Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos

Autores
Campello, Iana S.; Pereira, Alexsandra F.; Alcântara Neto, Agostinho S.; Canel, Natalia G.; Souza Fabjan, Joanna M. G.; Teixeira, Dárcio I. A.; Camargo, Luiz S. A.; Melo, Luciana M.; Rádis Baptista, Gandhi; Salamone, Daniel Felipe; Freitas, Vicente J. F.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study investigated the effects of crotamine, a cell-penetrating peptide from rattlesnake venom, at different exposure times and concentrations, on both developmental competence and gene expression (ATP1A1, AQP3, GLUT1 and GLUT3) of in vitro fertilized (IVF) bovine embryos. In Experiment 1, presumptive zygotes were exposed to 0.1 μM crotamine for 6, 12 or 24 h and control groups (vehicle and IVF) were included. In Experiment 2, presumptive zygotes were exposed to 0 (vehicle), 0.1, 1 and 10 μM crotamine for 24 h. Additionally, to visualize crotamine uptake, embryos were exposed to rhodamine B-labelled crotamine and subjected to confocal microscopy. In Experiment 1, no difference (P > 0.05) was observed among different exposure times and control groups for cleavage and blastocyst rates and total cells number per blastocyst. Within each exposure time, mRNA levels were similar (P > 0.05) in embryos cultured with or without crotamine. In Experiment 2, concentrations as high as 10 μM crotamine did not affect (P > 0.05) the blastocyst rate. Crotamine at 0.1 and 10 μM did not alter mRNA levels when compared with the control (P > 0.05). Remarkably, only 1 μM crotamine decreased both ATP1A1 and AQP3 expression levels relative to the control group (P < 0.05). Also, it was possible to visualize the intracellular localization of crotamine. These results indicate that crotamine can translocate intact IVF bovine embryos and its application in the culture medium is possible at concentrations from 0.1–10 μM for 6–24 h.
Fil: Campello, Iana S.. Universidade Estadual Do Ceara; Brasil
Fil: Pereira, Alexsandra F.. Universidade Estadual Do Ceara; Brasil
Fil: Alcântara Neto, Agostinho S.. Universidade Estadual Do Ceara; Brasil
Fil: Canel, Natalia G.. Universidad de Buenos Aires; Argentina
Fil: Souza Fabjan, Joanna M. G.. Universidade Estadual Do Ceara; Brasil
Fil: Teixeira, Dárcio I. A.. Universidade Estadual Do Ceara; Brasil
Fil: Camargo, Luiz S. A.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Melo, Luciana M.. Universidade Estadual Do Ceara; Brasil
Fil: Rádis Baptista, Gandhi. Universidade Federal do Ceará; Brasil
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freitas, Vicente J. F.. Universidade Estadual Do Ceara; Brasil
Materia
Crotamina
Preimplantation Embryo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30206
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30206
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryosCampello, Iana S.Pereira, Alexsandra F.Alcântara Neto, Agostinho S.Canel, Natalia G.Souza Fabjan, Joanna M. G.Teixeira, Dárcio I. A.Camargo, Luiz S. A.Melo, Luciana M.Rádis Baptista, GandhiSalamone, Daniel FelipeFreitas, Vicente J. F.CrotaminaPreimplantation Embryohttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The present study investigated the effects of crotamine, a cell-penetrating peptide from rattlesnake venom, at different exposure times and concentrations, on both developmental competence and gene expression (ATP1A1, AQP3, GLUT1 and GLUT3) of in vitro fertilized (IVF) bovine embryos. In Experiment 1, presumptive zygotes were exposed to 0.1 μM crotamine for 6, 12 or 24 h and control groups (vehicle and IVF) were included. In Experiment 2, presumptive zygotes were exposed to 0 (vehicle), 0.1, 1 and 10 μM crotamine for 24 h. Additionally, to visualize crotamine uptake, embryos were exposed to rhodamine B-labelled crotamine and subjected to confocal microscopy. In Experiment 1, no difference (P > 0.05) was observed among different exposure times and control groups for cleavage and blastocyst rates and total cells number per blastocyst. Within each exposure time, mRNA levels were similar (P > 0.05) in embryos cultured with or without crotamine. In Experiment 2, concentrations as high as 10 μM crotamine did not affect (P > 0.05) the blastocyst rate. Crotamine at 0.1 and 10 μM did not alter mRNA levels when compared with the control (P > 0.05). Remarkably, only 1 μM crotamine decreased both ATP1A1 and AQP3 expression levels relative to the control group (P < 0.05). Also, it was possible to visualize the intracellular localization of crotamine. These results indicate that crotamine can translocate intact IVF bovine embryos and its application in the culture medium is possible at concentrations from 0.1–10 μM for 6–24 h.Fil: Campello, Iana S.. Universidade Estadual Do Ceara; BrasilFil: Pereira, Alexsandra F.. Universidade Estadual Do Ceara; BrasilFil: Alcântara Neto, Agostinho S.. Universidade Estadual Do Ceara; BrasilFil: Canel, Natalia G.. Universidad de Buenos Aires; ArgentinaFil: Souza Fabjan, Joanna M. G.. Universidade Estadual Do Ceara; BrasilFil: Teixeira, Dárcio I. A.. Universidade Estadual Do Ceara; BrasilFil: Camargo, Luiz S. A.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Melo, Luciana M.. Universidade Estadual Do Ceara; BrasilFil: Rádis Baptista, Gandhi. Universidade Federal do Ceará; BrasilFil: Salamone, Daniel Felipe. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Freitas, Vicente J. F.. Universidade Estadual Do Ceara; BrasilCambridge University Press2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30206Campello, Iana S.; Pereira, Alexsandra F.; Alcântara Neto, Agostinho S.; Canel, Natalia G. ; Souza Fabjan, Joanna M. G.; et al.; Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos; Cambridge University Press; Zygote; 24; 1; 12-2014; 48-570967-1994CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199414000707info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/effect-of-crotamine-a-cellpenetrating-peptide-on-blastocyst-production-and-gene-expression-of-in-vitro-fertilized-bovine-embryos/4DFCDCA8AEB3D413F9DD445FA1B638C5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:40Zoai:ri.conicet.gov.ar:11336/30206instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:40.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
title Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
spellingShingle Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
Campello, Iana S.
Crotamina
Preimplantation Embryo
title_short Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
title_full Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
title_fullStr Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
title_full_unstemmed Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
title_sort Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos
dc.creator.none.fl_str_mv Campello, Iana S.
Pereira, Alexsandra F.
Alcântara Neto, Agostinho S.
Canel, Natalia G.
Souza Fabjan, Joanna M. G.
Teixeira, Dárcio I. A.
Camargo, Luiz S. A.
Melo, Luciana M.
Rádis Baptista, Gandhi
Salamone, Daniel Felipe
Freitas, Vicente J. F.
author Campello, Iana S.
author_facet Campello, Iana S.
Pereira, Alexsandra F.
Alcântara Neto, Agostinho S.
Canel, Natalia G.
Souza Fabjan, Joanna M. G.
Teixeira, Dárcio I. A.
Camargo, Luiz S. A.
Melo, Luciana M.
Rádis Baptista, Gandhi
Salamone, Daniel Felipe
Freitas, Vicente J. F.
author_role author
author2 Pereira, Alexsandra F.
Alcântara Neto, Agostinho S.
Canel, Natalia G.
Souza Fabjan, Joanna M. G.
Teixeira, Dárcio I. A.
Camargo, Luiz S. A.
Melo, Luciana M.
Rádis Baptista, Gandhi
Salamone, Daniel Felipe
Freitas, Vicente J. F.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Crotamina
Preimplantation Embryo
topic Crotamina
Preimplantation Embryo
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The present study investigated the effects of crotamine, a cell-penetrating peptide from rattlesnake venom, at different exposure times and concentrations, on both developmental competence and gene expression (ATP1A1, AQP3, GLUT1 and GLUT3) of in vitro fertilized (IVF) bovine embryos. In Experiment 1, presumptive zygotes were exposed to 0.1 μM crotamine for 6, 12 or 24 h and control groups (vehicle and IVF) were included. In Experiment 2, presumptive zygotes were exposed to 0 (vehicle), 0.1, 1 and 10 μM crotamine for 24 h. Additionally, to visualize crotamine uptake, embryos were exposed to rhodamine B-labelled crotamine and subjected to confocal microscopy. In Experiment 1, no difference (P > 0.05) was observed among different exposure times and control groups for cleavage and blastocyst rates and total cells number per blastocyst. Within each exposure time, mRNA levels were similar (P > 0.05) in embryos cultured with or without crotamine. In Experiment 2, concentrations as high as 10 μM crotamine did not affect (P > 0.05) the blastocyst rate. Crotamine at 0.1 and 10 μM did not alter mRNA levels when compared with the control (P > 0.05). Remarkably, only 1 μM crotamine decreased both ATP1A1 and AQP3 expression levels relative to the control group (P < 0.05). Also, it was possible to visualize the intracellular localization of crotamine. These results indicate that crotamine can translocate intact IVF bovine embryos and its application in the culture medium is possible at concentrations from 0.1–10 μM for 6–24 h.
Fil: Campello, Iana S.. Universidade Estadual Do Ceara; Brasil
Fil: Pereira, Alexsandra F.. Universidade Estadual Do Ceara; Brasil
Fil: Alcântara Neto, Agostinho S.. Universidade Estadual Do Ceara; Brasil
Fil: Canel, Natalia G.. Universidad de Buenos Aires; Argentina
Fil: Souza Fabjan, Joanna M. G.. Universidade Estadual Do Ceara; Brasil
Fil: Teixeira, Dárcio I. A.. Universidade Estadual Do Ceara; Brasil
Fil: Camargo, Luiz S. A.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Melo, Luciana M.. Universidade Estadual Do Ceara; Brasil
Fil: Rádis Baptista, Gandhi. Universidade Federal do Ceará; Brasil
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freitas, Vicente J. F.. Universidade Estadual Do Ceara; Brasil
description The present study investigated the effects of crotamine, a cell-penetrating peptide from rattlesnake venom, at different exposure times and concentrations, on both developmental competence and gene expression (ATP1A1, AQP3, GLUT1 and GLUT3) of in vitro fertilized (IVF) bovine embryos. In Experiment 1, presumptive zygotes were exposed to 0.1 μM crotamine for 6, 12 or 24 h and control groups (vehicle and IVF) were included. In Experiment 2, presumptive zygotes were exposed to 0 (vehicle), 0.1, 1 and 10 μM crotamine for 24 h. Additionally, to visualize crotamine uptake, embryos were exposed to rhodamine B-labelled crotamine and subjected to confocal microscopy. In Experiment 1, no difference (P > 0.05) was observed among different exposure times and control groups for cleavage and blastocyst rates and total cells number per blastocyst. Within each exposure time, mRNA levels were similar (P > 0.05) in embryos cultured with or without crotamine. In Experiment 2, concentrations as high as 10 μM crotamine did not affect (P > 0.05) the blastocyst rate. Crotamine at 0.1 and 10 μM did not alter mRNA levels when compared with the control (P > 0.05). Remarkably, only 1 μM crotamine decreased both ATP1A1 and AQP3 expression levels relative to the control group (P < 0.05). Also, it was possible to visualize the intracellular localization of crotamine. These results indicate that crotamine can translocate intact IVF bovine embryos and its application in the culture medium is possible at concentrations from 0.1–10 μM for 6–24 h.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30206
Campello, Iana S.; Pereira, Alexsandra F.; Alcântara Neto, Agostinho S.; Canel, Natalia G. ; Souza Fabjan, Joanna M. G.; et al.; Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos; Cambridge University Press; Zygote; 24; 1; 12-2014; 48-57
0967-1994
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30206
identifier_str_mv Campello, Iana S.; Pereira, Alexsandra F.; Alcântara Neto, Agostinho S.; Canel, Natalia G. ; Souza Fabjan, Joanna M. G.; et al.; Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos; Cambridge University Press; Zygote; 24; 1; 12-2014; 48-57
0967-1994
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199414000707
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/effect-of-crotamine-a-cellpenetrating-peptide-on-blastocyst-production-and-gene-expression-of-in-vitro-fertilized-bovine-embryos/4DFCDCA8AEB3D413F9DD445FA1B638C5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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