Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth

Autores
Cesari, Adriana Belen; Fernandez, Marilina; Paulucci, Natalia Soledad; Dardanelli, Marta Susana
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Currently, bacterial inoculant technology focuses on improving long-term storage conditionsto ensure adequate rhizobia numbers and their effectiveness as plant growth promoters. Thisstudy aimed to investigate whether storage at 4 ◦C for four years of alginate beads immobilizingBradyrhizobium sp. SEMIA6144 maintains bacterial vitality, efficacy in growth promotion, and abilityto establish early interactions with Arachis hypogaea L. The recovery of viable SEMIA6144 cellsdecreased over time (10% at six months, 1% at one year, and 0.01% at four years), while cell vitalityremained high at 94.1%, 90.2%, and 93.4%, respectively. The unsaturated/saturated fatty acid ratiodeclined during storage, reducing membrane fluidity and metabolic activity. Mobility and rootadhesion of SEMIA6144 decreased after one and four years. However, growth promotion in peanutsinoculated with SEMIA6144 beads was observed through increased biomass, total chlorophyll, leafnumber, leaf area, and decreased chlorophyll fluorescence compared to non-inoculated plants. Althoughnodulation was low in plants inoculated with four-year-old beads, leghemoglobin levelswere maintained. These results demonstrate that Bradyrhizobium sp. SEMIA6144 can be stored forfour years in alginate beads at 4 ◦C, maintaining its vitality and ability to establish a symbiosis thatstimulates early peanut growth. Understanding these physiological changes could be valuable forthe future improvement of long-lasting inoculants.
Fil: Cesari, Adriana Belen. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Fernandez, Marilina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Dardanelli, Marta Susana. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Materia
PGPR
Inoculant
Bacteria
Plant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/248229
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/248229
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early GrowthCesari, Adriana BelenFernandez, MarilinaPaulucci, Natalia SoledadDardanelli, Marta SusanaPGPRInoculantBacteriaPlanthttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Currently, bacterial inoculant technology focuses on improving long-term storage conditionsto ensure adequate rhizobia numbers and their effectiveness as plant growth promoters. Thisstudy aimed to investigate whether storage at 4 ◦C for four years of alginate beads immobilizingBradyrhizobium sp. SEMIA6144 maintains bacterial vitality, efficacy in growth promotion, and abilityto establish early interactions with Arachis hypogaea L. The recovery of viable SEMIA6144 cellsdecreased over time (10% at six months, 1% at one year, and 0.01% at four years), while cell vitalityremained high at 94.1%, 90.2%, and 93.4%, respectively. The unsaturated/saturated fatty acid ratiodeclined during storage, reducing membrane fluidity and metabolic activity. Mobility and rootadhesion of SEMIA6144 decreased after one and four years. However, growth promotion in peanutsinoculated with SEMIA6144 beads was observed through increased biomass, total chlorophyll, leafnumber, leaf area, and decreased chlorophyll fluorescence compared to non-inoculated plants. Althoughnodulation was low in plants inoculated with four-year-old beads, leghemoglobin levelswere maintained. These results demonstrate that Bradyrhizobium sp. SEMIA6144 can be stored forfour years in alginate beads at 4 ◦C, maintaining its vitality and ability to establish a symbiosis thatstimulates early peanut growth. Understanding these physiological changes could be valuable forthe future improvement of long-lasting inoculants.Fil: Cesari, Adriana Belen. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Fernandez, Marilina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Paulucci, Natalia Soledad. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Dardanelli, Marta Susana. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaMDPI2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/248229Cesari, Adriana Belen; Fernandez, Marilina; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth; MDPI; Plants; 13; 21; 10-2024; 1-202223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/13/21/2983info:eu-repo/semantics/altIdentifier/doi/10.3390/plants13212983info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:31Zoai:ri.conicet.gov.ar:11336/248229instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:32.215CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
title Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
spellingShingle Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
Cesari, Adriana Belen
PGPR
Inoculant
Bacteria
Plant
title_short Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
title_full Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
title_fullStr Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
title_full_unstemmed Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
title_sort Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth
dc.creator.none.fl_str_mv Cesari, Adriana Belen
Fernandez, Marilina
Paulucci, Natalia Soledad
Dardanelli, Marta Susana
author Cesari, Adriana Belen
author_facet Cesari, Adriana Belen
Fernandez, Marilina
Paulucci, Natalia Soledad
Dardanelli, Marta Susana
author_role author
author2 Fernandez, Marilina
Paulucci, Natalia Soledad
Dardanelli, Marta Susana
author2_role author
author
author
dc.subject.none.fl_str_mv PGPR
Inoculant
Bacteria
Plant
topic PGPR
Inoculant
Bacteria
Plant
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Currently, bacterial inoculant technology focuses on improving long-term storage conditionsto ensure adequate rhizobia numbers and their effectiveness as plant growth promoters. Thisstudy aimed to investigate whether storage at 4 ◦C for four years of alginate beads immobilizingBradyrhizobium sp. SEMIA6144 maintains bacterial vitality, efficacy in growth promotion, and abilityto establish early interactions with Arachis hypogaea L. The recovery of viable SEMIA6144 cellsdecreased over time (10% at six months, 1% at one year, and 0.01% at four years), while cell vitalityremained high at 94.1%, 90.2%, and 93.4%, respectively. The unsaturated/saturated fatty acid ratiodeclined during storage, reducing membrane fluidity and metabolic activity. Mobility and rootadhesion of SEMIA6144 decreased after one and four years. However, growth promotion in peanutsinoculated with SEMIA6144 beads was observed through increased biomass, total chlorophyll, leafnumber, leaf area, and decreased chlorophyll fluorescence compared to non-inoculated plants. Althoughnodulation was low in plants inoculated with four-year-old beads, leghemoglobin levelswere maintained. These results demonstrate that Bradyrhizobium sp. SEMIA6144 can be stored forfour years in alginate beads at 4 ◦C, maintaining its vitality and ability to establish a symbiosis thatstimulates early peanut growth. Understanding these physiological changes could be valuable forthe future improvement of long-lasting inoculants.
Fil: Cesari, Adriana Belen. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Fernandez, Marilina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Dardanelli, Marta Susana. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
description Currently, bacterial inoculant technology focuses on improving long-term storage conditionsto ensure adequate rhizobia numbers and their effectiveness as plant growth promoters. Thisstudy aimed to investigate whether storage at 4 ◦C for four years of alginate beads immobilizingBradyrhizobium sp. SEMIA6144 maintains bacterial vitality, efficacy in growth promotion, and abilityto establish early interactions with Arachis hypogaea L. The recovery of viable SEMIA6144 cellsdecreased over time (10% at six months, 1% at one year, and 0.01% at four years), while cell vitalityremained high at 94.1%, 90.2%, and 93.4%, respectively. The unsaturated/saturated fatty acid ratiodeclined during storage, reducing membrane fluidity and metabolic activity. Mobility and rootadhesion of SEMIA6144 decreased after one and four years. However, growth promotion in peanutsinoculated with SEMIA6144 beads was observed through increased biomass, total chlorophyll, leafnumber, leaf area, and decreased chlorophyll fluorescence compared to non-inoculated plants. Althoughnodulation was low in plants inoculated with four-year-old beads, leghemoglobin levelswere maintained. These results demonstrate that Bradyrhizobium sp. SEMIA6144 can be stored forfour years in alginate beads at 4 ◦C, maintaining its vitality and ability to establish a symbiosis thatstimulates early peanut growth. Understanding these physiological changes could be valuable forthe future improvement of long-lasting inoculants.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/248229
Cesari, Adriana Belen; Fernandez, Marilina; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth; MDPI; Plants; 13; 21; 10-2024; 1-20
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/248229
identifier_str_mv Cesari, Adriana Belen; Fernandez, Marilina; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early Growth; MDPI; Plants; 13; 21; 10-2024; 1-20
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/13/21/2983
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants13212983
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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