The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth

Autores
Hobecker, Karen Vanesa; Reynoso, Mauricio Alberto; Bustos Sanmamed, Pilar; Wen, Jiangqi; Mysore, Kirankumar S.; Crespi, Martín; Blanco, Flavio Antonio; Zanetti, María Eugenia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Legume roots form two types of postembryonic organs, lateral roots and symbiotic nodules. Nodule formation is the result of the interaction of legumes with rhizobia and requires the mitotic activation and differentiation of root cells as well as an independent, but coordinated, program that allows infection by rhizobia. MicroRNA390 (miR390) is an evolutionarily conserved microRNA that targets the Trans-Acting Short Interference RNA3 (TAS3) transcript. Cleavage of TAS3 by ARGONAUTE7 results in the production of trans-acting small interference RNAs, which target mRNAs encoding AUXIN RESPONSE FACTOR2 (ARF2), ARF3, and ARF4. Here, we show that activation of the miR390/TAS3 regulatory module by overexpression of miR390 in Medicago truncatula promotes lateral root growth but prevents nodule organogenesis, rhizobial infection, and the induction of two key nodulation genes, Nodulation Signaling Pathway1 (NSP1) and NSP2. Accordingly, inactivation of the miR390/TAS3 module, either by expression of a miR390 target mimicry construct or mutations in ARGONAUTE7, enhances nodulation and rhizobial infection, alters the spatial distribution of the nodules, and increases the percentage of nodules with multiple meristems. Our results revealed a key role of the miR390/TAS3 pathway in legumes as a modulator of lateral root organs, playing opposite roles in lateral root and nodule development.
Fil: Hobecker, Karen Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Bustos Sanmamed, Pilar. Université Paris Sud; Francia
Fil: Wen, Jiangqi. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Mysore, Kirankumar S.. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Crespi, Martín. Institute Of Plant Sciences Paris, Saclay; Francia
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
ARGONAUTE 7
Nitrogen fixation
Root architecture
Symbiosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65856
Acceder
id CONICETDig_cee97e9a18a5074da6a2577e02abcebe
oai_identifier_str oai:ri.conicet.gov.ar:11336/65856
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growthHobecker, Karen VanesaReynoso, Mauricio AlbertoBustos Sanmamed, PilarWen, JiangqiMysore, Kirankumar S.Crespi, MartínBlanco, Flavio AntonioZanetti, María EugeniaARGONAUTE 7Nitrogen fixationRoot architectureSymbiosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Legume roots form two types of postembryonic organs, lateral roots and symbiotic nodules. Nodule formation is the result of the interaction of legumes with rhizobia and requires the mitotic activation and differentiation of root cells as well as an independent, but coordinated, program that allows infection by rhizobia. MicroRNA390 (miR390) is an evolutionarily conserved microRNA that targets the Trans-Acting Short Interference RNA3 (TAS3) transcript. Cleavage of TAS3 by ARGONAUTE7 results in the production of trans-acting small interference RNAs, which target mRNAs encoding AUXIN RESPONSE FACTOR2 (ARF2), ARF3, and ARF4. Here, we show that activation of the miR390/TAS3 regulatory module by overexpression of miR390 in Medicago truncatula promotes lateral root growth but prevents nodule organogenesis, rhizobial infection, and the induction of two key nodulation genes, Nodulation Signaling Pathway1 (NSP1) and NSP2. Accordingly, inactivation of the miR390/TAS3 module, either by expression of a miR390 target mimicry construct or mutations in ARGONAUTE7, enhances nodulation and rhizobial infection, alters the spatial distribution of the nodules, and increases the percentage of nodules with multiple meristems. Our results revealed a key role of the miR390/TAS3 pathway in legumes as a modulator of lateral root organs, playing opposite roles in lateral root and nodule development.Fil: Hobecker, Karen Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Bustos Sanmamed, Pilar. Université Paris Sud; FranciaFil: Wen, Jiangqi. The Samuel Roberts Noble Foundation; Estados UnidosFil: Mysore, Kirankumar S.. The Samuel Roberts Noble Foundation; Estados UnidosFil: Crespi, Martín. Institute Of Plant Sciences Paris, Saclay; FranciaFil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAmerican Society of Plant Biologist2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65856Hobecker, Karen Vanesa; Reynoso, Mauricio Alberto; Bustos Sanmamed, Pilar; Wen, Jiangqi; Mysore, Kirankumar S.; et al.; The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth; American Society of Plant Biologist; Plant Physiology; 174; 4; 8-2017; 2469-24860032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/174/4/2469info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.17.00464info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:03Zoai:ri.conicet.gov.ar:11336/65856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:03.311CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
title The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
spellingShingle The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
Hobecker, Karen Vanesa
ARGONAUTE 7
Nitrogen fixation
Root architecture
Symbiosis
title_short The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
title_full The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
title_fullStr The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
title_full_unstemmed The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
title_sort The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth
dc.creator.none.fl_str_mv Hobecker, Karen Vanesa
Reynoso, Mauricio Alberto
Bustos Sanmamed, Pilar
Wen, Jiangqi
Mysore, Kirankumar S.
Crespi, Martín
Blanco, Flavio Antonio
Zanetti, María Eugenia
author Hobecker, Karen Vanesa
author_facet Hobecker, Karen Vanesa
Reynoso, Mauricio Alberto
Bustos Sanmamed, Pilar
Wen, Jiangqi
Mysore, Kirankumar S.
Crespi, Martín
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_role author
author2 Reynoso, Mauricio Alberto
Bustos Sanmamed, Pilar
Wen, Jiangqi
Mysore, Kirankumar S.
Crespi, Martín
Blanco, Flavio Antonio
Zanetti, María Eugenia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGONAUTE 7
Nitrogen fixation
Root architecture
Symbiosis
topic ARGONAUTE 7
Nitrogen fixation
Root architecture
Symbiosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Legume roots form two types of postembryonic organs, lateral roots and symbiotic nodules. Nodule formation is the result of the interaction of legumes with rhizobia and requires the mitotic activation and differentiation of root cells as well as an independent, but coordinated, program that allows infection by rhizobia. MicroRNA390 (miR390) is an evolutionarily conserved microRNA that targets the Trans-Acting Short Interference RNA3 (TAS3) transcript. Cleavage of TAS3 by ARGONAUTE7 results in the production of trans-acting small interference RNAs, which target mRNAs encoding AUXIN RESPONSE FACTOR2 (ARF2), ARF3, and ARF4. Here, we show that activation of the miR390/TAS3 regulatory module by overexpression of miR390 in Medicago truncatula promotes lateral root growth but prevents nodule organogenesis, rhizobial infection, and the induction of two key nodulation genes, Nodulation Signaling Pathway1 (NSP1) and NSP2. Accordingly, inactivation of the miR390/TAS3 module, either by expression of a miR390 target mimicry construct or mutations in ARGONAUTE7, enhances nodulation and rhizobial infection, alters the spatial distribution of the nodules, and increases the percentage of nodules with multiple meristems. Our results revealed a key role of the miR390/TAS3 pathway in legumes as a modulator of lateral root organs, playing opposite roles in lateral root and nodule development.
Fil: Hobecker, Karen Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Reynoso, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Bustos Sanmamed, Pilar. Université Paris Sud; Francia
Fil: Wen, Jiangqi. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Mysore, Kirankumar S.. The Samuel Roberts Noble Foundation; Estados Unidos
Fil: Crespi, Martín. Institute Of Plant Sciences Paris, Saclay; Francia
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Legume roots form two types of postembryonic organs, lateral roots and symbiotic nodules. Nodule formation is the result of the interaction of legumes with rhizobia and requires the mitotic activation and differentiation of root cells as well as an independent, but coordinated, program that allows infection by rhizobia. MicroRNA390 (miR390) is an evolutionarily conserved microRNA that targets the Trans-Acting Short Interference RNA3 (TAS3) transcript. Cleavage of TAS3 by ARGONAUTE7 results in the production of trans-acting small interference RNAs, which target mRNAs encoding AUXIN RESPONSE FACTOR2 (ARF2), ARF3, and ARF4. Here, we show that activation of the miR390/TAS3 regulatory module by overexpression of miR390 in Medicago truncatula promotes lateral root growth but prevents nodule organogenesis, rhizobial infection, and the induction of two key nodulation genes, Nodulation Signaling Pathway1 (NSP1) and NSP2. Accordingly, inactivation of the miR390/TAS3 module, either by expression of a miR390 target mimicry construct or mutations in ARGONAUTE7, enhances nodulation and rhizobial infection, alters the spatial distribution of the nodules, and increases the percentage of nodules with multiple meristems. Our results revealed a key role of the miR390/TAS3 pathway in legumes as a modulator of lateral root organs, playing opposite roles in lateral root and nodule development.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65856
Hobecker, Karen Vanesa; Reynoso, Mauricio Alberto; Bustos Sanmamed, Pilar; Wen, Jiangqi; Mysore, Kirankumar S.; et al.; The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth; American Society of Plant Biologist; Plant Physiology; 174; 4; 8-2017; 2469-2486
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65856
identifier_str_mv Hobecker, Karen Vanesa; Reynoso, Mauricio Alberto; Bustos Sanmamed, Pilar; Wen, Jiangqi; Mysore, Kirankumar S.; et al.; The microRNA390/TAS3 pathway mediates symbiotic nodulation and lateral root growth; American Society of Plant Biologist; Plant Physiology; 174; 4; 8-2017; 2469-2486
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/174/4/2469
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.17.00464
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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