The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes

Autores
Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; Sanchez, Daniel Oscar
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation.
Fil: Bañuelos, Carolina Paula. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Levy, Gabriela Vanesa. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Níttolo, Analía Gabriela. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Roser, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
CHROMATIN REMODELING
EPIGENETIC REGULATION
RNA POLYMERASE II
TRANS-SPLICING
TRANSCRIPTION ELONGATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151821

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network_name_str CONICET Digital (CONICET)
spelling The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genesBañuelos, Carolina PaulaLevy, Gabriela VanesaNíttolo, Analía GabrielaRoser, LeandroTekiel, Valeria SoniaSanchez, Daniel OscarCHROMATIN REMODELINGEPIGENETIC REGULATIONRNA POLYMERASE IITRANS-SPLICINGTRANSCRIPTION ELONGATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation.Fil: Bañuelos, Carolina Paula. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Levy, Gabriela Vanesa. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Níttolo, Analía Gabriela. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Roser, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaWiley Blackwell Publishing, Inc2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151821Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; et al.; The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 66; 5; 9-2019; 719-7291066-52341550-7408CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12716info:eu-repo/semantics/altIdentifier/doi/10.1111/jeu.12716info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:17:11Zoai:ri.conicet.gov.ar:11336/151821instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:17:11.698CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
title The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
spellingShingle The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
Bañuelos, Carolina Paula
CHROMATIN REMODELING
EPIGENETIC REGULATION
RNA POLYMERASE II
TRANS-SPLICING
TRANSCRIPTION ELONGATION
title_short The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
title_full The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
title_fullStr The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
title_full_unstemmed The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
title_sort The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes
dc.creator.none.fl_str_mv Bañuelos, Carolina Paula
Levy, Gabriela Vanesa
Níttolo, Analía Gabriela
Roser, Leandro
Tekiel, Valeria Sonia
Sanchez, Daniel Oscar
author Bañuelos, Carolina Paula
author_facet Bañuelos, Carolina Paula
Levy, Gabriela Vanesa
Níttolo, Analía Gabriela
Roser, Leandro
Tekiel, Valeria Sonia
Sanchez, Daniel Oscar
author_role author
author2 Levy, Gabriela Vanesa
Níttolo, Analía Gabriela
Roser, Leandro
Tekiel, Valeria Sonia
Sanchez, Daniel Oscar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CHROMATIN REMODELING
EPIGENETIC REGULATION
RNA POLYMERASE II
TRANS-SPLICING
TRANSCRIPTION ELONGATION
topic CHROMATIN REMODELING
EPIGENETIC REGULATION
RNA POLYMERASE II
TRANS-SPLICING
TRANSCRIPTION ELONGATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation.
Fil: Bañuelos, Carolina Paula. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Levy, Gabriela Vanesa. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Níttolo, Analía Gabriela. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Roser, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
description It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151821
Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; et al.; The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 66; 5; 9-2019; 719-729
1066-5234
1550-7408
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151821
identifier_str_mv Bañuelos, Carolina Paula; Levy, Gabriela Vanesa; Níttolo, Analía Gabriela; Roser, Leandro; Tekiel, Valeria Sonia; et al.; The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 66; 5; 9-2019; 719-729
1066-5234
1550-7408
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12716
info:eu-repo/semantics/altIdentifier/doi/10.1111/jeu.12716
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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