr Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
title_full_unstemmed Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
title_sort Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation
dc.creator.none.fl_str_mv Bertucci, Paola Yanina
Nacht, Ana Silvina
Alló, Mariano
Rocha Viegas, Luciana
Ballaré, Cecilia
Soronellas, Daniel
Castellano, Giancarlo
Zaurin, Roser
Kornblihtt, Alberto Rodolfo
Beato, Miguel
Vicent, Guillermo
Pecci, Adali
author Bertucci, Paola Yanina
author_facet Bertucci, Paola Yanina
Nacht, Ana Silvina
Alló, Mariano
Rocha Viegas, Luciana
Ballaré, Cecilia
Soronellas, Daniel
Castellano, Giancarlo
Zaurin, Roser
Kornblihtt, Alberto Rodolfo
Beato, Miguel
Vicent, Guillermo
Pecci, Adali
author_role author
author2 Nacht, Ana Silvina
Alló, Mariano
Rocha Viegas, Luciana
Ballaré, Cecilia
Soronellas, Daniel
Castellano, Giancarlo
Zaurin, Roser
Kornblihtt, Alberto Rodolfo
Beato, Miguel
Vicent, Guillermo
Pecci, Adali
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Progesterone
Bcl-X
Polymerase 2
Elongation
topic Progesterone
Bcl-X
Polymerase 2
Elongation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of pro- gestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions sur- rounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-dis- tribution of the active Pol II toward the 30-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene ex- pression by facilitating the proper passage of the polymerase along hormone-dependent genes.
Fil: Bertucci, Paola Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Nacht, Ana Silvina. Universitat Pompeu Fabra; España. Centro de Regulación Genómica; España
Fil: Alló, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Rocha Viegas, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Ballaré, Cecilia. Universitat Pompeu Fabra; España. Centro de Regulación Genómica; España
Fil: Soronellas, Daniel. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Castellano, Giancarlo. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Zaurin, Roser. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Kornblihtt, Alberto Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Beato, Miguel. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Vicent, Guillermo. Centro de Regulación Genómica; España. Universitat Pompeu Fabra; España
Fil: Pecci, Adali. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of pro- gestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions sur- rounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-dis- tribution of the active Pol II toward the 30-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene ex- pression by facilitating the proper passage of the polymerase along hormone-dependent genes.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2748
Bertucci, Paola Yanina; Nacht, Ana Silvina; Alló, Mariano; Rocha Viegas, Luciana; Ballaré, Cecilia; et al.; Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation; Oxford University Press; Nucleic Acids Research; 41; 12; 2-5-2013; 6072-6086
0305-1048
1362-4962
url http://hdl.handle.net/11336/2748
identifier_str_mv Bertucci, Paola Yanina; Nacht, Ana Silvina; Alló, Mariano; Rocha Viegas, Luciana; Ballaré, Cecilia; et al.; Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation; Oxford University Press; Nucleic Acids Research; 41; 12; 2-5-2013; 6072-6086
0305-1048
1362-4962
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0305-1048
info:eu-repo/semantics/altIdentifier/issn/1362-4962
info:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/41/12/6072.long
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695497/
info:eu-repo/semantics/altIdentifier/doi/10.1093%2Fnar%2Fgkt327
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.229304

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