Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals

Autores
Sombra, Lorena Luján; Gomez, Maria Roxana Anabel; Olsina, Roberto Antonio; Martinez, Luis Dante; Silva, María Fernanda
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The last years have seen a significant increase in the use of herbal medicines and their preparations all over the world. Adulterations with synthetic drugs are common problems with phytopharmaceutical products and this can potentially cause adverse effects. In consequence, it is important to determine the presence of synthetic drugs in herbal medicines to ensure their efficacy and safety. In this study, guarana derivatives were analyzed by capillary electrophoresis (CE), and the results were compared with those obtained by the HPLC technique. In order to obtain adequate fingerprints, and search for adulterants, caffeine was used as the marker compound. This separation method was applied to analyze the seed powder and commercial tablets of Paulinia cupana Mart. The methodology performance was evaluated in terms of specificity, sensitivity and precision. The results are in agreement with those obtained by the HPLC method. Furthermore, the analysis time of the CE method is up to two times shorter than the respective parameter in HPLC and solvent consumption is more than 100-fold less. © 2004 Elsevier B.V. All rights reserved.
Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
ADULTERANTS
CAPILLARY ELECTROPHORESIS
GURANA
HERBAL MEDICINES
HPLC
PHYTOPHARMACEUTICALS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93546
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticalsSombra, Lorena LujánGomez, Maria Roxana AnabelOlsina, Roberto AntonioMartinez, Luis DanteSilva, María FernandaADULTERANTSCAPILLARY ELECTROPHORESISGURANAHERBAL MEDICINESHPLCPHYTOPHARMACEUTICALShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The last years have seen a significant increase in the use of herbal medicines and their preparations all over the world. Adulterations with synthetic drugs are common problems with phytopharmaceutical products and this can potentially cause adverse effects. In consequence, it is important to determine the presence of synthetic drugs in herbal medicines to ensure their efficacy and safety. In this study, guarana derivatives were analyzed by capillary electrophoresis (CE), and the results were compared with those obtained by the HPLC technique. In order to obtain adequate fingerprints, and search for adulterants, caffeine was used as the marker compound. This separation method was applied to analyze the seed powder and commercial tablets of Paulinia cupana Mart. The methodology performance was evaluated in terms of specificity, sensitivity and precision. The results are in agreement with those obtained by the HPLC method. Furthermore, the analysis time of the CE method is up to two times shorter than the respective parameter in HPLC and solvent consumption is more than 100-fold less. © 2004 Elsevier B.V. All rights reserved.Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; ArgentinaFil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; ArgentinaFil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science2005-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93546Sombra, Lorena Luján; Gomez, Maria Roxana Anabel; Olsina, Roberto Antonio; Martinez, Luis Dante; Silva, María Fernanda; Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals; Elsevier Science; Journal of Pharmaceutical and Biomedical Analysis; 36; 5; 1-2005; 989-9940731-7085CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpba.2004.08.026info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0731708504004091info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:51Zoai:ri.conicet.gov.ar:11336/93546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:52.198CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
title Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
spellingShingle Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
Sombra, Lorena Luján
ADULTERANTS
CAPILLARY ELECTROPHORESIS
GURANA
HERBAL MEDICINES
HPLC
PHYTOPHARMACEUTICALS
title_short Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
title_full Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
title_fullStr Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
title_full_unstemmed Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
title_sort Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals
dc.creator.none.fl_str_mv Sombra, Lorena Luján
Gomez, Maria Roxana Anabel
Olsina, Roberto Antonio
Martinez, Luis Dante
Silva, María Fernanda
author Sombra, Lorena Luján
author_facet Sombra, Lorena Luján
Gomez, Maria Roxana Anabel
Olsina, Roberto Antonio
Martinez, Luis Dante
Silva, María Fernanda
author_role author
author2 Gomez, Maria Roxana Anabel
Olsina, Roberto Antonio
Martinez, Luis Dante
Silva, María Fernanda
author2_role author
author
author
author
dc.subject.none.fl_str_mv ADULTERANTS
CAPILLARY ELECTROPHORESIS
GURANA
HERBAL MEDICINES
HPLC
PHYTOPHARMACEUTICALS
topic ADULTERANTS
CAPILLARY ELECTROPHORESIS
GURANA
HERBAL MEDICINES
HPLC
PHYTOPHARMACEUTICALS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The last years have seen a significant increase in the use of herbal medicines and their preparations all over the world. Adulterations with synthetic drugs are common problems with phytopharmaceutical products and this can potentially cause adverse effects. In consequence, it is important to determine the presence of synthetic drugs in herbal medicines to ensure their efficacy and safety. In this study, guarana derivatives were analyzed by capillary electrophoresis (CE), and the results were compared with those obtained by the HPLC technique. In order to obtain adequate fingerprints, and search for adulterants, caffeine was used as the marker compound. This separation method was applied to analyze the seed powder and commercial tablets of Paulinia cupana Mart. The methodology performance was evaluated in terms of specificity, sensitivity and precision. The results are in agreement with those obtained by the HPLC method. Furthermore, the analysis time of the CE method is up to two times shorter than the respective parameter in HPLC and solvent consumption is more than 100-fold less. © 2004 Elsevier B.V. All rights reserved.
Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina
Fil: Gomez, Maria Roxana Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description The last years have seen a significant increase in the use of herbal medicines and their preparations all over the world. Adulterations with synthetic drugs are common problems with phytopharmaceutical products and this can potentially cause adverse effects. In consequence, it is important to determine the presence of synthetic drugs in herbal medicines to ensure their efficacy and safety. In this study, guarana derivatives were analyzed by capillary electrophoresis (CE), and the results were compared with those obtained by the HPLC technique. In order to obtain adequate fingerprints, and search for adulterants, caffeine was used as the marker compound. This separation method was applied to analyze the seed powder and commercial tablets of Paulinia cupana Mart. The methodology performance was evaluated in terms of specificity, sensitivity and precision. The results are in agreement with those obtained by the HPLC method. Furthermore, the analysis time of the CE method is up to two times shorter than the respective parameter in HPLC and solvent consumption is more than 100-fold less. © 2004 Elsevier B.V. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93546
Sombra, Lorena Luján; Gomez, Maria Roxana Anabel; Olsina, Roberto Antonio; Martinez, Luis Dante; Silva, María Fernanda; Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals; Elsevier Science; Journal of Pharmaceutical and Biomedical Analysis; 36; 5; 1-2005; 989-994
0731-7085
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93546
identifier_str_mv Sombra, Lorena Luján; Gomez, Maria Roxana Anabel; Olsina, Roberto Antonio; Martinez, Luis Dante; Silva, María Fernanda; Comparative study between capillary electrophoresis and high performance liquid chromatography in "guarana" based phytopharmaceuticals; Elsevier Science; Journal of Pharmaceutical and Biomedical Analysis; 36; 5; 1-2005; 989-994
0731-7085
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpba.2004.08.026
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0731708504004091
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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