A novel methodology for imaging newly bone formation around bioceramic bone substitutes

Autores
Gorustovich Alonso, Alejandro Adrian; Sivak, Matias Gabriel; Guglielmotti, Maria Beatriz
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, Frost's bulk‐staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol‐soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.
Fil: Gorustovich Alonso, Alejandro Adrian. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sivak, Matias Gabriel. Universidad de Buenos Aires; Argentina
Fil: Guglielmotti, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Materia
Bone Formation
Bioceramic Bone
Substitutes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78234
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel methodology for imaging newly bone formation around bioceramic bone substitutesGorustovich Alonso, Alejandro AdrianSivak, Matias GabrielGuglielmotti, Maria BeatrizBone FormationBioceramic BoneSubstituteshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In this study, Frost's bulk‐staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol‐soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.Fil: Gorustovich Alonso, Alejandro Adrian. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sivak, Matias Gabriel. Universidad de Buenos Aires; ArgentinaFil: Guglielmotti, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaWiley-liss, Div John Wiley & Sons Inc2006-11-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78234Gorustovich Alonso, Alejandro Adrian; Sivak, Matias Gabriel; Guglielmotti, Maria Beatriz; A novel methodology for imaging newly bone formation around bioceramic bone substitutes; Wiley-liss, Div John Wiley & Sons Inc; Journal of Biomedical Materials Research Part A; 81; 2; 21-11-2006; 443-4451549-3296CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.1002/jbm.a.31071info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.31071info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:21Zoai:ri.conicet.gov.ar:11336/78234instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:21.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel methodology for imaging newly bone formation around bioceramic bone substitutes
title A novel methodology for imaging newly bone formation around bioceramic bone substitutes
spellingShingle A novel methodology for imaging newly bone formation around bioceramic bone substitutes
Gorustovich Alonso, Alejandro Adrian
Bone Formation
Bioceramic Bone
Substitutes
title_short A novel methodology for imaging newly bone formation around bioceramic bone substitutes
title_full A novel methodology for imaging newly bone formation around bioceramic bone substitutes
title_fullStr A novel methodology for imaging newly bone formation around bioceramic bone substitutes
title_full_unstemmed A novel methodology for imaging newly bone formation around bioceramic bone substitutes
title_sort A novel methodology for imaging newly bone formation around bioceramic bone substitutes
dc.creator.none.fl_str_mv Gorustovich Alonso, Alejandro Adrian
Sivak, Matias Gabriel
Guglielmotti, Maria Beatriz
author Gorustovich Alonso, Alejandro Adrian
author_facet Gorustovich Alonso, Alejandro Adrian
Sivak, Matias Gabriel
Guglielmotti, Maria Beatriz
author_role author
author2 Sivak, Matias Gabriel
Guglielmotti, Maria Beatriz
author2_role author
author
dc.subject.none.fl_str_mv Bone Formation
Bioceramic Bone
Substitutes
topic Bone Formation
Bioceramic Bone
Substitutes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, Frost's bulk‐staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol‐soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.
Fil: Gorustovich Alonso, Alejandro Adrian. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sivak, Matias Gabriel. Universidad de Buenos Aires; Argentina
Fil: Guglielmotti, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
description In this study, Frost's bulk‐staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol‐soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.
publishDate 2006
dc.date.none.fl_str_mv 2006-11-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78234
Gorustovich Alonso, Alejandro Adrian; Sivak, Matias Gabriel; Guglielmotti, Maria Beatriz; A novel methodology for imaging newly bone formation around bioceramic bone substitutes; Wiley-liss, Div John Wiley & Sons Inc; Journal of Biomedical Materials Research Part A; 81; 2; 21-11-2006; 443-445
1549-3296
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78234
identifier_str_mv Gorustovich Alonso, Alejandro Adrian; Sivak, Matias Gabriel; Guglielmotti, Maria Beatriz; A novel methodology for imaging newly bone formation around bioceramic bone substitutes; Wiley-liss, Div John Wiley & Sons Inc; Journal of Biomedical Materials Research Part A; 81; 2; 21-11-2006; 443-445
1549-3296
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.1002/jbm.a.31071
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.31071
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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