Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
- Autores
- Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.
Fil: Castro, E. R.. No especifíca;
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arbiza, Juan. Universidad de la Republica; Uruguay - Materia
-
GENETIC CHARACTERIZATION
PHYLOGENETIC ANALYSIS
PIGEON PARAMYXOVIRUS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/195246
Ver los metadatos del registro completo
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spelling |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in UruguayCastro, E. R.Zanetti, Flavia AdrianaArbiza, JuanGENETIC CHARACTERIZATIONPHYLOGENETIC ANALYSISPIGEON PARAMYXOVIRUShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.Fil: Castro, E. R.. No especifíca;Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arbiza, Juan. Universidad de la Republica; UruguayAmerican Association of Avian Pathologists2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195246Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan; Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay; American Association of Avian Pathologists; Avian Diseases; 56; 1; 4-2012; 243-2480005-2086CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1637/9835-061611-Case.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:14Zoai:ri.conicet.gov.ar:11336/195246instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:14.938CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay |
title |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay |
spellingShingle |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay Castro, E. R. GENETIC CHARACTERIZATION PHYLOGENETIC ANALYSIS PIGEON PARAMYXOVIRUS |
title_short |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay |
title_full |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay |
title_fullStr |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay |
title_full_unstemmed |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay |
title_sort |
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay |
dc.creator.none.fl_str_mv |
Castro, E. R. Zanetti, Flavia Adriana Arbiza, Juan |
author |
Castro, E. R. |
author_facet |
Castro, E. R. Zanetti, Flavia Adriana Arbiza, Juan |
author_role |
author |
author2 |
Zanetti, Flavia Adriana Arbiza, Juan |
author2_role |
author author |
dc.subject.none.fl_str_mv |
GENETIC CHARACTERIZATION PHYLOGENETIC ANALYSIS PIGEON PARAMYXOVIRUS |
topic |
GENETIC CHARACTERIZATION PHYLOGENETIC ANALYSIS PIGEON PARAMYXOVIRUS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses. Fil: Castro, E. R.. No especifíca; Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Arbiza, Juan. Universidad de la Republica; Uruguay |
description |
The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/195246 Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan; Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay; American Association of Avian Pathologists; Avian Diseases; 56; 1; 4-2012; 243-248 0005-2086 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/195246 |
identifier_str_mv |
Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan; Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay; American Association of Avian Pathologists; Avian Diseases; 56; 1; 4-2012; 243-248 0005-2086 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1637/9835-061611-Case.1 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Association of Avian Pathologists |
publisher.none.fl_str_mv |
American Association of Avian Pathologists |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614286181138432 |
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13.070432 |