Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay

Autores
Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.
Fil: Castro, E. R.. No especifíca;
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arbiza, Juan. Universidad de la Republica; Uruguay
Materia
GENETIC CHARACTERIZATION
PHYLOGENETIC ANALYSIS
PIGEON PARAMYXOVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195246
Acceder
id CONICETDig_ae0d11923ea3d7c1cb138ad92ca323b4
oai_identifier_str oai:ri.conicet.gov.ar:11336/195246
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in UruguayCastro, E. R.Zanetti, Flavia AdrianaArbiza, JuanGENETIC CHARACTERIZATIONPHYLOGENETIC ANALYSISPIGEON PARAMYXOVIRUShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.Fil: Castro, E. R.. No especifíca;Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arbiza, Juan. Universidad de la Republica; UruguayAmerican Association of Avian Pathologists2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195246Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan; Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay; American Association of Avian Pathologists; Avian Diseases; 56; 1; 4-2012; 243-2480005-2086CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1637/9835-061611-Case.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:25:17Zoai:ri.conicet.gov.ar:11336/195246instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:25:18.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
title Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
spellingShingle Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
Castro, E. R.
GENETIC CHARACTERIZATION
PHYLOGENETIC ANALYSIS
PIGEON PARAMYXOVIRUS
title_short Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
title_full Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
title_fullStr Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
title_full_unstemmed Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
title_sort Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
dc.creator.none.fl_str_mv Castro, E. R.
Zanetti, Flavia Adriana
Arbiza, Juan
author Castro, E. R.
author_facet Castro, E. R.
Zanetti, Flavia Adriana
Arbiza, Juan
author_role author
author2 Zanetti, Flavia Adriana
Arbiza, Juan
author2_role author
author
dc.subject.none.fl_str_mv GENETIC CHARACTERIZATION
PHYLOGENETIC ANALYSIS
PIGEON PARAMYXOVIRUS
topic GENETIC CHARACTERIZATION
PHYLOGENETIC ANALYSIS
PIGEON PARAMYXOVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.
Fil: Castro, E. R.. No especifíca;
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arbiza, Juan. Universidad de la Republica; Uruguay
description The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195246
Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan; Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay; American Association of Avian Pathologists; Avian Diseases; 56; 1; 4-2012; 243-248
0005-2086
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195246
identifier_str_mv Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan; Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay; American Association of Avian Pathologists; Avian Diseases; 56; 1; 4-2012; 243-248
0005-2086
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1637/9835-061611-Case.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Avian Pathologists
publisher.none.fl_str_mv American Association of Avian Pathologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083398050775040
score 13.22299

Publicaciones similares