Evidence of cannabinoid modulation in nuclear signaling

Autores
Gaveglio, Virginia Lucía; Giusto, Norma Maria; Pasquaré, Susana Juana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The endocannabinoid system (ECS) is a signaling mechanism involved in many pathophysiological processes, especially in the nervous system. Former studies from our lab demonstrated the presence of the different components of the ECS in nuclei from rat cerebellum and cerebral cortex (CC). We detected diacylglycerol lipase and monoacylglycerol lipase activities which are involved in maintaining the levels of the endocannabinoid 2-arachidonoyl-glycerol. In addition, we demonstrated a nuclear CB1 protein expression by Western Blot and immunocytochemistry. CB1 is a GPCR receptor that triggers different signaling cascades, modulating intracellular Ca²+ levels, ERK1/2 phosphorylation, and other second messengers at the plasma membrane. Nevertheless, we also observed an increase in ERK phosphorylation in isolated nuclei from rat cerebral cortex (CCN) incubated with a CB1 synthetic agonist (WIN 55-212-2). Phosphorylated ERK could be involved in activating MSK1/2, a nuclear kinase that phosphorylates histone 3 (H3). The aim of this work was therefore to study how a cannabinoid agonist modulates ERK1/2 and H3 phosphorylation in CCN. To this end, CC from Wistar rats were dissected and homogenized, and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CCN were subsequently incubated at 37 °C with WIN and ERK1/2 and H3 signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 and H3 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.05). As expected, this was reversed by pre-incubating for 10 min with a MEK inhibitor (U0126, 10 µM), however, no changes were observed when a CB1 antagonist (SR141716, 1 µM) was used (p<0.05). Taken together, these results demonstrate that cannabinoids at nuclear level could modulate H3 phosphorylation by ERK1/2 signaling. This indicates a potential role of these lipids in chromatin regulation and gene expression in cerebral cortex
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica ; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología ; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
Materia
CANNABINOIDS
NUCLEI
CEREBRAL CORTEX
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/187829
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/187829
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network_name_str CONICET Digital (CONICET)
spelling Evidence of cannabinoid modulation in nuclear signalingGaveglio, Virginia LucíaGiusto, Norma MariaPasquaré, Susana JuanaCANNABINOIDSNUCLEICEREBRAL CORTEXhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The endocannabinoid system (ECS) is a signaling mechanism involved in many pathophysiological processes, especially in the nervous system. Former studies from our lab demonstrated the presence of the different components of the ECS in nuclei from rat cerebellum and cerebral cortex (CC). We detected diacylglycerol lipase and monoacylglycerol lipase activities which are involved in maintaining the levels of the endocannabinoid 2-arachidonoyl-glycerol. In addition, we demonstrated a nuclear CB1 protein expression by Western Blot and immunocytochemistry. CB1 is a GPCR receptor that triggers different signaling cascades, modulating intracellular Ca²+ levels, ERK1/2 phosphorylation, and other second messengers at the plasma membrane. Nevertheless, we also observed an increase in ERK phosphorylation in isolated nuclei from rat cerebral cortex (CCN) incubated with a CB1 synthetic agonist (WIN 55-212-2). Phosphorylated ERK could be involved in activating MSK1/2, a nuclear kinase that phosphorylates histone 3 (H3). The aim of this work was therefore to study how a cannabinoid agonist modulates ERK1/2 and H3 phosphorylation in CCN. To this end, CC from Wistar rats were dissected and homogenized, and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CCN were subsequently incubated at 37 °C with WIN and ERK1/2 and H3 signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 and H3 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.05). As expected, this was reversed by pre-incubating for 10 min with a MEK inhibitor (U0126, 10 µM), however, no changes were observed when a CB1 antagonist (SR141716, 1 µM) was used (p<0.05). Taken together, these results demonstrate that cannabinoids at nuclear level could modulate H3 phosphorylation by ERK1/2 signaling. This indicates a potential role of these lipids in chromatin regulation and gene expression in cerebral cortexFil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica ; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología ; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioThe Histochemical SocietyFundación Revista MedicinaKotsias, Basilio Aristidesde Vito, EduardoNarvaiz Kantor, IsabelSemeniuk, Guillermo Basilio2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/187829Evidence of cannabinoid modulation in nuclear signaling; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica ; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología ; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio ; Mar del Plata; Argentina; 2019; 141-1420025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:38Zoai:ri.conicet.gov.ar:11336/187829instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:39.208CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of cannabinoid modulation in nuclear signaling
title Evidence of cannabinoid modulation in nuclear signaling
spellingShingle Evidence of cannabinoid modulation in nuclear signaling
Gaveglio, Virginia Lucía
CANNABINOIDS
NUCLEI
CEREBRAL CORTEX
title_short Evidence of cannabinoid modulation in nuclear signaling
title_full Evidence of cannabinoid modulation in nuclear signaling
title_fullStr Evidence of cannabinoid modulation in nuclear signaling
title_full_unstemmed Evidence of cannabinoid modulation in nuclear signaling
title_sort Evidence of cannabinoid modulation in nuclear signaling
dc.creator.none.fl_str_mv Gaveglio, Virginia Lucía
Giusto, Norma Maria
Pasquaré, Susana Juana
author Gaveglio, Virginia Lucía
author_facet Gaveglio, Virginia Lucía
Giusto, Norma Maria
Pasquaré, Susana Juana
author_role author
author2 Giusto, Norma Maria
Pasquaré, Susana Juana
author2_role author
author
dc.contributor.none.fl_str_mv Kotsias, Basilio Aristides
de Vito, Eduardo
Narvaiz Kantor, Isabel
Semeniuk, Guillermo Basilio
dc.subject.none.fl_str_mv CANNABINOIDS
NUCLEI
CEREBRAL CORTEX
topic CANNABINOIDS
NUCLEI
CEREBRAL CORTEX
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The endocannabinoid system (ECS) is a signaling mechanism involved in many pathophysiological processes, especially in the nervous system. Former studies from our lab demonstrated the presence of the different components of the ECS in nuclei from rat cerebellum and cerebral cortex (CC). We detected diacylglycerol lipase and monoacylglycerol lipase activities which are involved in maintaining the levels of the endocannabinoid 2-arachidonoyl-glycerol. In addition, we demonstrated a nuclear CB1 protein expression by Western Blot and immunocytochemistry. CB1 is a GPCR receptor that triggers different signaling cascades, modulating intracellular Ca²+ levels, ERK1/2 phosphorylation, and other second messengers at the plasma membrane. Nevertheless, we also observed an increase in ERK phosphorylation in isolated nuclei from rat cerebral cortex (CCN) incubated with a CB1 synthetic agonist (WIN 55-212-2). Phosphorylated ERK could be involved in activating MSK1/2, a nuclear kinase that phosphorylates histone 3 (H3). The aim of this work was therefore to study how a cannabinoid agonist modulates ERK1/2 and H3 phosphorylation in CCN. To this end, CC from Wistar rats were dissected and homogenized, and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CCN were subsequently incubated at 37 °C with WIN and ERK1/2 and H3 signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 and H3 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.05). As expected, this was reversed by pre-incubating for 10 min with a MEK inhibitor (U0126, 10 µM), however, no changes were observed when a CB1 antagonist (SR141716, 1 µM) was used (p<0.05). Taken together, these results demonstrate that cannabinoids at nuclear level could modulate H3 phosphorylation by ERK1/2 signaling. This indicates a potential role of these lipids in chromatin regulation and gene expression in cerebral cortex
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica ; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología ; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
description The endocannabinoid system (ECS) is a signaling mechanism involved in many pathophysiological processes, especially in the nervous system. Former studies from our lab demonstrated the presence of the different components of the ECS in nuclei from rat cerebellum and cerebral cortex (CC). We detected diacylglycerol lipase and monoacylglycerol lipase activities which are involved in maintaining the levels of the endocannabinoid 2-arachidonoyl-glycerol. In addition, we demonstrated a nuclear CB1 protein expression by Western Blot and immunocytochemistry. CB1 is a GPCR receptor that triggers different signaling cascades, modulating intracellular Ca²+ levels, ERK1/2 phosphorylation, and other second messengers at the plasma membrane. Nevertheless, we also observed an increase in ERK phosphorylation in isolated nuclei from rat cerebral cortex (CCN) incubated with a CB1 synthetic agonist (WIN 55-212-2). Phosphorylated ERK could be involved in activating MSK1/2, a nuclear kinase that phosphorylates histone 3 (H3). The aim of this work was therefore to study how a cannabinoid agonist modulates ERK1/2 and H3 phosphorylation in CCN. To this end, CC from Wistar rats were dissected and homogenized, and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CCN were subsequently incubated at 37 °C with WIN and ERK1/2 and H3 signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 and H3 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.05). As expected, this was reversed by pre-incubating for 10 min with a MEK inhibitor (U0126, 10 µM), however, no changes were observed when a CB1 antagonist (SR141716, 1 µM) was used (p<0.05). Taken together, these results demonstrate that cannabinoids at nuclear level could modulate H3 phosphorylation by ERK1/2 signaling. This indicates a potential role of these lipids in chromatin regulation and gene expression in cerebral cortex
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/187829
Evidence of cannabinoid modulation in nuclear signaling; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica ; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología ; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio ; Mar del Plata; Argentina; 2019; 141-142
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/187829
identifier_str_mv Evidence of cannabinoid modulation in nuclear signaling; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica ; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental ; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología ; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio ; Mar del Plata; Argentina; 2019; 141-142
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anual
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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