Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off

Autores
Baravalle, Celina; Dallard, Bibiana Elisabet; Cadoche, M. C.; Pereyra, Elizabet Amanda Lorena; Neder, V. E.; Ortega, H. H.; Calvinho, L. F.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The lack of efficacy of conventional strategies for the maintenance of healthy udders in domestic cattle has prompted studies on the use of immunomodulators or biological response modifiers (BRM) for this purpose. These compounds are agents that modify the host's response to pathogens leading to beneficial effects on disease outcome. The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng (GS) extract on the amount of pro-inflammatory cytokines and the number of monocytes/macrophages present in bovine mammary tissues at drying off. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of GS (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. The analyses of tumor necrosis factor-alpha (TNF-α) by immunohistochemistry revealed that the production of this proinflammatory cytokine significantly increased (P < 0.05) in the inoculated mammary glands of cows following BRM inoculation, whereas the interleukin-1 alpha (IL-1α) and IL-6 staining area was not affected by BRM treatment. The number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) in BRM-treated quarters than in placebo and uninoculated control quarters. These results indicated an immunomodulator potential of the BRM used. The beneficial effect of the extract could be used as alternative therapy in the control of mastitis at drying off, either alone or in conjunction with dry cow antibiotic therapy.
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Cadoche, M. C.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina
Fil: Neder, V. E.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ortega, H. H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Calvinho, L. F.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Materia
Bovine Mammary Gland
Panax Ginseng
Immunohistochemistry
Cytokines
Cd14
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14569
Acceder
id CONICETDig_13c9506a890ecfb376d4660de1fc9c01
oai_identifier_str oai:ri.conicet.gov.ar:11336/14569
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying offBaravalle, CelinaDallard, Bibiana ElisabetCadoche, M. C.Pereyra, Elizabet Amanda LorenaNeder, V. E.Ortega, H. H.Calvinho, L. F.Bovine Mammary GlandPanax GinsengImmunohistochemistryCytokinesCd14https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The lack of efficacy of conventional strategies for the maintenance of healthy udders in domestic cattle has prompted studies on the use of immunomodulators or biological response modifiers (BRM) for this purpose. These compounds are agents that modify the host's response to pathogens leading to beneficial effects on disease outcome. The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng (GS) extract on the amount of pro-inflammatory cytokines and the number of monocytes/macrophages present in bovine mammary tissues at drying off. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of GS (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. The analyses of tumor necrosis factor-alpha (TNF-α) by immunohistochemistry revealed that the production of this proinflammatory cytokine significantly increased (P < 0.05) in the inoculated mammary glands of cows following BRM inoculation, whereas the interleukin-1 alpha (IL-1α) and IL-6 staining area was not affected by BRM treatment. The number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) in BRM-treated quarters than in placebo and uninoculated control quarters. These results indicated an immunomodulator potential of the BRM used. The beneficial effect of the extract could be used as alternative therapy in the control of mastitis at drying off, either alone or in conjunction with dry cow antibiotic therapy.Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; ArgentinaFil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; ArgentinaFil: Cadoche, M. C.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; ArgentinaFil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; ArgentinaFil: Neder, V. E.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Ortega, H. H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; ArgentinaFil: Calvinho, L. F.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaElsevier Science2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14569Baravalle, Celina; Dallard, Bibiana Elisabet; Cadoche, M. C.; Pereyra, Elizabet Amanda Lorena; Neder, V. E.; et al.; Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 144; 1-2; 10-2011; 52-600165-2427enginfo:eu-repo/semantics/altIdentifier/doi//10.1016/j.vetimm.2011.07.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0165242711002522info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:16Zoai:ri.conicet.gov.ar:11336/14569instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:17.303CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
title Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
spellingShingle Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
Baravalle, Celina
Bovine Mammary Gland
Panax Ginseng
Immunohistochemistry
Cytokines
Cd14
title_short Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
title_full Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
title_fullStr Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
title_full_unstemmed Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
title_sort Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off
dc.creator.none.fl_str_mv Baravalle, Celina
Dallard, Bibiana Elisabet
Cadoche, M. C.
Pereyra, Elizabet Amanda Lorena
Neder, V. E.
Ortega, H. H.
Calvinho, L. F.
author Baravalle, Celina
author_facet Baravalle, Celina
Dallard, Bibiana Elisabet
Cadoche, M. C.
Pereyra, Elizabet Amanda Lorena
Neder, V. E.
Ortega, H. H.
Calvinho, L. F.
author_role author
author2 Dallard, Bibiana Elisabet
Cadoche, M. C.
Pereyra, Elizabet Amanda Lorena
Neder, V. E.
Ortega, H. H.
Calvinho, L. F.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bovine Mammary Gland
Panax Ginseng
Immunohistochemistry
Cytokines
Cd14
topic Bovine Mammary Gland
Panax Ginseng
Immunohistochemistry
Cytokines
Cd14
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The lack of efficacy of conventional strategies for the maintenance of healthy udders in domestic cattle has prompted studies on the use of immunomodulators or biological response modifiers (BRM) for this purpose. These compounds are agents that modify the host's response to pathogens leading to beneficial effects on disease outcome. The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng (GS) extract on the amount of pro-inflammatory cytokines and the number of monocytes/macrophages present in bovine mammary tissues at drying off. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of GS (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. The analyses of tumor necrosis factor-alpha (TNF-α) by immunohistochemistry revealed that the production of this proinflammatory cytokine significantly increased (P < 0.05) in the inoculated mammary glands of cows following BRM inoculation, whereas the interleukin-1 alpha (IL-1α) and IL-6 staining area was not affected by BRM treatment. The number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) in BRM-treated quarters than in placebo and uninoculated control quarters. These results indicated an immunomodulator potential of the BRM used. The beneficial effect of the extract could be used as alternative therapy in the control of mastitis at drying off, either alone or in conjunction with dry cow antibiotic therapy.
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Cadoche, M. C.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina
Fil: Neder, V. E.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ortega, H. H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina
Fil: Calvinho, L. F.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias ; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
description The lack of efficacy of conventional strategies for the maintenance of healthy udders in domestic cattle has prompted studies on the use of immunomodulators or biological response modifiers (BRM) for this purpose. These compounds are agents that modify the host's response to pathogens leading to beneficial effects on disease outcome. The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng (GS) extract on the amount of pro-inflammatory cytokines and the number of monocytes/macrophages present in bovine mammary tissues at drying off. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of GS (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. The analyses of tumor necrosis factor-alpha (TNF-α) by immunohistochemistry revealed that the production of this proinflammatory cytokine significantly increased (P < 0.05) in the inoculated mammary glands of cows following BRM inoculation, whereas the interleukin-1 alpha (IL-1α) and IL-6 staining area was not affected by BRM treatment. The number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) in BRM-treated quarters than in placebo and uninoculated control quarters. These results indicated an immunomodulator potential of the BRM used. The beneficial effect of the extract could be used as alternative therapy in the control of mastitis at drying off, either alone or in conjunction with dry cow antibiotic therapy.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14569
Baravalle, Celina; Dallard, Bibiana Elisabet; Cadoche, M. C.; Pereyra, Elizabet Amanda Lorena; Neder, V. E.; et al.; Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 144; 1-2; 10-2011; 52-60
0165-2427
url http://hdl.handle.net/11336/14569
identifier_str_mv Baravalle, Celina; Dallard, Bibiana Elisabet; Cadoche, M. C.; Pereyra, Elizabet Amanda Lorena; Neder, V. E.; et al.; Proinflammatory cytokines and CD14 expression in mammary tissue of cows following intramammary inoculation of Panax ginseng at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 144; 1-2; 10-2011; 52-60
0165-2427
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi//10.1016/j.vetimm.2011.07.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0165242711002522
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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