Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification

Autores
Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; Guibert, Edgardo Elvio
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.
Fil: Fuller, Barry J.. University College London; Estados Unidos
Fil: Petrenko, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Somov, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Hepatocyte
Drying
Hipothermic Preservation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22155
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And VitrificationFuller, Barry J.Petrenko, Alexander Y.Rodriguez, Joaquin ValentinSomov, Alexander Y.Balaban, Cecilia LucíaGuibert, Edgardo ElvioHepatocyteDryingHipothermic Preservationhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.Fil: Fuller, Barry J.. University College London; Estados UnidosFil: Petrenko, Alexander Y.. Ukraine Academy of Sciences; UcraniaFil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Somov, Alexander Y.. Ukraine Academy of Sciences; UcraniaFil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCryo Letters2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/22155Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; et al.; Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification; Cryo Letters; Cryo-letters; 37; 4; 7-2016; 432-4520143-2044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/cryo/cryo/2016/00000037/00000004/art00013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:04Zoai:ri.conicet.gov.ar:11336/22155instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:04.606CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
title Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
spellingShingle Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
Fuller, Barry J.
Hepatocyte
Drying
Hipothermic Preservation
title_short Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
title_full Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
title_fullStr Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
title_full_unstemmed Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
title_sort Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification
dc.creator.none.fl_str_mv Fuller, Barry J.
Petrenko, Alexander Y.
Rodriguez, Joaquin Valentin
Somov, Alexander Y.
Balaban, Cecilia Lucía
Guibert, Edgardo Elvio
author Fuller, Barry J.
author_facet Fuller, Barry J.
Petrenko, Alexander Y.
Rodriguez, Joaquin Valentin
Somov, Alexander Y.
Balaban, Cecilia Lucía
Guibert, Edgardo Elvio
author_role author
author2 Petrenko, Alexander Y.
Rodriguez, Joaquin Valentin
Somov, Alexander Y.
Balaban, Cecilia Lucía
Guibert, Edgardo Elvio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hepatocyte
Drying
Hipothermic Preservation
topic Hepatocyte
Drying
Hipothermic Preservation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.
Fil: Fuller, Barry J.. University College London; Estados Unidos
Fil: Petrenko, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Somov, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22155
Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; et al.; Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification; Cryo Letters; Cryo-letters; 37; 4; 7-2016; 432-452
0143-2044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22155
identifier_str_mv Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; et al.; Biopreservation of Hepatocytes: Current Concepts on Hypothermic Preservation, Cryopreservation, And Vitrification; Cryo Letters; Cryo-letters; 37; 4; 7-2016; 432-452
0143-2044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/cryo/cryo/2016/00000037/00000004/art00013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
dc.publisher.none.fl_str_mv Cryo Letters
publisher.none.fl_str_mv Cryo Letters
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268771796910080
score 13.13397

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