The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
- Autores
- Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; Sanchez, Daniel Oscar
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation.
Fil: Bañuelos, Carolina Paula. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Levy, Gabriela Vanesa. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Níttolo, Analía Gabriela. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Roser, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina - Materia
-
CHROMATIN REMODELING
EPIGENETIC REGULATION
RNA POLYMERASE II
TRANS-SPLICING
TRANSCRIPTION ELONGATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/151821
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The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genesBañuelos, Carolina PaulaLevy, Gabriela VanesaNíttolo, Analía GabrielaRoser, LeandroTekiel, Valeria SoniaSanchez, Daniel OscarCHROMATIN REMODELINGEPIGENETIC REGULATIONRNA POLYMERASE IITRANS-SPLICINGTRANSCRIPTION ELONGATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation.Fil: Bañuelos, Carolina Paula. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Levy, Gabriela Vanesa. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Níttolo, Analía Gabriela. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Roser, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaWiley Blackwell Publishing, Inc2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151821Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; et al.; The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 66; 5; 9-2019; 719-7291066-52341550-7408CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12716info:eu-repo/semantics/altIdentifier/doi/10.1111/jeu.12716info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:17:11Zoai:ri.conicet.gov.ar:11336/151821instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:17:11.698CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes |
title |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes |
spellingShingle |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes Bañuelos, Carolina Paula CHROMATIN REMODELING EPIGENETIC REGULATION RNA POLYMERASE II TRANS-SPLICING TRANSCRIPTION ELONGATION |
title_short |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes |
title_full |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes |
title_fullStr |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes |
title_full_unstemmed |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes |
title_sort |
The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes |
dc.creator.none.fl_str_mv |
Bañuelos, Carolina Paula Levy, Gabriela Vanesa Níttolo, Analía Gabriela Roser, Leandro Tekiel, Valeria Sonia Sanchez, Daniel Oscar |
author |
Bañuelos, Carolina Paula |
author_facet |
Bañuelos, Carolina Paula Levy, Gabriela Vanesa Níttolo, Analía Gabriela Roser, Leandro Tekiel, Valeria Sonia Sanchez, Daniel Oscar |
author_role |
author |
author2 |
Levy, Gabriela Vanesa Níttolo, Analía Gabriela Roser, Leandro Tekiel, Valeria Sonia Sanchez, Daniel Oscar |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CHROMATIN REMODELING EPIGENETIC REGULATION RNA POLYMERASE II TRANS-SPLICING TRANSCRIPTION ELONGATION |
topic |
CHROMATIN REMODELING EPIGENETIC REGULATION RNA POLYMERASE II TRANS-SPLICING TRANSCRIPTION ELONGATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation. Fil: Bañuelos, Carolina Paula. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Levy, Gabriela Vanesa. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Níttolo, Analía Gabriela. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Roser, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina |
description |
It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/151821 Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; et al.; The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 66; 5; 9-2019; 719-729 1066-5234 1550-7408 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/151821 |
identifier_str_mv |
Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; et al.; The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 66; 5; 9-2019; 719-729 1066-5234 1550-7408 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12716 info:eu-repo/semantics/altIdentifier/doi/10.1111/jeu.12716 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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12.982451 |