Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells

Autores
Vermeulen, Elba Monica; Giordano, Mirta Nilda; Trevani, Analía Silvina; Sedlik, Christine; Gamberale, Romina; Fernández Calotti, Paula; Salamone, Gabriela Veronica; Raiden, Silvina Claudia; Sanjurjo, Julieta; Geffner, Jorge Raúl
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is widely appreciated that inflammatory responses in peripheral tissues are usually associated to the development of acidic microenvironments. Despite this, there are few studies aimed to analyze the effect of extracellular pH on immune cell functions. We analyzed the impact of acidosis on the behavior of dendritic cells (DCs) derived from murine bone marrow. We found that extracellular acidosis (pH 6.5) markedly stimulated the uptake of FITC-OVA, FITC-dextran, and HRP by DCs. In fact, to reach similar levels of endocytosis, DCs cultured at pH 7.3 required concentrations of Ag in the extracellular medium almost 10-fold higher compared with DCs cultured at pH 6.5. Not only the endocytic capacity of DCs was up-regulated by extracellular acidosis, but also the expression of CD11c, MHC class II, CD40, and CD86 as well as the acquisition of extracellular Ags by DCs for MHC class I-restricted presentation. Importantly, DCs pulsed with Ag under acidosis showed an improved efficacy to induce both specific CD8+ CTLs and specific Ab responses in vivo. Our results suggest that extracellular acidosis improves the Ag-presenting capacity of DCs.
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Giordano, Mirta Nilda. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Sedlik, Christine. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Gamberale, Romina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Calotti, Paula. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Salamone, Gabriela Veronica. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Raiden, Silvina Claudia. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanjurjo, Julieta. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Materia
dendritic cell
acidosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95967
Acceder
id CONICETDig_cfef7ba504ad8e475f5adea4ca7dfb41
oai_identifier_str oai:ri.conicet.gov.ar:11336/95967
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic CellsVermeulen, Elba MonicaGiordano, Mirta NildaTrevani, Analía SilvinaSedlik, ChristineGamberale, RominaFernández Calotti, PaulaSalamone, Gabriela VeronicaRaiden, Silvina ClaudiaSanjurjo, JulietaGeffner, Jorge Raúldendritic cellacidosishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It is widely appreciated that inflammatory responses in peripheral tissues are usually associated to the development of acidic microenvironments. Despite this, there are few studies aimed to analyze the effect of extracellular pH on immune cell functions. We analyzed the impact of acidosis on the behavior of dendritic cells (DCs) derived from murine bone marrow. We found that extracellular acidosis (pH 6.5) markedly stimulated the uptake of FITC-OVA, FITC-dextran, and HRP by DCs. In fact, to reach similar levels of endocytosis, DCs cultured at pH 7.3 required concentrations of Ag in the extracellular medium almost 10-fold higher compared with DCs cultured at pH 6.5. Not only the endocytic capacity of DCs was up-regulated by extracellular acidosis, but also the expression of CD11c, MHC class II, CD40, and CD86 as well as the acquisition of extracellular Ags by DCs for MHC class I-restricted presentation. Importantly, DCs pulsed with Ag under acidosis showed an improved efficacy to induce both specific CD8+ CTLs and specific Ab responses in vivo. Our results suggest that extracellular acidosis improves the Ag-presenting capacity of DCs.Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Giordano, Mirta Nilda. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Sedlik, Christine. Institut National de la Santé et de la Recherche Médicale; FranciaFil: Gamberale, Romina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández Calotti, Paula. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Salamone, Gabriela Veronica. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Raiden, Silvina Claudia. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanjurjo, Julieta. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaAmerican Association of Immunologists2004-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95967Vermeulen, Elba Monica; Giordano, Mirta Nilda; Trevani, Analía Silvina; Sedlik, Christine; Gamberale, Romina; et al.; Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells; American Association of Immunologists; Journal of Immunology; 172; 5; 3-2004; 3196-32040022-1767CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jimmunol.org/content/172/5/3196info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.172.5.3196info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:46Zoai:ri.conicet.gov.ar:11336/95967instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:46.655CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
title Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
spellingShingle Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
Vermeulen, Elba Monica
dendritic cell
acidosis
title_short Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
title_full Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
title_fullStr Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
title_full_unstemmed Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
title_sort Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells
dc.creator.none.fl_str_mv Vermeulen, Elba Monica
Giordano, Mirta Nilda
Trevani, Analía Silvina
Sedlik, Christine
Gamberale, Romina
Fernández Calotti, Paula
Salamone, Gabriela Veronica
Raiden, Silvina Claudia
Sanjurjo, Julieta
Geffner, Jorge Raúl
author Vermeulen, Elba Monica
author_facet Vermeulen, Elba Monica
Giordano, Mirta Nilda
Trevani, Analía Silvina
Sedlik, Christine
Gamberale, Romina
Fernández Calotti, Paula
Salamone, Gabriela Veronica
Raiden, Silvina Claudia
Sanjurjo, Julieta
Geffner, Jorge Raúl
author_role author
author2 Giordano, Mirta Nilda
Trevani, Analía Silvina
Sedlik, Christine
Gamberale, Romina
Fernández Calotti, Paula
Salamone, Gabriela Veronica
Raiden, Silvina Claudia
Sanjurjo, Julieta
Geffner, Jorge Raúl
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv dendritic cell
acidosis
topic dendritic cell
acidosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It is widely appreciated that inflammatory responses in peripheral tissues are usually associated to the development of acidic microenvironments. Despite this, there are few studies aimed to analyze the effect of extracellular pH on immune cell functions. We analyzed the impact of acidosis on the behavior of dendritic cells (DCs) derived from murine bone marrow. We found that extracellular acidosis (pH 6.5) markedly stimulated the uptake of FITC-OVA, FITC-dextran, and HRP by DCs. In fact, to reach similar levels of endocytosis, DCs cultured at pH 7.3 required concentrations of Ag in the extracellular medium almost 10-fold higher compared with DCs cultured at pH 6.5. Not only the endocytic capacity of DCs was up-regulated by extracellular acidosis, but also the expression of CD11c, MHC class II, CD40, and CD86 as well as the acquisition of extracellular Ags by DCs for MHC class I-restricted presentation. Importantly, DCs pulsed with Ag under acidosis showed an improved efficacy to induce both specific CD8+ CTLs and specific Ab responses in vivo. Our results suggest that extracellular acidosis improves the Ag-presenting capacity of DCs.
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina
Fil: Giordano, Mirta Nilda. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Sedlik, Christine. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Gamberale, Romina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Calotti, Paula. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Salamone, Gabriela Veronica. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Raiden, Silvina Claudia. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanjurjo, Julieta. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
description It is widely appreciated that inflammatory responses in peripheral tissues are usually associated to the development of acidic microenvironments. Despite this, there are few studies aimed to analyze the effect of extracellular pH on immune cell functions. We analyzed the impact of acidosis on the behavior of dendritic cells (DCs) derived from murine bone marrow. We found that extracellular acidosis (pH 6.5) markedly stimulated the uptake of FITC-OVA, FITC-dextran, and HRP by DCs. In fact, to reach similar levels of endocytosis, DCs cultured at pH 7.3 required concentrations of Ag in the extracellular medium almost 10-fold higher compared with DCs cultured at pH 6.5. Not only the endocytic capacity of DCs was up-regulated by extracellular acidosis, but also the expression of CD11c, MHC class II, CD40, and CD86 as well as the acquisition of extracellular Ags by DCs for MHC class I-restricted presentation. Importantly, DCs pulsed with Ag under acidosis showed an improved efficacy to induce both specific CD8+ CTLs and specific Ab responses in vivo. Our results suggest that extracellular acidosis improves the Ag-presenting capacity of DCs.
publishDate 2004
dc.date.none.fl_str_mv 2004-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95967
Vermeulen, Elba Monica; Giordano, Mirta Nilda; Trevani, Analía Silvina; Sedlik, Christine; Gamberale, Romina; et al.; Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells; American Association of Immunologists; Journal of Immunology; 172; 5; 3-2004; 3196-3204
0022-1767
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95967
identifier_str_mv Vermeulen, Elba Monica; Giordano, Mirta Nilda; Trevani, Analía Silvina; Sedlik, Christine; Gamberale, Romina; et al.; Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells; American Association of Immunologists; Journal of Immunology; 172; 5; 3-2004; 3196-3204
0022-1767
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jimmunol.org/content/172/5/3196
info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.172.5.3196
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269717263286272
score 13.13397

Publicaciones similares