The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans

Autores
Cloutier, Monikca; Castilla Lozano, Maria del Rocio; Bolduc, Nathalie; Zelada, Alicia Mercedes; Martineau, Philippe; Bouillon, Marlène; Magee, Beatrice B.; Di Bernardo, Maria Susana; Giasson, Luc; Cantore, Maria Leonor
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have cloned the Candida albicans TPK2 gene encoding a cAMP-dependent protein kinase (PKA) catalytic subunit and generated a tpk2 homozygous null mutant to assess its ability to germinate in liquid media. N-acetylglucosamine (GlcNAc)-induced germ-tube formation was attenuated in the tpk2 strain and enhanced by compounds that are known to increase the PKA activity in situ. Germination was completely blocked in the presence of the myristoylated derivative of the heat-stable PKA inhibitor (MyrPKI). These results indicate that TPK1 acts positively in regulating the morphogenetic transition in C. albicans in the absence of the TPK2 gene. We were able to identify an mRNA from this second form of PKA in both wild-type and tpk2 null mutant cells. We found that PKA activity measured in the mutant lacking the TPK2 gene was about 10% of that displayed by the wild-type. The finding that the germinative response of tpk2 null mutant to serum was severely diminished at low serum concentrations indicates that the level of PKA is an important determinant of filamentous growth at low serum concentrations. The extent of germination attained at higher serum concentrations (5%) was similar in the wild-type and in the tpk2 null mutant strains suggesting that under these conditions germination was triggered through a PKA-independent pathway.
Fil: Cloutier, Monikca. GREB Laval University; Canadá
Fil: Castilla Lozano, Maria del Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Bolduc, Nathalie. GREB Laval University; Canadá
Fil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Martineau, Philippe. GREB Laval University; Canadá
Fil: Bouillon, Marlène. GREB Laval University; Canadá
Fil: Magee, Beatrice B.. University of Minnesota; Estados Unidos
Fil: Di Bernardo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Giasson, Luc. GREB Laval University; Canadá
Fil: Cantore, Maria Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Materia
Camp
Camp-Dependent Protein Kinase
Candida Albicans
Morphogenesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79778
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79778
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicansCloutier, MonikcaCastilla Lozano, Maria del RocioBolduc, NathalieZelada, Alicia MercedesMartineau, PhilippeBouillon, MarlèneMagee, Beatrice B.Di Bernardo, Maria SusanaGiasson, LucCantore, Maria LeonorCampCamp-Dependent Protein KinaseCandida AlbicansMorphogenesishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have cloned the Candida albicans TPK2 gene encoding a cAMP-dependent protein kinase (PKA) catalytic subunit and generated a tpk2 homozygous null mutant to assess its ability to germinate in liquid media. N-acetylglucosamine (GlcNAc)-induced germ-tube formation was attenuated in the tpk2 strain and enhanced by compounds that are known to increase the PKA activity in situ. Germination was completely blocked in the presence of the myristoylated derivative of the heat-stable PKA inhibitor (MyrPKI). These results indicate that TPK1 acts positively in regulating the morphogenetic transition in C. albicans in the absence of the TPK2 gene. We were able to identify an mRNA from this second form of PKA in both wild-type and tpk2 null mutant cells. We found that PKA activity measured in the mutant lacking the TPK2 gene was about 10% of that displayed by the wild-type. The finding that the germinative response of tpk2 null mutant to serum was severely diminished at low serum concentrations indicates that the level of PKA is an important determinant of filamentous growth at low serum concentrations. The extent of germination attained at higher serum concentrations (5%) was similar in the wild-type and in the tpk2 null mutant strains suggesting that under these conditions germination was triggered through a PKA-independent pathway.Fil: Cloutier, Monikca. GREB Laval University; CanadáFil: Castilla Lozano, Maria del Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaFil: Bolduc, Nathalie. GREB Laval University; CanadáFil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaFil: Martineau, Philippe. GREB Laval University; CanadáFil: Bouillon, Marlène. GREB Laval University; CanadáFil: Magee, Beatrice B.. University of Minnesota; Estados UnidosFil: Di Bernardo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaFil: Giasson, Luc. GREB Laval University; CanadáFil: Cantore, Maria Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaAcademic Press Inc Elsevier Science2003-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79778Cloutier, Monikca; Castilla Lozano, Maria del Rocio; Bolduc, Nathalie; Zelada, Alicia Mercedes; Martineau, Philippe; et al.; The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 38; 1; 2-2003; 133-1411087-1845CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12553943info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1087184502005200info:eu-repo/semantics/altIdentifier/doi/10.1016/S1087-1845(02)00520-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:03:23Zoai:ri.conicet.gov.ar:11336/79778instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:03:23.345CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
title The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
spellingShingle The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
Cloutier, Monikca
Camp
Camp-Dependent Protein Kinase
Candida Albicans
Morphogenesis
title_short The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
title_full The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
title_fullStr The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
title_full_unstemmed The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
title_sort The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans
dc.creator.none.fl_str_mv Cloutier, Monikca
Castilla Lozano, Maria del Rocio
Bolduc, Nathalie
Zelada, Alicia Mercedes
Martineau, Philippe
Bouillon, Marlène
Magee, Beatrice B.
Di Bernardo, Maria Susana
Giasson, Luc
Cantore, Maria Leonor
author Cloutier, Monikca
author_facet Cloutier, Monikca
Castilla Lozano, Maria del Rocio
Bolduc, Nathalie
Zelada, Alicia Mercedes
Martineau, Philippe
Bouillon, Marlène
Magee, Beatrice B.
Di Bernardo, Maria Susana
Giasson, Luc
Cantore, Maria Leonor
author_role author
author2 Castilla Lozano, Maria del Rocio
Bolduc, Nathalie
Zelada, Alicia Mercedes
Martineau, Philippe
Bouillon, Marlène
Magee, Beatrice B.
Di Bernardo, Maria Susana
Giasson, Luc
Cantore, Maria Leonor
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Camp
Camp-Dependent Protein Kinase
Candida Albicans
Morphogenesis
topic Camp
Camp-Dependent Protein Kinase
Candida Albicans
Morphogenesis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have cloned the Candida albicans TPK2 gene encoding a cAMP-dependent protein kinase (PKA) catalytic subunit and generated a tpk2 homozygous null mutant to assess its ability to germinate in liquid media. N-acetylglucosamine (GlcNAc)-induced germ-tube formation was attenuated in the tpk2 strain and enhanced by compounds that are known to increase the PKA activity in situ. Germination was completely blocked in the presence of the myristoylated derivative of the heat-stable PKA inhibitor (MyrPKI). These results indicate that TPK1 acts positively in regulating the morphogenetic transition in C. albicans in the absence of the TPK2 gene. We were able to identify an mRNA from this second form of PKA in both wild-type and tpk2 null mutant cells. We found that PKA activity measured in the mutant lacking the TPK2 gene was about 10% of that displayed by the wild-type. The finding that the germinative response of tpk2 null mutant to serum was severely diminished at low serum concentrations indicates that the level of PKA is an important determinant of filamentous growth at low serum concentrations. The extent of germination attained at higher serum concentrations (5%) was similar in the wild-type and in the tpk2 null mutant strains suggesting that under these conditions germination was triggered through a PKA-independent pathway.
Fil: Cloutier, Monikca. GREB Laval University; Canadá
Fil: Castilla Lozano, Maria del Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Bolduc, Nathalie. GREB Laval University; Canadá
Fil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Martineau, Philippe. GREB Laval University; Canadá
Fil: Bouillon, Marlène. GREB Laval University; Canadá
Fil: Magee, Beatrice B.. University of Minnesota; Estados Unidos
Fil: Di Bernardo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Giasson, Luc. GREB Laval University; Canadá
Fil: Cantore, Maria Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
description We have cloned the Candida albicans TPK2 gene encoding a cAMP-dependent protein kinase (PKA) catalytic subunit and generated a tpk2 homozygous null mutant to assess its ability to germinate in liquid media. N-acetylglucosamine (GlcNAc)-induced germ-tube formation was attenuated in the tpk2 strain and enhanced by compounds that are known to increase the PKA activity in situ. Germination was completely blocked in the presence of the myristoylated derivative of the heat-stable PKA inhibitor (MyrPKI). These results indicate that TPK1 acts positively in regulating the morphogenetic transition in C. albicans in the absence of the TPK2 gene. We were able to identify an mRNA from this second form of PKA in both wild-type and tpk2 null mutant cells. We found that PKA activity measured in the mutant lacking the TPK2 gene was about 10% of that displayed by the wild-type. The finding that the germinative response of tpk2 null mutant to serum was severely diminished at low serum concentrations indicates that the level of PKA is an important determinant of filamentous growth at low serum concentrations. The extent of germination attained at higher serum concentrations (5%) was similar in the wild-type and in the tpk2 null mutant strains suggesting that under these conditions germination was triggered through a PKA-independent pathway.
publishDate 2003
dc.date.none.fl_str_mv 2003-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79778
Cloutier, Monikca; Castilla Lozano, Maria del Rocio; Bolduc, Nathalie; Zelada, Alicia Mercedes; Martineau, Philippe; et al.; The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 38; 1; 2-2003; 133-141
1087-1845
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79778
identifier_str_mv Cloutier, Monikca; Castilla Lozano, Maria del Rocio; Bolduc, Nathalie; Zelada, Alicia Mercedes; Martineau, Philippe; et al.; The two isoforms of the cAMP-dependent protein kinase catalytic subunit are involved in the control of dimorphism in the human fungal pathogen Candida albicans; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 38; 1; 2-2003; 133-141
1087-1845
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12553943
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1087184502005200
info:eu-repo/semantics/altIdentifier/doi/10.1016/S1087-1845(02)00520-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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