Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off

Autores
Baravalle, Celina; Dallard, Bibiana Elisabet; Ortega, Hugo Hector; Neder, V.E.; Canavesio, V.R.; Calvinho, Luis Fernando
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biological response modifiers (BRM) are agents that modify the host's response to pathogens with resultant beneficial prophylactic or therapeutic effects. The objectives of this study were to describe the immunomodulatory effects of Panax ginseng (GS) on bovine mammary glands at the end of lactation. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of BRM (3 mg/mL), six quarters were treated with placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milk samples were collected at different time points for detection of specific cytokines mRNA by RT-PCR and Western blotting assay. A significant increase of IL-1α, IL-1β and TNF-α mRNA expression was observed in BRM-treated compared with placebo-treated quarters at 48 h post-treatment (pt) (P<0.05). A 17 kDa TNF-α band expressed a sharp elevation at 24 h and reduction in its level at 48 h pt in BRM-treated quarters. Differences in this cytokine level between 24 and 48 h pt times were significant (P<0.05). GS extract inoculation at drying off was associated with somatic cell counts increase, cytokines mRNA transcription and the presence of TNF-α in milk and can therefore exert immunomodulating effects in bovine mammary gland at drying off.
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Neder, V.E.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Canavesio, V.R.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina. Universidad Nacional del Litoral; Argentina
Materia
BOVINE MAMMARY GLAND
CYTOKINES
INVOLUTION
PANAX GINSENG
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97635
Acceder
id CONICETDig_cfa5cc1f88808814b4f6957db3b19327
oai_identifier_str oai:ri.conicet.gov.ar:11336/97635
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying offBaravalle, CelinaDallard, Bibiana ElisabetOrtega, Hugo HectorNeder, V.E.Canavesio, V.R.Calvinho, Luis FernandoBOVINE MAMMARY GLANDCYTOKINESINVOLUTIONPANAX GINSENGhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Biological response modifiers (BRM) are agents that modify the host's response to pathogens with resultant beneficial prophylactic or therapeutic effects. The objectives of this study were to describe the immunomodulatory effects of Panax ginseng (GS) on bovine mammary glands at the end of lactation. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of BRM (3 mg/mL), six quarters were treated with placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milk samples were collected at different time points for detection of specific cytokines mRNA by RT-PCR and Western blotting assay. A significant increase of IL-1α, IL-1β and TNF-α mRNA expression was observed in BRM-treated compared with placebo-treated quarters at 48 h post-treatment (pt) (P<0.05). A 17 kDa TNF-α band expressed a sharp elevation at 24 h and reduction in its level at 48 h pt in BRM-treated quarters. Differences in this cytokine level between 24 and 48 h pt times were significant (P<0.05). GS extract inoculation at drying off was associated with somatic cell counts increase, cytokines mRNA transcription and the presence of TNF-α in milk and can therefore exert immunomodulating effects in bovine mammary gland at drying off.Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Neder, V.E.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaFil: Canavesio, V.R.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina. Universidad Nacional del Litoral; ArgentinaElsevier Science2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97635Baravalle, Celina; Dallard, Bibiana Elisabet; Ortega, Hugo Hector; Neder, V.E.; Canavesio, V.R.; et al.; Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 138; 3; 12-2010; 224-2300165-2427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://sciencedirect.com/science/journal/01652427info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2010.07.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:18Zoai:ri.conicet.gov.ar:11336/97635instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:18.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
title Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
spellingShingle Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
Baravalle, Celina
BOVINE MAMMARY GLAND
CYTOKINES
INVOLUTION
PANAX GINSENG
title_short Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
title_full Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
title_fullStr Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
title_full_unstemmed Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
title_sort Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off
dc.creator.none.fl_str_mv Baravalle, Celina
Dallard, Bibiana Elisabet
Ortega, Hugo Hector
Neder, V.E.
Canavesio, V.R.
Calvinho, Luis Fernando
author Baravalle, Celina
author_facet Baravalle, Celina
Dallard, Bibiana Elisabet
Ortega, Hugo Hector
Neder, V.E.
Canavesio, V.R.
Calvinho, Luis Fernando
author_role author
author2 Dallard, Bibiana Elisabet
Ortega, Hugo Hector
Neder, V.E.
Canavesio, V.R.
Calvinho, Luis Fernando
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE MAMMARY GLAND
CYTOKINES
INVOLUTION
PANAX GINSENG
topic BOVINE MAMMARY GLAND
CYTOKINES
INVOLUTION
PANAX GINSENG
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Biological response modifiers (BRM) are agents that modify the host's response to pathogens with resultant beneficial prophylactic or therapeutic effects. The objectives of this study were to describe the immunomodulatory effects of Panax ginseng (GS) on bovine mammary glands at the end of lactation. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of BRM (3 mg/mL), six quarters were treated with placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milk samples were collected at different time points for detection of specific cytokines mRNA by RT-PCR and Western blotting assay. A significant increase of IL-1α, IL-1β and TNF-α mRNA expression was observed in BRM-treated compared with placebo-treated quarters at 48 h post-treatment (pt) (P<0.05). A 17 kDa TNF-α band expressed a sharp elevation at 24 h and reduction in its level at 48 h pt in BRM-treated quarters. Differences in this cytokine level between 24 and 48 h pt times were significant (P<0.05). GS extract inoculation at drying off was associated with somatic cell counts increase, cytokines mRNA transcription and the presence of TNF-α in milk and can therefore exert immunomodulating effects in bovine mammary gland at drying off.
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Neder, V.E.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Canavesio, V.R.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina. Universidad Nacional del Litoral; Argentina
description Biological response modifiers (BRM) are agents that modify the host's response to pathogens with resultant beneficial prophylactic or therapeutic effects. The objectives of this study were to describe the immunomodulatory effects of Panax ginseng (GS) on bovine mammary glands at the end of lactation. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of BRM (3 mg/mL), six quarters were treated with placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milk samples were collected at different time points for detection of specific cytokines mRNA by RT-PCR and Western blotting assay. A significant increase of IL-1α, IL-1β and TNF-α mRNA expression was observed in BRM-treated compared with placebo-treated quarters at 48 h post-treatment (pt) (P<0.05). A 17 kDa TNF-α band expressed a sharp elevation at 24 h and reduction in its level at 48 h pt in BRM-treated quarters. Differences in this cytokine level between 24 and 48 h pt times were significant (P<0.05). GS extract inoculation at drying off was associated with somatic cell counts increase, cytokines mRNA transcription and the presence of TNF-α in milk and can therefore exert immunomodulating effects in bovine mammary gland at drying off.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97635
Baravalle, Celina; Dallard, Bibiana Elisabet; Ortega, Hugo Hector; Neder, V.E.; Canavesio, V.R.; et al.; Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 138; 3; 12-2010; 224-230
0165-2427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97635
identifier_str_mv Baravalle, Celina; Dallard, Bibiana Elisabet; Ortega, Hugo Hector; Neder, V.E.; Canavesio, V.R.; et al.; Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 138; 3; 12-2010; 224-230
0165-2427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://sciencedirect.com/science/journal/01652427
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2010.07.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269952197787648
score 13.13397

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