Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor

Autores
Garcia Gutierrez, María S.; Ortega Alvaro, Antonio; Busquets García, Arnau; Perez Ortiz, José M.; Caltana, Laura Romina; Ricatti, María Jimena; Brusco, Herminia Alicia; Maldonado, Rafael; Manzanares, Jorge
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, the role of CB2r on aversive memory consolidation was further evaluated. Mice lacking CB2r (CB2KO) and their corresponding littermates (WT) were exposed to the step-down inhibitory avoidance test (SDIA). MAP2, NF200 and synaptophysin (SYN)-immunoreactive fibers were studied in the hippocampus (HIP) of both genotypes. The number of synapses, postsynaptic density thickness and the relation between the synaptic length across the synaptic cleft and the distance between the synaptic ends were evaluated in the HIP (dentate gyrus (DG) and CA1 fields) by electron microscopy. Brainderived neurotrophic factor (BDNF), glucocorticoid receptor (NR3C1) gene expressions and mTOR/ p70S6K signaling cascade were evaluated in the HIP and prefrontal cortex (PFC). Finally, the effects of acute administration of CB2r-agonist JWH133 or CB2r-antagonist AM630 on memory consolidation were evaluated in WT mice by using the SDIA.
The lack of CB2r impaired aversive memory consolidation, reduced MAP2, NF200 and SYNimmunoreactive fibers and also reduced the number of synapses in DG of CB2KO mice. BDNF and NR3C1 gene expression were reduced in the HIP of CB2KO mice. An increase of p-p70S6K (T389 and S424) and p-AKT protein expression was observed in the HIP and PFC of CB2KO mice. Interestingly, administration of AM630 impaired aversive memory consolidation, whereas JWH133 enhanced it. Further functional and molecular assessments would have been helpful to further support our conclusions. These results revealed that CB2r are involved in memory consolidation, suggesting that this receptor could be a promising target for developing novel treatments for different cognitive impairmentrelated disorders.
Fil: Garcia Gutierrez, María S.. Consejo Superior de Investigaciones Cientificas; España. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Ortega Alvaro, Antonio. Universitat Pompeu Fabra; España. Complejo Hospitalario Universitario de Albacete; España
Fil: Busquets García, Arnau. Universitat Pompeu Fabra; España
Fil: Perez Ortiz, José M.. Complejo Hospitalario Universitario de Albacete; España
Fil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Ricatti, María Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Maldonado, Rafael. Universitat Pompeu Fabra; España
Fil: Manzanares, Jorge. Consejo Superior de Investigaciones Cientificas; España. Universidad Carlos III de Madrid. Instituto de Salud; España
Materia
Cb2r
Mice Laking Cb2r
Memory
Cannabinoid Cb2r-Agonist Jwh133
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8363
Acceder
id CONICETDig_7e57094b84f7b2c948c3f6e99fb09e26
oai_identifier_str oai:ri.conicet.gov.ar:11336/8363
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptorGarcia Gutierrez, María S.Ortega Alvaro, AntonioBusquets García, ArnauPerez Ortiz, José M.Caltana, Laura RominaRicatti, María JimenaBrusco, Herminia AliciaMaldonado, RafaelManzanares, JorgeCb2rMice Laking Cb2rMemoryCannabinoid Cb2r-Agonist Jwh133https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In this study, the role of CB2r on aversive memory consolidation was further evaluated. Mice lacking CB2r (CB2KO) and their corresponding littermates (WT) were exposed to the step-down inhibitory avoidance test (SDIA). MAP2, NF200 and synaptophysin (SYN)-immunoreactive fibers were studied in the hippocampus (HIP) of both genotypes. The number of synapses, postsynaptic density thickness and the relation between the synaptic length across the synaptic cleft and the distance between the synaptic ends were evaluated in the HIP (dentate gyrus (DG) and CA1 fields) by electron microscopy. Brainderived neurotrophic factor (BDNF), glucocorticoid receptor (NR3C1) gene expressions and mTOR/ p70S6K signaling cascade were evaluated in the HIP and prefrontal cortex (PFC). Finally, the effects of acute administration of CB2r-agonist JWH133 or CB2r-antagonist AM630 on memory consolidation were evaluated in WT mice by using the SDIA.<br />The lack of CB2r impaired aversive memory consolidation, reduced MAP2, NF200 and SYNimmunoreactive fibers and also reduced the number of synapses in DG of CB2KO mice. BDNF and NR3C1 gene expression were reduced in the HIP of CB2KO mice. An increase of p-p70S6K (T389 and S424) and p-AKT protein expression was observed in the HIP and PFC of CB2KO mice. Interestingly, administration of AM630 impaired aversive memory consolidation, whereas JWH133 enhanced it. Further functional and molecular assessments would have been helpful to further support our conclusions. These results revealed that CB2r are involved in memory consolidation, suggesting that this receptor could be a promising target for developing novel treatments for different cognitive impairmentrelated disorders.Fil: Garcia Gutierrez, María S.. Consejo Superior de Investigaciones Cientificas; España. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Ortega Alvaro, Antonio. Universitat Pompeu Fabra; España. Complejo Hospitalario Universitario de Albacete; EspañaFil: Busquets García, Arnau. Universitat Pompeu Fabra; EspañaFil: Perez Ortiz, José M.. Complejo Hospitalario Universitario de Albacete; EspañaFil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; ArgentinaFil: Ricatti, María Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; ArgentinaFil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; ArgentinaFil: Maldonado, Rafael. Universitat Pompeu Fabra; EspañaFil: Manzanares, Jorge. Consejo Superior de Investigaciones Cientificas; España. Universidad Carlos III de Madrid. Instituto de Salud; EspañaElsevier2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8363Garcia Gutierrez, María S.; Ortega Alvaro, Antonio; Busquets García, Arnau; Perez Ortiz, José M.; Caltana, Laura Romina; et al.; Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor; Elsevier; Neuropharmacology; 73; 5-2013; 388-3960028-3908enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0028390813002487info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuropharm.2013.05.034info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:07Zoai:ri.conicet.gov.ar:11336/8363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:07.841CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
title Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
spellingShingle Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
Garcia Gutierrez, María S.
Cb2r
Mice Laking Cb2r
Memory
Cannabinoid Cb2r-Agonist Jwh133
title_short Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
title_full Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
title_fullStr Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
title_full_unstemmed Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
title_sort Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor
dc.creator.none.fl_str_mv Garcia Gutierrez, María S.
Ortega Alvaro, Antonio
Busquets García, Arnau
Perez Ortiz, José M.
Caltana, Laura Romina
Ricatti, María Jimena
Brusco, Herminia Alicia
Maldonado, Rafael
Manzanares, Jorge
author Garcia Gutierrez, María S.
author_facet Garcia Gutierrez, María S.
Ortega Alvaro, Antonio
Busquets García, Arnau
Perez Ortiz, José M.
Caltana, Laura Romina
Ricatti, María Jimena
Brusco, Herminia Alicia
Maldonado, Rafael
Manzanares, Jorge
author_role author
author2 Ortega Alvaro, Antonio
Busquets García, Arnau
Perez Ortiz, José M.
Caltana, Laura Romina
Ricatti, María Jimena
Brusco, Herminia Alicia
Maldonado, Rafael
Manzanares, Jorge
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cb2r
Mice Laking Cb2r
Memory
Cannabinoid Cb2r-Agonist Jwh133
topic Cb2r
Mice Laking Cb2r
Memory
Cannabinoid Cb2r-Agonist Jwh133
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, the role of CB2r on aversive memory consolidation was further evaluated. Mice lacking CB2r (CB2KO) and their corresponding littermates (WT) were exposed to the step-down inhibitory avoidance test (SDIA). MAP2, NF200 and synaptophysin (SYN)-immunoreactive fibers were studied in the hippocampus (HIP) of both genotypes. The number of synapses, postsynaptic density thickness and the relation between the synaptic length across the synaptic cleft and the distance between the synaptic ends were evaluated in the HIP (dentate gyrus (DG) and CA1 fields) by electron microscopy. Brainderived neurotrophic factor (BDNF), glucocorticoid receptor (NR3C1) gene expressions and mTOR/ p70S6K signaling cascade were evaluated in the HIP and prefrontal cortex (PFC). Finally, the effects of acute administration of CB2r-agonist JWH133 or CB2r-antagonist AM630 on memory consolidation were evaluated in WT mice by using the SDIA.<br />The lack of CB2r impaired aversive memory consolidation, reduced MAP2, NF200 and SYNimmunoreactive fibers and also reduced the number of synapses in DG of CB2KO mice. BDNF and NR3C1 gene expression were reduced in the HIP of CB2KO mice. An increase of p-p70S6K (T389 and S424) and p-AKT protein expression was observed in the HIP and PFC of CB2KO mice. Interestingly, administration of AM630 impaired aversive memory consolidation, whereas JWH133 enhanced it. Further functional and molecular assessments would have been helpful to further support our conclusions. These results revealed that CB2r are involved in memory consolidation, suggesting that this receptor could be a promising target for developing novel treatments for different cognitive impairmentrelated disorders.
Fil: Garcia Gutierrez, María S.. Consejo Superior de Investigaciones Cientificas; España. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Ortega Alvaro, Antonio. Universitat Pompeu Fabra; España. Complejo Hospitalario Universitario de Albacete; España
Fil: Busquets García, Arnau. Universitat Pompeu Fabra; España
Fil: Perez Ortiz, José M.. Complejo Hospitalario Universitario de Albacete; España
Fil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Ricatti, María Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Maldonado, Rafael. Universitat Pompeu Fabra; España
Fil: Manzanares, Jorge. Consejo Superior de Investigaciones Cientificas; España. Universidad Carlos III de Madrid. Instituto de Salud; España
description In this study, the role of CB2r on aversive memory consolidation was further evaluated. Mice lacking CB2r (CB2KO) and their corresponding littermates (WT) were exposed to the step-down inhibitory avoidance test (SDIA). MAP2, NF200 and synaptophysin (SYN)-immunoreactive fibers were studied in the hippocampus (HIP) of both genotypes. The number of synapses, postsynaptic density thickness and the relation between the synaptic length across the synaptic cleft and the distance between the synaptic ends were evaluated in the HIP (dentate gyrus (DG) and CA1 fields) by electron microscopy. Brainderived neurotrophic factor (BDNF), glucocorticoid receptor (NR3C1) gene expressions and mTOR/ p70S6K signaling cascade were evaluated in the HIP and prefrontal cortex (PFC). Finally, the effects of acute administration of CB2r-agonist JWH133 or CB2r-antagonist AM630 on memory consolidation were evaluated in WT mice by using the SDIA.<br />The lack of CB2r impaired aversive memory consolidation, reduced MAP2, NF200 and SYNimmunoreactive fibers and also reduced the number of synapses in DG of CB2KO mice. BDNF and NR3C1 gene expression were reduced in the HIP of CB2KO mice. An increase of p-p70S6K (T389 and S424) and p-AKT protein expression was observed in the HIP and PFC of CB2KO mice. Interestingly, administration of AM630 impaired aversive memory consolidation, whereas JWH133 enhanced it. Further functional and molecular assessments would have been helpful to further support our conclusions. These results revealed that CB2r are involved in memory consolidation, suggesting that this receptor could be a promising target for developing novel treatments for different cognitive impairmentrelated disorders.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8363
Garcia Gutierrez, María S.; Ortega Alvaro, Antonio; Busquets García, Arnau; Perez Ortiz, José M.; Caltana, Laura Romina; et al.; Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor; Elsevier; Neuropharmacology; 73; 5-2013; 388-396
0028-3908
url http://hdl.handle.net/11336/8363
identifier_str_mv Garcia Gutierrez, María S.; Ortega Alvaro, Antonio; Busquets García, Arnau; Perez Ortiz, José M.; Caltana, Laura Romina; et al.; Synaptic plasticity alterations associated to memory impairment induced by deletion of CB2 cannabinoid receptor; Elsevier; Neuropharmacology; 73; 5-2013; 388-396
0028-3908
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0028390813002487
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuropharm.2013.05.034
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269620743962624
score 13.13397

Publicaciones similares