Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse

Autores
Miao, Yi Liang; Gambini, Andres; Zhang, Yingpei; Padilla Banks, Elizabeth; Jefferson, Wendy N.; Bernhardt, Miranda L.; Huang, Weichun; Li, Leping; Williams, Carmen J.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding factors that regulate zygotic genome activation (ZGA) is critical for determining how cells are reprogrammed to become totipotent or pluripotent. There is limited information regarding how this process occurs physiologically in early mammalian embryos. Here, we identify a mediator complex subunit, MED13, as translated during mouse oocyte maturation and transcribed early from the zygotic genome. Knockdown and conditional knockout approaches demonstrate that MED13 is essential for ZGA in the mouse, in part by regulating expression of the embryo-specific chromatin remodeling complex, esBAF. The role of MED13 in ZGA is mediated in part by interactions with E2F transcription factors. In addition to MED13, its paralog, MED13L, is required for successful preimplantation embryo development. MED13L partially compensates for loss of MED13 function in preimplantation knockout embryos, but postimplantation development is not rescued by MED13L. Our data demonstrate an essential role for MED13 in supporting chromatin reprogramming and directed transcription of essential genes during ZGA.
Fil: Miao, Yi Liang. National Institutes of Health; Estados Unidos
Fil: Gambini, Andres. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zhang, Yingpei. National Institutes of Health; Estados Unidos
Fil: Padilla Banks, Elizabeth. National Institutes of Health; Estados Unidos
Fil: Jefferson, Wendy N.. National Institutes of Health; Estados Unidos
Fil: Bernhardt, Miranda L.. National Institutes of Health; Estados Unidos
Fil: Huang, Weichun. National Institutes of Health; Estados Unidos
Fil: Li, Leping. National Institutes of Health; Estados Unidos
Fil: Williams, Carmen J.. National Institutes of Health; Estados Unidos
Materia
MED13
MED13L
MEDIATOR COMPLEX
OOCYTE-TO-EMBRYO TRANSITION
PREIMPLANTATION EMBRYO
TRANSCRIPTION
TRANSLATION
ZYGOTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96205
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96205
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouseMiao, Yi LiangGambini, AndresZhang, YingpeiPadilla Banks, ElizabethJefferson, Wendy N.Bernhardt, Miranda L.Huang, WeichunLi, LepingWilliams, Carmen J.MED13MED13LMEDIATOR COMPLEXOOCYTE-TO-EMBRYO TRANSITIONPREIMPLANTATION EMBRYOTRANSCRIPTIONTRANSLATIONZYGOTEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Understanding factors that regulate zygotic genome activation (ZGA) is critical for determining how cells are reprogrammed to become totipotent or pluripotent. There is limited information regarding how this process occurs physiologically in early mammalian embryos. Here, we identify a mediator complex subunit, MED13, as translated during mouse oocyte maturation and transcribed early from the zygotic genome. Knockdown and conditional knockout approaches demonstrate that MED13 is essential for ZGA in the mouse, in part by regulating expression of the embryo-specific chromatin remodeling complex, esBAF. The role of MED13 in ZGA is mediated in part by interactions with E2F transcription factors. In addition to MED13, its paralog, MED13L, is required for successful preimplantation embryo development. MED13L partially compensates for loss of MED13 function in preimplantation knockout embryos, but postimplantation development is not rescued by MED13L. Our data demonstrate an essential role for MED13 in supporting chromatin reprogramming and directed transcription of essential genes during ZGA.Fil: Miao, Yi Liang. National Institutes of Health; Estados UnidosFil: Gambini, Andres. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zhang, Yingpei. National Institutes of Health; Estados UnidosFil: Padilla Banks, Elizabeth. National Institutes of Health; Estados UnidosFil: Jefferson, Wendy N.. National Institutes of Health; Estados UnidosFil: Bernhardt, Miranda L.. National Institutes of Health; Estados UnidosFil: Huang, Weichun. National Institutes of Health; Estados UnidosFil: Li, Leping. National Institutes of Health; Estados UnidosFil: Williams, Carmen J.. National Institutes of Health; Estados UnidosOxford University Press2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96205Miao, Yi Liang; Gambini, Andres; Zhang, Yingpei; Padilla Banks, Elizabeth; Jefferson, Wendy N.; et al.; Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse; Oxford University Press; Biology of Reproduction; 98; 4; 4-2018; 449-4640006-3363CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/98/4/449/4794936info:eu-repo/semantics/altIdentifier/doi/10.1093/biolre/ioy004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:44Zoai:ri.conicet.gov.ar:11336/96205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:44.964CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
title Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
spellingShingle Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
Miao, Yi Liang
MED13
MED13L
MEDIATOR COMPLEX
OOCYTE-TO-EMBRYO TRANSITION
PREIMPLANTATION EMBRYO
TRANSCRIPTION
TRANSLATION
ZYGOTE
title_short Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
title_full Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
title_fullStr Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
title_full_unstemmed Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
title_sort Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
dc.creator.none.fl_str_mv Miao, Yi Liang
Gambini, Andres
Zhang, Yingpei
Padilla Banks, Elizabeth
Jefferson, Wendy N.
Bernhardt, Miranda L.
Huang, Weichun
Li, Leping
Williams, Carmen J.
author Miao, Yi Liang
author_facet Miao, Yi Liang
Gambini, Andres
Zhang, Yingpei
Padilla Banks, Elizabeth
Jefferson, Wendy N.
Bernhardt, Miranda L.
Huang, Weichun
Li, Leping
Williams, Carmen J.
author_role author
author2 Gambini, Andres
Zhang, Yingpei
Padilla Banks, Elizabeth
Jefferson, Wendy N.
Bernhardt, Miranda L.
Huang, Weichun
Li, Leping
Williams, Carmen J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MED13
MED13L
MEDIATOR COMPLEX
OOCYTE-TO-EMBRYO TRANSITION
PREIMPLANTATION EMBRYO
TRANSCRIPTION
TRANSLATION
ZYGOTE
topic MED13
MED13L
MEDIATOR COMPLEX
OOCYTE-TO-EMBRYO TRANSITION
PREIMPLANTATION EMBRYO
TRANSCRIPTION
TRANSLATION
ZYGOTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Understanding factors that regulate zygotic genome activation (ZGA) is critical for determining how cells are reprogrammed to become totipotent or pluripotent. There is limited information regarding how this process occurs physiologically in early mammalian embryos. Here, we identify a mediator complex subunit, MED13, as translated during mouse oocyte maturation and transcribed early from the zygotic genome. Knockdown and conditional knockout approaches demonstrate that MED13 is essential for ZGA in the mouse, in part by regulating expression of the embryo-specific chromatin remodeling complex, esBAF. The role of MED13 in ZGA is mediated in part by interactions with E2F transcription factors. In addition to MED13, its paralog, MED13L, is required for successful preimplantation embryo development. MED13L partially compensates for loss of MED13 function in preimplantation knockout embryos, but postimplantation development is not rescued by MED13L. Our data demonstrate an essential role for MED13 in supporting chromatin reprogramming and directed transcription of essential genes during ZGA.
Fil: Miao, Yi Liang. National Institutes of Health; Estados Unidos
Fil: Gambini, Andres. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zhang, Yingpei. National Institutes of Health; Estados Unidos
Fil: Padilla Banks, Elizabeth. National Institutes of Health; Estados Unidos
Fil: Jefferson, Wendy N.. National Institutes of Health; Estados Unidos
Fil: Bernhardt, Miranda L.. National Institutes of Health; Estados Unidos
Fil: Huang, Weichun. National Institutes of Health; Estados Unidos
Fil: Li, Leping. National Institutes of Health; Estados Unidos
Fil: Williams, Carmen J.. National Institutes of Health; Estados Unidos
description Understanding factors that regulate zygotic genome activation (ZGA) is critical for determining how cells are reprogrammed to become totipotent or pluripotent. There is limited information regarding how this process occurs physiologically in early mammalian embryos. Here, we identify a mediator complex subunit, MED13, as translated during mouse oocyte maturation and transcribed early from the zygotic genome. Knockdown and conditional knockout approaches demonstrate that MED13 is essential for ZGA in the mouse, in part by regulating expression of the embryo-specific chromatin remodeling complex, esBAF. The role of MED13 in ZGA is mediated in part by interactions with E2F transcription factors. In addition to MED13, its paralog, MED13L, is required for successful preimplantation embryo development. MED13L partially compensates for loss of MED13 function in preimplantation knockout embryos, but postimplantation development is not rescued by MED13L. Our data demonstrate an essential role for MED13 in supporting chromatin reprogramming and directed transcription of essential genes during ZGA.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96205
Miao, Yi Liang; Gambini, Andres; Zhang, Yingpei; Padilla Banks, Elizabeth; Jefferson, Wendy N.; et al.; Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse; Oxford University Press; Biology of Reproduction; 98; 4; 4-2018; 449-464
0006-3363
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96205
identifier_str_mv Miao, Yi Liang; Gambini, Andres; Zhang, Yingpei; Padilla Banks, Elizabeth; Jefferson, Wendy N.; et al.; Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse; Oxford University Press; Biology of Reproduction; 98; 4; 4-2018; 449-464
0006-3363
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/98/4/449/4794936
info:eu-repo/semantics/altIdentifier/doi/10.1093/biolre/ioy004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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