Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate

Autores
Fontana, Diego Sebastian; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Prieto, Claudio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rabies is one of the most lethal infectious diseases in the world, with a mortality approaching 100%. Thereare between 60,000 and 70,000 reported annual deaths, but this is probably an underestimation. Despitethe fact that there are vaccines available for rabies, there is a real need of developing more efficaciousand cheaper vaccines. This is particularly true for veterinary vaccines because dogs are still the mainvector for rabies transmission to human beings. In a previous work, we described the development andcharacterization of rabies virus-like particles (RV-VLPs) expressed in HEK293 cells. We showed that RV-VLPs are able to induce a specific antibodies response. In this work, we show that VLPs are able to protectmice against virus challenge. Furthermore, we developed a VLPs expressing HEK-293 clone (sP2E5) thatgrows in serum free medium (SFM) reaching high cell densities. sP2E5 was cultured in perfusion mode ina 5 L bioreactor for 20 days, and the RV-VLPs produced were capable of triggering a protective immuneresponse without the need of concentration or adjuvant addition. Further, these VLPs are able to inducethe production of rabies virus neutralizing antibodies. These results demonstrate that RV-VLPs are apromising rabies vaccine candidate.
Fil: Fontana, Diego Sebastian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Etcheverrigaray, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Materia
Rabies
Vaccine
Virus-Like Particles
Hek293 Cells
Serum Free Media
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44503
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44503
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidateFontana, Diego SebastianKratje, Ricardo BertoldoEtcheverrigaray, MarinaPrieto, ClaudioRabiesVaccineVirus-Like ParticlesHek293 CellsSerum Free Mediahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rabies is one of the most lethal infectious diseases in the world, with a mortality approaching 100%. Thereare between 60,000 and 70,000 reported annual deaths, but this is probably an underestimation. Despitethe fact that there are vaccines available for rabies, there is a real need of developing more efficaciousand cheaper vaccines. This is particularly true for veterinary vaccines because dogs are still the mainvector for rabies transmission to human beings. In a previous work, we described the development andcharacterization of rabies virus-like particles (RV-VLPs) expressed in HEK293 cells. We showed that RV-VLPs are able to induce a specific antibodies response. In this work, we show that VLPs are able to protectmice against virus challenge. Furthermore, we developed a VLPs expressing HEK-293 clone (sP2E5) thatgrows in serum free medium (SFM) reaching high cell densities. sP2E5 was cultured in perfusion mode ina 5 L bioreactor for 20 days, and the RV-VLPs produced were capable of triggering a protective immuneresponse without the need of concentration or adjuvant addition. Further, these VLPs are able to inducethe production of rabies virus neutralizing antibodies. These results demonstrate that RV-VLPs are apromising rabies vaccine candidate.Fil: Fontana, Diego Sebastian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Etcheverrigaray, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaElsevier2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44503Fontana, Diego Sebastian; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Prieto, Claudio; Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate; Elsevier; Vaccine; 33; 35; 8-2015; 4238-42460264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2015.03.088info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:35Zoai:ri.conicet.gov.ar:11336/44503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:35.58CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
title Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
spellingShingle Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
Fontana, Diego Sebastian
Rabies
Vaccine
Virus-Like Particles
Hek293 Cells
Serum Free Media
title_short Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
title_full Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
title_fullStr Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
title_full_unstemmed Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
title_sort Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate
dc.creator.none.fl_str_mv Fontana, Diego Sebastian
Kratje, Ricardo Bertoldo
Etcheverrigaray, Marina
Prieto, Claudio
author Fontana, Diego Sebastian
author_facet Fontana, Diego Sebastian
Kratje, Ricardo Bertoldo
Etcheverrigaray, Marina
Prieto, Claudio
author_role author
author2 Kratje, Ricardo Bertoldo
Etcheverrigaray, Marina
Prieto, Claudio
author2_role author
author
author
dc.subject.none.fl_str_mv Rabies
Vaccine
Virus-Like Particles
Hek293 Cells
Serum Free Media
topic Rabies
Vaccine
Virus-Like Particles
Hek293 Cells
Serum Free Media
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rabies is one of the most lethal infectious diseases in the world, with a mortality approaching 100%. Thereare between 60,000 and 70,000 reported annual deaths, but this is probably an underestimation. Despitethe fact that there are vaccines available for rabies, there is a real need of developing more efficaciousand cheaper vaccines. This is particularly true for veterinary vaccines because dogs are still the mainvector for rabies transmission to human beings. In a previous work, we described the development andcharacterization of rabies virus-like particles (RV-VLPs) expressed in HEK293 cells. We showed that RV-VLPs are able to induce a specific antibodies response. In this work, we show that VLPs are able to protectmice against virus challenge. Furthermore, we developed a VLPs expressing HEK-293 clone (sP2E5) thatgrows in serum free medium (SFM) reaching high cell densities. sP2E5 was cultured in perfusion mode ina 5 L bioreactor for 20 days, and the RV-VLPs produced were capable of triggering a protective immuneresponse without the need of concentration or adjuvant addition. Further, these VLPs are able to inducethe production of rabies virus neutralizing antibodies. These results demonstrate that RV-VLPs are apromising rabies vaccine candidate.
Fil: Fontana, Diego Sebastian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Etcheverrigaray, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
description Rabies is one of the most lethal infectious diseases in the world, with a mortality approaching 100%. Thereare between 60,000 and 70,000 reported annual deaths, but this is probably an underestimation. Despitethe fact that there are vaccines available for rabies, there is a real need of developing more efficaciousand cheaper vaccines. This is particularly true for veterinary vaccines because dogs are still the mainvector for rabies transmission to human beings. In a previous work, we described the development andcharacterization of rabies virus-like particles (RV-VLPs) expressed in HEK293 cells. We showed that RV-VLPs are able to induce a specific antibodies response. In this work, we show that VLPs are able to protectmice against virus challenge. Furthermore, we developed a VLPs expressing HEK-293 clone (sP2E5) thatgrows in serum free medium (SFM) reaching high cell densities. sP2E5 was cultured in perfusion mode ina 5 L bioreactor for 20 days, and the RV-VLPs produced were capable of triggering a protective immuneresponse without the need of concentration or adjuvant addition. Further, these VLPs are able to inducethe production of rabies virus neutralizing antibodies. These results demonstrate that RV-VLPs are apromising rabies vaccine candidate.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44503
Fontana, Diego Sebastian; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Prieto, Claudio; Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate; Elsevier; Vaccine; 33; 35; 8-2015; 4238-4246
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44503
identifier_str_mv Fontana, Diego Sebastian; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Prieto, Claudio; Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate; Elsevier; Vaccine; 33; 35; 8-2015; 4238-4246
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2015.03.088
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981069457981440
score 12.48226

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