Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis

Autores
Cianchino, Valeria Andrea; Acosta, Maria Gimena; Ortega, Claudia Alicia; Martinez, Luis Dante; Gomez, Maria Roxana Anabel
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Four different phytopharmaceutical dosage forms for use in weight control programs were analyzed. Two different ground herbal blends and their correspondent infusions, a capsule and a tincture were investigated for the presence of compounds used as adulterants in these products. A capillary electrophoresis (CE) method was developed and validated. The optimized experimental conditions were: BGE, sodium tetraborate buffer 20 mM, pH 9.2, voltage applied 30 kV, capillary temperature 25 °C, injection sample at 0.5 Psi during 5 s. Ephedrine, norephedrine, caffeine and furosemide were baseline separated in less than 7 min; the migration times were found to be 2.65, 2.90, 3.75 and 6.58 min, respectively. The analysis showed in sample 3 concentrations of 0.45 ± 0.03 mg g-1 (ephedrine), 0.33 ± 0.02 mg g-1 (norephedrine), 1.09 ± 0.41 mg g-1 (caffeine) and 0.80 ± 0.17 mg g-1 (furosemide). Caffeine content in samples 1, 2 and 4 was 0.61 ± 0.06 mg g-1, 15.66 ± 1.05 mg g-1 and 2.27 ± 0.13 mg ml-1, respectively. Linearity was obtained in the concentration range of 1-1000 μg ml-1. Limits of detection (LOD) and quantification (LOQ) were determined as 0.42 μg ml-1 and 1.40 μg ml-1 (ephedrine), 0.47 μg ml-1 and 1.40 μg ml-1 (norephedrine), 0.12 μg ml-1 and 0.48 μg ml-1 (caffeine), 0.22 μg ml-1 and 0.73 μg ml-1 (furosemide). The common constituents of the samples did not interfere with the potential adulterants. Repeatability was better than 0.24% RSD for the retention time and 1.43% for the peak area. Intermediate precision was tested by changing the capillary, the day of operation and the operator, in all the cases the %RSD was better than 3.06. © 2007 Elsevier Ltd. All rights reserved.
Fil: Cianchino, Valeria Andrea. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Ortega, Claudia Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
ADULTERANTS
CAPILLARY ELECTROPHORESIS
DIETARY SUPPLEMENTS
QUALITY CONTROL
WEIGHT CONTROL DRUGS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92823
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92823
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresisCianchino, Valeria AndreaAcosta, Maria GimenaOrtega, Claudia AliciaMartinez, Luis DanteGomez, Maria Roxana AnabelADULTERANTSCAPILLARY ELECTROPHORESISDIETARY SUPPLEMENTSQUALITY CONTROLWEIGHT CONTROL DRUGShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Four different phytopharmaceutical dosage forms for use in weight control programs were analyzed. Two different ground herbal blends and their correspondent infusions, a capsule and a tincture were investigated for the presence of compounds used as adulterants in these products. A capillary electrophoresis (CE) method was developed and validated. The optimized experimental conditions were: BGE, sodium tetraborate buffer 20 mM, pH 9.2, voltage applied 30 kV, capillary temperature 25 °C, injection sample at 0.5 Psi during 5 s. Ephedrine, norephedrine, caffeine and furosemide were baseline separated in less than 7 min; the migration times were found to be 2.65, 2.90, 3.75 and 6.58 min, respectively. The analysis showed in sample 3 concentrations of 0.45 ± 0.03 mg g-1 (ephedrine), 0.33 ± 0.02 mg g-1 (norephedrine), 1.09 ± 0.41 mg g-1 (caffeine) and 0.80 ± 0.17 mg g-1 (furosemide). Caffeine content in samples 1, 2 and 4 was 0.61 ± 0.06 mg g-1, 15.66 ± 1.05 mg g-1 and 2.27 ± 0.13 mg ml-1, respectively. Linearity was obtained in the concentration range of 1-1000 μg ml-1. Limits of detection (LOD) and quantification (LOQ) were determined as 0.42 μg ml-1 and 1.40 μg ml-1 (ephedrine), 0.47 μg ml-1 and 1.40 μg ml-1 (norephedrine), 0.12 μg ml-1 and 0.48 μg ml-1 (caffeine), 0.22 μg ml-1 and 0.73 μg ml-1 (furosemide). The common constituents of the samples did not interfere with the potential adulterants. Repeatability was better than 0.24% RSD for the retention time and 1.43% for the peak area. Intermediate precision was tested by changing the capillary, the day of operation and the operator, in all the cases the %RSD was better than 3.06. © 2007 Elsevier Ltd. All rights reserved.Fil: Cianchino, Valeria Andrea. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Ortega, Claudia Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier2008-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92823Cianchino, Valeria Andrea; Acosta, Maria Gimena; Ortega, Claudia Alicia; Martinez, Luis Dante; Gomez, Maria Roxana Anabel; Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis; Elsevier; Food Chemistry; 108; 3; 6-2008; 1075-10810308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2007.11.042info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814607012010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:12Zoai:ri.conicet.gov.ar:11336/92823instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:12.381CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
title Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
spellingShingle Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
Cianchino, Valeria Andrea
ADULTERANTS
CAPILLARY ELECTROPHORESIS
DIETARY SUPPLEMENTS
QUALITY CONTROL
WEIGHT CONTROL DRUGS
title_short Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
title_full Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
title_fullStr Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
title_full_unstemmed Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
title_sort Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis
dc.creator.none.fl_str_mv Cianchino, Valeria Andrea
Acosta, Maria Gimena
Ortega, Claudia Alicia
Martinez, Luis Dante
Gomez, Maria Roxana Anabel
author Cianchino, Valeria Andrea
author_facet Cianchino, Valeria Andrea
Acosta, Maria Gimena
Ortega, Claudia Alicia
Martinez, Luis Dante
Gomez, Maria Roxana Anabel
author_role author
author2 Acosta, Maria Gimena
Ortega, Claudia Alicia
Martinez, Luis Dante
Gomez, Maria Roxana Anabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv ADULTERANTS
CAPILLARY ELECTROPHORESIS
DIETARY SUPPLEMENTS
QUALITY CONTROL
WEIGHT CONTROL DRUGS
topic ADULTERANTS
CAPILLARY ELECTROPHORESIS
DIETARY SUPPLEMENTS
QUALITY CONTROL
WEIGHT CONTROL DRUGS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Four different phytopharmaceutical dosage forms for use in weight control programs were analyzed. Two different ground herbal blends and their correspondent infusions, a capsule and a tincture were investigated for the presence of compounds used as adulterants in these products. A capillary electrophoresis (CE) method was developed and validated. The optimized experimental conditions were: BGE, sodium tetraborate buffer 20 mM, pH 9.2, voltage applied 30 kV, capillary temperature 25 °C, injection sample at 0.5 Psi during 5 s. Ephedrine, norephedrine, caffeine and furosemide were baseline separated in less than 7 min; the migration times were found to be 2.65, 2.90, 3.75 and 6.58 min, respectively. The analysis showed in sample 3 concentrations of 0.45 ± 0.03 mg g-1 (ephedrine), 0.33 ± 0.02 mg g-1 (norephedrine), 1.09 ± 0.41 mg g-1 (caffeine) and 0.80 ± 0.17 mg g-1 (furosemide). Caffeine content in samples 1, 2 and 4 was 0.61 ± 0.06 mg g-1, 15.66 ± 1.05 mg g-1 and 2.27 ± 0.13 mg ml-1, respectively. Linearity was obtained in the concentration range of 1-1000 μg ml-1. Limits of detection (LOD) and quantification (LOQ) were determined as 0.42 μg ml-1 and 1.40 μg ml-1 (ephedrine), 0.47 μg ml-1 and 1.40 μg ml-1 (norephedrine), 0.12 μg ml-1 and 0.48 μg ml-1 (caffeine), 0.22 μg ml-1 and 0.73 μg ml-1 (furosemide). The common constituents of the samples did not interfere with the potential adulterants. Repeatability was better than 0.24% RSD for the retention time and 1.43% for the peak area. Intermediate precision was tested by changing the capillary, the day of operation and the operator, in all the cases the %RSD was better than 3.06. © 2007 Elsevier Ltd. All rights reserved.
Fil: Cianchino, Valeria Andrea. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Ortega, Claudia Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description Four different phytopharmaceutical dosage forms for use in weight control programs were analyzed. Two different ground herbal blends and their correspondent infusions, a capsule and a tincture were investigated for the presence of compounds used as adulterants in these products. A capillary electrophoresis (CE) method was developed and validated. The optimized experimental conditions were: BGE, sodium tetraborate buffer 20 mM, pH 9.2, voltage applied 30 kV, capillary temperature 25 °C, injection sample at 0.5 Psi during 5 s. Ephedrine, norephedrine, caffeine and furosemide were baseline separated in less than 7 min; the migration times were found to be 2.65, 2.90, 3.75 and 6.58 min, respectively. The analysis showed in sample 3 concentrations of 0.45 ± 0.03 mg g-1 (ephedrine), 0.33 ± 0.02 mg g-1 (norephedrine), 1.09 ± 0.41 mg g-1 (caffeine) and 0.80 ± 0.17 mg g-1 (furosemide). Caffeine content in samples 1, 2 and 4 was 0.61 ± 0.06 mg g-1, 15.66 ± 1.05 mg g-1 and 2.27 ± 0.13 mg ml-1, respectively. Linearity was obtained in the concentration range of 1-1000 μg ml-1. Limits of detection (LOD) and quantification (LOQ) were determined as 0.42 μg ml-1 and 1.40 μg ml-1 (ephedrine), 0.47 μg ml-1 and 1.40 μg ml-1 (norephedrine), 0.12 μg ml-1 and 0.48 μg ml-1 (caffeine), 0.22 μg ml-1 and 0.73 μg ml-1 (furosemide). The common constituents of the samples did not interfere with the potential adulterants. Repeatability was better than 0.24% RSD for the retention time and 1.43% for the peak area. Intermediate precision was tested by changing the capillary, the day of operation and the operator, in all the cases the %RSD was better than 3.06. © 2007 Elsevier Ltd. All rights reserved.
publishDate 2008
dc.date.none.fl_str_mv 2008-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92823
Cianchino, Valeria Andrea; Acosta, Maria Gimena; Ortega, Claudia Alicia; Martinez, Luis Dante; Gomez, Maria Roxana Anabel; Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis; Elsevier; Food Chemistry; 108; 3; 6-2008; 1075-1081
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92823
identifier_str_mv Cianchino, Valeria Andrea; Acosta, Maria Gimena; Ortega, Claudia Alicia; Martinez, Luis Dante; Gomez, Maria Roxana Anabel; Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis; Elsevier; Food Chemistry; 108; 3; 6-2008; 1075-1081
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2007.11.042
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814607012010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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