A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications

Autores
Fontana, Diego Sebastian; Marsili, Federico Francisco; Garay, Ernesto Sergio; Battagliotti, Juan Manuel; Etcheverrigaray, Marina; Kratje, Ricardo Bertoldo; Prieto, Claudio
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rabies is a neglected disease with an estimated annual mortality of 55,000 human deaths, affecting mainly low-income countries. Over 95% of these cases result from virus transmission through the bite of infected dogs and for this reason there is a real need for a cheap and effective rabies veterinary vaccine to be used in mass vaccination campaigns. In this work, we describe the establishment of a simple platform for the production of a virus-like particles based rabies vaccine using mammalian cells and roller bottles as culture system. Adherent cells were cultured during more than 15 days and VLPs were continuously produced and secreted to the culture supernatant. Immunogenicity and protective efficacy of VLPs were tested through rabies virus neutralizing antibody test and NIH potency test. These viral particles induced high titer of long lasting neutralizing antibodies and protected mice against active virus challenge. Therefore, this development represents a promising platform for the production of a new generation and virus-free rabies vaccine candidate for veterinary applications.
Fil: Fontana, Diego Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Marsili, Federico Francisco. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Garay, Ernesto Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Battagliotti, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Etcheverrigaray, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina. Cellargen Biotech S.R.L.; Argentina
Materia
RABIES
ROLLER BOTTLE
VETERINARY VACCINE
VIRUS-LIKE PARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/139615
Acceder
id CONICETDig_cf3fb02657e1c361954656e3b154e605
oai_identifier_str oai:ri.conicet.gov.ar:11336/139615
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applicationsFontana, Diego SebastianMarsili, Federico FranciscoGaray, Ernesto SergioBattagliotti, Juan ManuelEtcheverrigaray, MarinaKratje, Ricardo BertoldoPrieto, ClaudioRABIESROLLER BOTTLEVETERINARY VACCINEVIRUS-LIKE PARTICLEShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Rabies is a neglected disease with an estimated annual mortality of 55,000 human deaths, affecting mainly low-income countries. Over 95% of these cases result from virus transmission through the bite of infected dogs and for this reason there is a real need for a cheap and effective rabies veterinary vaccine to be used in mass vaccination campaigns. In this work, we describe the establishment of a simple platform for the production of a virus-like particles based rabies vaccine using mammalian cells and roller bottles as culture system. Adherent cells were cultured during more than 15 days and VLPs were continuously produced and secreted to the culture supernatant. Immunogenicity and protective efficacy of VLPs were tested through rabies virus neutralizing antibody test and NIH potency test. These viral particles induced high titer of long lasting neutralizing antibodies and protected mice against active virus challenge. Therefore, this development represents a promising platform for the production of a new generation and virus-free rabies vaccine candidate for veterinary applications.Fil: Fontana, Diego Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; ArgentinaFil: Marsili, Federico Francisco. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; ArgentinaFil: Garay, Ernesto Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; ArgentinaFil: Battagliotti, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; ArgentinaFil: Etcheverrigaray, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina. Cellargen Biotech S.R.L.; ArgentinaElsevier2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139615Fontana, Diego Sebastian; Marsili, Federico Francisco; Garay, Ernesto Sergio; Battagliotti, Juan Manuel; Etcheverrigaray, Marina; et al.; A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 65; 8-2019; 70-750147-9571CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0147957119300785info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2019.04.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:24:54Zoai:ri.conicet.gov.ar:11336/139615instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:24:54.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
title A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
spellingShingle A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
Fontana, Diego Sebastian
RABIES
ROLLER BOTTLE
VETERINARY VACCINE
VIRUS-LIKE PARTICLES
title_short A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
title_full A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
title_fullStr A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
title_full_unstemmed A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
title_sort A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications
dc.creator.none.fl_str_mv Fontana, Diego Sebastian
Marsili, Federico Francisco
Garay, Ernesto Sergio
Battagliotti, Juan Manuel
Etcheverrigaray, Marina
Kratje, Ricardo Bertoldo
Prieto, Claudio
author Fontana, Diego Sebastian
author_facet Fontana, Diego Sebastian
Marsili, Federico Francisco
Garay, Ernesto Sergio
Battagliotti, Juan Manuel
Etcheverrigaray, Marina
Kratje, Ricardo Bertoldo
Prieto, Claudio
author_role author
author2 Marsili, Federico Francisco
Garay, Ernesto Sergio
Battagliotti, Juan Manuel
Etcheverrigaray, Marina
Kratje, Ricardo Bertoldo
Prieto, Claudio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv RABIES
ROLLER BOTTLE
VETERINARY VACCINE
VIRUS-LIKE PARTICLES
topic RABIES
ROLLER BOTTLE
VETERINARY VACCINE
VIRUS-LIKE PARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Rabies is a neglected disease with an estimated annual mortality of 55,000 human deaths, affecting mainly low-income countries. Over 95% of these cases result from virus transmission through the bite of infected dogs and for this reason there is a real need for a cheap and effective rabies veterinary vaccine to be used in mass vaccination campaigns. In this work, we describe the establishment of a simple platform for the production of a virus-like particles based rabies vaccine using mammalian cells and roller bottles as culture system. Adherent cells were cultured during more than 15 days and VLPs were continuously produced and secreted to the culture supernatant. Immunogenicity and protective efficacy of VLPs were tested through rabies virus neutralizing antibody test and NIH potency test. These viral particles induced high titer of long lasting neutralizing antibodies and protected mice against active virus challenge. Therefore, this development represents a promising platform for the production of a new generation and virus-free rabies vaccine candidate for veterinary applications.
Fil: Fontana, Diego Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Marsili, Federico Francisco. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Garay, Ernesto Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Battagliotti, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina
Fil: Etcheverrigaray, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico; Argentina. Cellargen Biotech S.R.L.; Argentina
description Rabies is a neglected disease with an estimated annual mortality of 55,000 human deaths, affecting mainly low-income countries. Over 95% of these cases result from virus transmission through the bite of infected dogs and for this reason there is a real need for a cheap and effective rabies veterinary vaccine to be used in mass vaccination campaigns. In this work, we describe the establishment of a simple platform for the production of a virus-like particles based rabies vaccine using mammalian cells and roller bottles as culture system. Adherent cells were cultured during more than 15 days and VLPs were continuously produced and secreted to the culture supernatant. Immunogenicity and protective efficacy of VLPs were tested through rabies virus neutralizing antibody test and NIH potency test. These viral particles induced high titer of long lasting neutralizing antibodies and protected mice against active virus challenge. Therefore, this development represents a promising platform for the production of a new generation and virus-free rabies vaccine candidate for veterinary applications.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139615
Fontana, Diego Sebastian; Marsili, Federico Francisco; Garay, Ernesto Sergio; Battagliotti, Juan Manuel; Etcheverrigaray, Marina; et al.; A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 65; 8-2019; 70-75
0147-9571
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139615
identifier_str_mv Fontana, Diego Sebastian; Marsili, Federico Francisco; Garay, Ernesto Sergio; Battagliotti, Juan Manuel; Etcheverrigaray, Marina; et al.; A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 65; 8-2019; 70-75
0147-9571
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0147957119300785
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2019.04.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781792182337536
score 12.982451

Publicaciones similares