Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells

Autores
del Balzo, Diego Damián; Capmany, Anahi; Cebrián, José Ignacio; Damiani, María ElenaTeresa
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Chlamydia trachomatis (CT) is the most frequent bacterial cause of sexually transmitted infections worldwide. This highly adapted intracellular bacterium has evolved multiple strategies to hide inside cells. However, little is known about the molecular mechanisms underlying CT evasion of the immune response. Dendritic cells (DCs) are the most efficient antigen-presenting cells of the immune system and, an essential link between innate and adaptive immunity. Therefore, DCs could play a key role in CT´s clearance. In this study, we analyzed in CT-infected DCs the process of cross-presentation, in which exogenous antigens are associated with MHC-I molecules to activate CD8+ T lymphocytes. By confocal microscopy and flow cytometry-based approaches, we observed, after chlamydial infection, a decrease in MHC-I molecules exposed at the plasma membrane while they are redistributed intracellularly. However, the total amount of MHC-I molecules did not change after infection, as assessed by western blot analysis. Finally, we found that CT-infected DCs were less efficient than non-infected ones to cross-present the model antigen Ovalbumin, as measured colorimetrically by the activation of the antigen-specific CD8+ T cell hybrid called B3Z. Altogether, these findings indicate that CT infection impairs antigen-cross presentation in DCs through the disturbance of MHC-I transport.
Fil: del Balzo, Diego Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Damiani, María ElenaTeresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XIV Conferencia de la Asociación Panamericana de Bioquímica y Biología Molecular
Salta
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Asociación Panamericana de Bioquímica y Biología Molecular
Materia
CHLAMYDIA TRACHOMATIS
CROSS-PRESENTATION
CMH-I
DENDRITIC CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235128
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/235128
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cellsdel Balzo, Diego DamiánCapmany, AnahiCebrián, José IgnacioDamiani, María ElenaTeresaCHLAMYDIA TRACHOMATISCROSS-PRESENTATIONCMH-IDENDRITIC CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chlamydia trachomatis (CT) is the most frequent bacterial cause of sexually transmitted infections worldwide. This highly adapted intracellular bacterium has evolved multiple strategies to hide inside cells. However, little is known about the molecular mechanisms underlying CT evasion of the immune response. Dendritic cells (DCs) are the most efficient antigen-presenting cells of the immune system and, an essential link between innate and adaptive immunity. Therefore, DCs could play a key role in CT´s clearance. In this study, we analyzed in CT-infected DCs the process of cross-presentation, in which exogenous antigens are associated with MHC-I molecules to activate CD8+ T lymphocytes. By confocal microscopy and flow cytometry-based approaches, we observed, after chlamydial infection, a decrease in MHC-I molecules exposed at the plasma membrane while they are redistributed intracellularly. However, the total amount of MHC-I molecules did not change after infection, as assessed by western blot analysis. Finally, we found that CT-infected DCs were less efficient than non-infected ones to cross-present the model antigen Ovalbumin, as measured colorimetrically by the activation of the antigen-specific CD8+ T cell hybrid called B3Z. Altogether, these findings indicate that CT infection impairs antigen-cross presentation in DCs through the disturbance of MHC-I transport.Fil: del Balzo, Diego Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Damiani, María ElenaTeresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XIV Conferencia de la Asociación Panamericana de Bioquímica y Biología MolecularSaltaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularAsociación Panamericana de Bioquímica y Biología MolecularTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235128Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells; LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XIV Conferencia de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 78-780327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:42Zoai:ri.conicet.gov.ar:11336/235128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:43.167CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
title Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
spellingShingle Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
del Balzo, Diego Damián
CHLAMYDIA TRACHOMATIS
CROSS-PRESENTATION
CMH-I
DENDRITIC CELLS
title_short Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
title_full Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
title_fullStr Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
title_full_unstemmed Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
title_sort Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells
dc.creator.none.fl_str_mv del Balzo, Diego Damián
Capmany, Anahi
Cebrián, José Ignacio
Damiani, María ElenaTeresa
author del Balzo, Diego Damián
author_facet del Balzo, Diego Damián
Capmany, Anahi
Cebrián, José Ignacio
Damiani, María ElenaTeresa
author_role author
author2 Capmany, Anahi
Cebrián, José Ignacio
Damiani, María ElenaTeresa
author2_role author
author
author
dc.subject.none.fl_str_mv CHLAMYDIA TRACHOMATIS
CROSS-PRESENTATION
CMH-I
DENDRITIC CELLS
topic CHLAMYDIA TRACHOMATIS
CROSS-PRESENTATION
CMH-I
DENDRITIC CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chlamydia trachomatis (CT) is the most frequent bacterial cause of sexually transmitted infections worldwide. This highly adapted intracellular bacterium has evolved multiple strategies to hide inside cells. However, little is known about the molecular mechanisms underlying CT evasion of the immune response. Dendritic cells (DCs) are the most efficient antigen-presenting cells of the immune system and, an essential link between innate and adaptive immunity. Therefore, DCs could play a key role in CT´s clearance. In this study, we analyzed in CT-infected DCs the process of cross-presentation, in which exogenous antigens are associated with MHC-I molecules to activate CD8+ T lymphocytes. By confocal microscopy and flow cytometry-based approaches, we observed, after chlamydial infection, a decrease in MHC-I molecules exposed at the plasma membrane while they are redistributed intracellularly. However, the total amount of MHC-I molecules did not change after infection, as assessed by western blot analysis. Finally, we found that CT-infected DCs were less efficient than non-infected ones to cross-present the model antigen Ovalbumin, as measured colorimetrically by the activation of the antigen-specific CD8+ T cell hybrid called B3Z. Altogether, these findings indicate that CT infection impairs antigen-cross presentation in DCs through the disturbance of MHC-I transport.
Fil: del Balzo, Diego Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Damiani, María ElenaTeresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XIV Conferencia de la Asociación Panamericana de Bioquímica y Biología Molecular
Salta
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Asociación Panamericana de Bioquímica y Biología Molecular
description Chlamydia trachomatis (CT) is the most frequent bacterial cause of sexually transmitted infections worldwide. This highly adapted intracellular bacterium has evolved multiple strategies to hide inside cells. However, little is known about the molecular mechanisms underlying CT evasion of the immune response. Dendritic cells (DCs) are the most efficient antigen-presenting cells of the immune system and, an essential link between innate and adaptive immunity. Therefore, DCs could play a key role in CT´s clearance. In this study, we analyzed in CT-infected DCs the process of cross-presentation, in which exogenous antigens are associated with MHC-I molecules to activate CD8+ T lymphocytes. By confocal microscopy and flow cytometry-based approaches, we observed, after chlamydial infection, a decrease in MHC-I molecules exposed at the plasma membrane while they are redistributed intracellularly. However, the total amount of MHC-I molecules did not change after infection, as assessed by western blot analysis. Finally, we found that CT-infected DCs were less efficient than non-infected ones to cross-present the model antigen Ovalbumin, as measured colorimetrically by the activation of the antigen-specific CD8+ T cell hybrid called B3Z. Altogether, these findings indicate that CT infection impairs antigen-cross presentation in DCs through the disturbance of MHC-I transport.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235128
Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells; LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XIV Conferencia de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 78-78
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235128
identifier_str_mv Chlamydia trachomatis causes the intracellular redistribution of MHC-I and impairs antigen presentation in dendritic cells; LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XIV Conferencia de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 78-78
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019.pdf
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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