Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length
- Autores
- Augusto, Marcelo T.; Hollmann, Axel; Porotto, Matteo; Moscona, Anne; Santos, Nuno C.
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A set of lipopeptides was recently reported for their broad-spectrum antiviral activity against viruses belonging to the Paramyxoviridae family, including human parainfluenza virus type 3 and Nipah virus. Among them, the peptide with a 24-unit PEG linker connecting it to a cholesterol moiety (VG-PEG24-Chol) was found to be the best membrane fusion inhibitory peptide. Here, we evaluated the interaction of the same set of peptides with biomembrane model systems and isolated human peripheral blood mononuclear cells (PBMC). VG-PEG24-Chol showed the highest insertion rate and it was among the peptides that induced a larger change on the surface pressure of cholesterol rich membranes. This peptide also displayed a high affinity towards PBMC membranes. These data provide new information about the dynamics of peptide-membrane interactions of a specific group of antiviral peptides, known for their potential as multipotent paramyxovirus antivirals.
Fil: Augusto, Marcelo T.. Universidade de Lisboa. Instituto de Medicina Molecular; Portugal
Fil: Hollmann, Axel. Universidade de Lisboa. Instituto de Medicina Molecular; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Porotto, Matteo. Columbia University Medical Center; Estados Unidos
Fil: Moscona, Anne. Columbia University Medical Center; Estados Unidos
Fil: Santos, Nuno C.. Universidade de Lisboa. Instituto de Medicina Molecular; Portugal - Materia
-
PARAMYXOVIRUSES
PEPTIDES
ANTIVIRAL
CHOLESTEROL
MEMBRANES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/40899
Ver los metadatos del registro completo
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Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker LengthAugusto, Marcelo T.Hollmann, AxelPorotto, MatteoMoscona, AnneSantos, Nuno C.PARAMYXOVIRUSESPEPTIDESANTIVIRALCHOLESTEROLMEMBRANEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A set of lipopeptides was recently reported for their broad-spectrum antiviral activity against viruses belonging to the Paramyxoviridae family, including human parainfluenza virus type 3 and Nipah virus. Among them, the peptide with a 24-unit PEG linker connecting it to a cholesterol moiety (VG-PEG24-Chol) was found to be the best membrane fusion inhibitory peptide. Here, we evaluated the interaction of the same set of peptides with biomembrane model systems and isolated human peripheral blood mononuclear cells (PBMC). VG-PEG24-Chol showed the highest insertion rate and it was among the peptides that induced a larger change on the surface pressure of cholesterol rich membranes. This peptide also displayed a high affinity towards PBMC membranes. These data provide new information about the dynamics of peptide-membrane interactions of a specific group of antiviral peptides, known for their potential as multipotent paramyxovirus antivirals.Fil: Augusto, Marcelo T.. Universidade de Lisboa. Instituto de Medicina Molecular; PortugalFil: Hollmann, Axel. Universidade de Lisboa. Instituto de Medicina Molecular; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Porotto, Matteo. Columbia University Medical Center; Estados UnidosFil: Moscona, Anne. Columbia University Medical Center; Estados UnidosFil: Santos, Nuno C.. Universidade de Lisboa. Instituto de Medicina Molecular; PortugalMolecular Diversity Preservation International2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40899Augusto, Marcelo T.; Hollmann, Axel; Porotto, Matteo; Moscona, Anne; Santos, Nuno C.; Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length; Molecular Diversity Preservation International; Molecules; 22; 7; 7-20171420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/22/7/1190info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules22071190info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:55Zoai:ri.conicet.gov.ar:11336/40899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:55.347CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length |
title |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length |
spellingShingle |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length Augusto, Marcelo T. PARAMYXOVIRUSES PEPTIDES ANTIVIRAL CHOLESTEROL MEMBRANES |
title_short |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length |
title_full |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length |
title_fullStr |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length |
title_full_unstemmed |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length |
title_sort |
Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length |
dc.creator.none.fl_str_mv |
Augusto, Marcelo T. Hollmann, Axel Porotto, Matteo Moscona, Anne Santos, Nuno C. |
author |
Augusto, Marcelo T. |
author_facet |
Augusto, Marcelo T. Hollmann, Axel Porotto, Matteo Moscona, Anne Santos, Nuno C. |
author_role |
author |
author2 |
Hollmann, Axel Porotto, Matteo Moscona, Anne Santos, Nuno C. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
PARAMYXOVIRUSES PEPTIDES ANTIVIRAL CHOLESTEROL MEMBRANES |
topic |
PARAMYXOVIRUSES PEPTIDES ANTIVIRAL CHOLESTEROL MEMBRANES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A set of lipopeptides was recently reported for their broad-spectrum antiviral activity against viruses belonging to the Paramyxoviridae family, including human parainfluenza virus type 3 and Nipah virus. Among them, the peptide with a 24-unit PEG linker connecting it to a cholesterol moiety (VG-PEG24-Chol) was found to be the best membrane fusion inhibitory peptide. Here, we evaluated the interaction of the same set of peptides with biomembrane model systems and isolated human peripheral blood mononuclear cells (PBMC). VG-PEG24-Chol showed the highest insertion rate and it was among the peptides that induced a larger change on the surface pressure of cholesterol rich membranes. This peptide also displayed a high affinity towards PBMC membranes. These data provide new information about the dynamics of peptide-membrane interactions of a specific group of antiviral peptides, known for their potential as multipotent paramyxovirus antivirals. Fil: Augusto, Marcelo T.. Universidade de Lisboa. Instituto de Medicina Molecular; Portugal Fil: Hollmann, Axel. Universidade de Lisboa. Instituto de Medicina Molecular; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Quilmes; Argentina Fil: Porotto, Matteo. Columbia University Medical Center; Estados Unidos Fil: Moscona, Anne. Columbia University Medical Center; Estados Unidos Fil: Santos, Nuno C.. Universidade de Lisboa. Instituto de Medicina Molecular; Portugal |
description |
A set of lipopeptides was recently reported for their broad-spectrum antiviral activity against viruses belonging to the Paramyxoviridae family, including human parainfluenza virus type 3 and Nipah virus. Among them, the peptide with a 24-unit PEG linker connecting it to a cholesterol moiety (VG-PEG24-Chol) was found to be the best membrane fusion inhibitory peptide. Here, we evaluated the interaction of the same set of peptides with biomembrane model systems and isolated human peripheral blood mononuclear cells (PBMC). VG-PEG24-Chol showed the highest insertion rate and it was among the peptides that induced a larger change on the surface pressure of cholesterol rich membranes. This peptide also displayed a high affinity towards PBMC membranes. These data provide new information about the dynamics of peptide-membrane interactions of a specific group of antiviral peptides, known for their potential as multipotent paramyxovirus antivirals. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/40899 Augusto, Marcelo T.; Hollmann, Axel; Porotto, Matteo; Moscona, Anne; Santos, Nuno C.; Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length; Molecular Diversity Preservation International; Molecules; 22; 7; 7-2017 1420-3049 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/40899 |
identifier_str_mv |
Augusto, Marcelo T.; Hollmann, Axel; Porotto, Matteo; Moscona, Anne; Santos, Nuno C.; Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length; Molecular Diversity Preservation International; Molecules; 22; 7; 7-2017 1420-3049 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/22/7/1190 info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules22071190 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613294127579136 |
score |
13.070432 |