Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities

Autores
Tula, Suresh; Shahinnia, Fahimeh; Melzer, Michael; Rutten, Twan; Gómez, Rodrigo; Lodeyro, Anabella Fernanda; von Wirén, Nicolaus; Carrillo, Nestor Jose; Hajirezaei, Mohammad R.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ability of plants to maintain photosynthesis in a dynamically changing environment is of central importance for their growth. As the photosynthetic machinery is a sensitive and early target of adverse environmental conditions as those typically found in the field, photosynthetic efficiency is not always optimal. Cyanobacteria, algae, mosses, liverworts and gymnosperms produce flavodiiron proteins (Flvs), a class of electron sinks not represented in angiosperms; these proteins act to mitigate the photoinhibition of photosystem I under high or fluctuating light. Here, genes specifying two cyanobacterial Flvs have been expressed in the chloroplasts of Arabidopsis thaliana in an attempt to improve plant growth. Co-expression of Flv1 and Flv3 enhanced the efficiency of light utilization, boosting the plant’s capacity to accumulate biomass as the growth light intensity was raised. The Flv1/Flv3 transgenics displayed an increased production of ATP, an acceleration of carbohydrate metabolism and a more pronounced partitioning of sucrose into starch. The results suggest that Flvs are able to establish an efficient electron sink downstream of PSI, thereby ensuring efficient photosynthetic electron transport at moderate to high light intensities. The expression of Flvs thus acts to both protect photosynthesis and to control the ATP/NADPH ratio; together, their presence is beneficial for the plant’s growth potential.
Fil: Tula, Suresh. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Shahinnia, Fahimeh. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Melzer, Michael. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Rutten, Twan. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Gómez, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Lodeyro, Anabella Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: von Wirén, Nicolaus. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hajirezaei, Mohammad R.. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Materia
A. THALIANA
BIOMASS
CYANOBACTERIA
ELECTRON SINK
FLAVODIIRON PROTEINS
PHOTOSYNTHESIS
PRIMARY METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184254
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184254
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light IntensitiesTula, SureshShahinnia, FahimehMelzer, MichaelRutten, TwanGómez, RodrigoLodeyro, Anabella Fernandavon Wirén, NicolausCarrillo, Nestor JoseHajirezaei, Mohammad R.A. THALIANABIOMASSCYANOBACTERIAELECTRON SINKFLAVODIIRON PROTEINSPHOTOSYNTHESISPRIMARY METABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ability of plants to maintain photosynthesis in a dynamically changing environment is of central importance for their growth. As the photosynthetic machinery is a sensitive and early target of adverse environmental conditions as those typically found in the field, photosynthetic efficiency is not always optimal. Cyanobacteria, algae, mosses, liverworts and gymnosperms produce flavodiiron proteins (Flvs), a class of electron sinks not represented in angiosperms; these proteins act to mitigate the photoinhibition of photosystem I under high or fluctuating light. Here, genes specifying two cyanobacterial Flvs have been expressed in the chloroplasts of Arabidopsis thaliana in an attempt to improve plant growth. Co-expression of Flv1 and Flv3 enhanced the efficiency of light utilization, boosting the plant’s capacity to accumulate biomass as the growth light intensity was raised. The Flv1/Flv3 transgenics displayed an increased production of ATP, an acceleration of carbohydrate metabolism and a more pronounced partitioning of sucrose into starch. The results suggest that Flvs are able to establish an efficient electron sink downstream of PSI, thereby ensuring efficient photosynthetic electron transport at moderate to high light intensities. The expression of Flvs thus acts to both protect photosynthesis and to control the ATP/NADPH ratio; together, their presence is beneficial for the plant’s growth potential.Fil: Tula, Suresh. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFil: Shahinnia, Fahimeh. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFil: Melzer, Michael. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFil: Rutten, Twan. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFil: Gómez, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Lodeyro, Anabella Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: von Wirén, Nicolaus. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Hajirezaei, Mohammad R.. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFrontiers Media2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184254Tula, Suresh; Shahinnia, Fahimeh; Melzer, Michael; Rutten, Twan; Gómez, Rodrigo; et al.; Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities; Frontiers Media; Frontiers in Plant Science; 11; 6-2020; 1-121664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fpls.2020.00902/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2020.00902info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:10:17Zoai:ri.conicet.gov.ar:11336/184254instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:10:17.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
title Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
spellingShingle Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
Tula, Suresh
A. THALIANA
BIOMASS
CYANOBACTERIA
ELECTRON SINK
FLAVODIIRON PROTEINS
PHOTOSYNTHESIS
PRIMARY METABOLISM
title_short Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
title_full Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
title_fullStr Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
title_full_unstemmed Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
title_sort Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities
dc.creator.none.fl_str_mv Tula, Suresh
Shahinnia, Fahimeh
Melzer, Michael
Rutten, Twan
Gómez, Rodrigo
Lodeyro, Anabella Fernanda
von Wirén, Nicolaus
Carrillo, Nestor Jose
Hajirezaei, Mohammad R.
author Tula, Suresh
author_facet Tula, Suresh
Shahinnia, Fahimeh
Melzer, Michael
Rutten, Twan
Gómez, Rodrigo
Lodeyro, Anabella Fernanda
von Wirén, Nicolaus
Carrillo, Nestor Jose
Hajirezaei, Mohammad R.
author_role author
author2 Shahinnia, Fahimeh
Melzer, Michael
Rutten, Twan
Gómez, Rodrigo
Lodeyro, Anabella Fernanda
von Wirén, Nicolaus
Carrillo, Nestor Jose
Hajirezaei, Mohammad R.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv A. THALIANA
BIOMASS
CYANOBACTERIA
ELECTRON SINK
FLAVODIIRON PROTEINS
PHOTOSYNTHESIS
PRIMARY METABOLISM
topic A. THALIANA
BIOMASS
CYANOBACTERIA
ELECTRON SINK
FLAVODIIRON PROTEINS
PHOTOSYNTHESIS
PRIMARY METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ability of plants to maintain photosynthesis in a dynamically changing environment is of central importance for their growth. As the photosynthetic machinery is a sensitive and early target of adverse environmental conditions as those typically found in the field, photosynthetic efficiency is not always optimal. Cyanobacteria, algae, mosses, liverworts and gymnosperms produce flavodiiron proteins (Flvs), a class of electron sinks not represented in angiosperms; these proteins act to mitigate the photoinhibition of photosystem I under high or fluctuating light. Here, genes specifying two cyanobacterial Flvs have been expressed in the chloroplasts of Arabidopsis thaliana in an attempt to improve plant growth. Co-expression of Flv1 and Flv3 enhanced the efficiency of light utilization, boosting the plant’s capacity to accumulate biomass as the growth light intensity was raised. The Flv1/Flv3 transgenics displayed an increased production of ATP, an acceleration of carbohydrate metabolism and a more pronounced partitioning of sucrose into starch. The results suggest that Flvs are able to establish an efficient electron sink downstream of PSI, thereby ensuring efficient photosynthetic electron transport at moderate to high light intensities. The expression of Flvs thus acts to both protect photosynthesis and to control the ATP/NADPH ratio; together, their presence is beneficial for the plant’s growth potential.
Fil: Tula, Suresh. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Shahinnia, Fahimeh. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Melzer, Michael. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Rutten, Twan. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Gómez, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Lodeyro, Anabella Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: von Wirén, Nicolaus. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hajirezaei, Mohammad R.. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
description The ability of plants to maintain photosynthesis in a dynamically changing environment is of central importance for their growth. As the photosynthetic machinery is a sensitive and early target of adverse environmental conditions as those typically found in the field, photosynthetic efficiency is not always optimal. Cyanobacteria, algae, mosses, liverworts and gymnosperms produce flavodiiron proteins (Flvs), a class of electron sinks not represented in angiosperms; these proteins act to mitigate the photoinhibition of photosystem I under high or fluctuating light. Here, genes specifying two cyanobacterial Flvs have been expressed in the chloroplasts of Arabidopsis thaliana in an attempt to improve plant growth. Co-expression of Flv1 and Flv3 enhanced the efficiency of light utilization, boosting the plant’s capacity to accumulate biomass as the growth light intensity was raised. The Flv1/Flv3 transgenics displayed an increased production of ATP, an acceleration of carbohydrate metabolism and a more pronounced partitioning of sucrose into starch. The results suggest that Flvs are able to establish an efficient electron sink downstream of PSI, thereby ensuring efficient photosynthetic electron transport at moderate to high light intensities. The expression of Flvs thus acts to both protect photosynthesis and to control the ATP/NADPH ratio; together, their presence is beneficial for the plant’s growth potential.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184254
Tula, Suresh; Shahinnia, Fahimeh; Melzer, Michael; Rutten, Twan; Gómez, Rodrigo; et al.; Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities; Frontiers Media; Frontiers in Plant Science; 11; 6-2020; 1-12
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184254
identifier_str_mv Tula, Suresh; Shahinnia, Fahimeh; Melzer, Michael; Rutten, Twan; Gómez, Rodrigo; et al.; Providing an Additional Electron Sink by the Introduction of Cyanobacterial Flavodiirons Enhances Growth of A. thaliana Under Various Light Intensities; Frontiers Media; Frontiers in Plant Science; 11; 6-2020; 1-12
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fpls.2020.00902/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2020.00902
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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