Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study

Autores
Rivas, Maria Gabriela; Gennaro, Ana Maria
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We use electron paramagnetic resonance (EPR) with liposoluble spin labels in order to study the lipid structures obtained after Triton X-100 extraction of erythrocyte membranes. The apparent order profile in these detergent resistant membranes (DRM) is very similar to that of the parent membrane, although with higher absolute values, consistent with a liquid-ordered state. DRM could also be obtained from erythrocytes previously depleted in a 40% of their membrane cholesterol, in apparent opposition to the phenomenon of raft disruption reported by other authors. However, the protein profile of these samples showed important differences with that of DRM from untreated cells. The analysis of our results suggests that the effect of Triton X-100 on cholesterol depleted erythrocytes is limited to the solubilization of raft proteins, without disrupting the lipid matrix of DRM.
Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Materia
Drm
Membrane Rafts
Erythrocyte
Cholesterol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28574
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label studyRivas, Maria GabrielaGennaro, Ana MariaDrmMembrane RaftsErythrocyteCholesterolhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1We use electron paramagnetic resonance (EPR) with liposoluble spin labels in order to study the lipid structures obtained after Triton X-100 extraction of erythrocyte membranes. The apparent order profile in these detergent resistant membranes (DRM) is very similar to that of the parent membrane, although with higher absolute values, consistent with a liquid-ordered state. DRM could also be obtained from erythrocytes previously depleted in a 40% of their membrane cholesterol, in apparent opposition to the phenomenon of raft disruption reported by other authors. However, the protein profile of these samples showed important differences with that of DRM from untreated cells. The analysis of our results suggests that the effect of Triton X-100 on cholesterol depleted erythrocytes is limited to the solubilization of raft proteins, without disrupting the lipid matrix of DRM.Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; ArgentinaFil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaElsevier Ireland2003-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28574Rivas, Maria Gabriela; Gennaro, Ana Maria; Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study; Elsevier Ireland; Chemistry And Physics Of Lipids; 122; 12-2003; 165-1690009-3084CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2N-47RBBJF-3&_user=2975255&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000057395&_version=1&_urlVersion=0&_userid=2975255&md5=437073b3d2fc9d0a221ba2b5b719bec3info:eu-repo/semantics/altIdentifier/doi/10.1016/S0009-3084(02)00189-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:39Zoai:ri.conicet.gov.ar:11336/28574instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:39.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
title Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
spellingShingle Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
Rivas, Maria Gabriela
Drm
Membrane Rafts
Erythrocyte
Cholesterol
title_short Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
title_full Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
title_fullStr Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
title_full_unstemmed Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
title_sort Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
dc.creator.none.fl_str_mv Rivas, Maria Gabriela
Gennaro, Ana Maria
author Rivas, Maria Gabriela
author_facet Rivas, Maria Gabriela
Gennaro, Ana Maria
author_role author
author2 Gennaro, Ana Maria
author2_role author
dc.subject.none.fl_str_mv Drm
Membrane Rafts
Erythrocyte
Cholesterol
topic Drm
Membrane Rafts
Erythrocyte
Cholesterol
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We use electron paramagnetic resonance (EPR) with liposoluble spin labels in order to study the lipid structures obtained after Triton X-100 extraction of erythrocyte membranes. The apparent order profile in these detergent resistant membranes (DRM) is very similar to that of the parent membrane, although with higher absolute values, consistent with a liquid-ordered state. DRM could also be obtained from erythrocytes previously depleted in a 40% of their membrane cholesterol, in apparent opposition to the phenomenon of raft disruption reported by other authors. However, the protein profile of these samples showed important differences with that of DRM from untreated cells. The analysis of our results suggests that the effect of Triton X-100 on cholesterol depleted erythrocytes is limited to the solubilization of raft proteins, without disrupting the lipid matrix of DRM.
Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description We use electron paramagnetic resonance (EPR) with liposoluble spin labels in order to study the lipid structures obtained after Triton X-100 extraction of erythrocyte membranes. The apparent order profile in these detergent resistant membranes (DRM) is very similar to that of the parent membrane, although with higher absolute values, consistent with a liquid-ordered state. DRM could also be obtained from erythrocytes previously depleted in a 40% of their membrane cholesterol, in apparent opposition to the phenomenon of raft disruption reported by other authors. However, the protein profile of these samples showed important differences with that of DRM from untreated cells. The analysis of our results suggests that the effect of Triton X-100 on cholesterol depleted erythrocytes is limited to the solubilization of raft proteins, without disrupting the lipid matrix of DRM.
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28574
Rivas, Maria Gabriela; Gennaro, Ana Maria; Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study; Elsevier Ireland; Chemistry And Physics Of Lipids; 122; 12-2003; 165-169
0009-3084
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28574
identifier_str_mv Rivas, Maria Gabriela; Gennaro, Ana Maria; Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study; Elsevier Ireland; Chemistry And Physics Of Lipids; 122; 12-2003; 165-169
0009-3084
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2N-47RBBJF-3&_user=2975255&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000057395&_version=1&_urlVersion=0&_userid=2975255&md5=437073b3d2fc9d0a221ba2b5b719bec3
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0009-3084(02)00189-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269533130194944
score 13.13397

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