Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
- Autores
- Zegbi, Sara; Sagüés, María Federica; Saumell, Carlos Alfredo; Iglesias, Lucía Emilia; Guerrero, Ines; Fernández, Alicia Silvina
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes.
Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Guerrero, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
27th Conference of the World Association for the Advancement of Veterinary Parasitology
Madison
Estados Unidos
World Association for the Advancement of Veterinary Parasitology - Materia
-
BIOLOGICAL CONTROL
DUDDINGTONIA FLAGRANS
GASTROINTESTINAL NEMATODES
CATTLE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/160726
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Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodesZegbi, SaraSagüés, María FedericaSaumell, Carlos AlfredoIglesias, Lucía EmiliaGuerrero, InesFernández, Alicia SilvinaBIOLOGICAL CONTROLDUDDINGTONIA FLAGRANSGASTROINTESTINAL NEMATODESCATTLEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes.Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Guerrero, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina27th Conference of the World Association for the Advancement of Veterinary ParasitologyMadisonEstados UnidosWorld Association for the Advancement of Veterinary ParasitologyWorld Association for the Advancement of Veterinary Parasitology (WAAVP)2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160726Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 257-257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.cominfo:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.com/wp-content/uploads/2019/07/WAAVP2019-Abstract-Book.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:10Zoai:ri.conicet.gov.ar:11336/160726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:11.211CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes |
title |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes |
spellingShingle |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes Zegbi, Sara BIOLOGICAL CONTROL DUDDINGTONIA FLAGRANS GASTROINTESTINAL NEMATODES CATTLE |
title_short |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes |
title_full |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes |
title_fullStr |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes |
title_full_unstemmed |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes |
title_sort |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes |
dc.creator.none.fl_str_mv |
Zegbi, Sara Sagüés, María Federica Saumell, Carlos Alfredo Iglesias, Lucía Emilia Guerrero, Ines Fernández, Alicia Silvina |
author |
Zegbi, Sara |
author_facet |
Zegbi, Sara Sagüés, María Federica Saumell, Carlos Alfredo Iglesias, Lucía Emilia Guerrero, Ines Fernández, Alicia Silvina |
author_role |
author |
author2 |
Sagüés, María Federica Saumell, Carlos Alfredo Iglesias, Lucía Emilia Guerrero, Ines Fernández, Alicia Silvina |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
BIOLOGICAL CONTROL DUDDINGTONIA FLAGRANS GASTROINTESTINAL NEMATODES CATTLE |
topic |
BIOLOGICAL CONTROL DUDDINGTONIA FLAGRANS GASTROINTESTINAL NEMATODES CATTLE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes. Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Guerrero, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina 27th Conference of the World Association for the Advancement of Veterinary Parasitology Madison Estados Unidos World Association for the Advancement of Veterinary Parasitology |
description |
The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Conferencia Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/160726 Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 257-257 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/160726 |
identifier_str_mv |
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 257-257 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.com info:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.com/wp-content/uploads/2019/07/WAAVP2019-Abstract-Book.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
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Nacional |
dc.publisher.none.fl_str_mv |
World Association for the Advancement of Veterinary Parasitology (WAAVP) |
publisher.none.fl_str_mv |
World Association for the Advancement of Veterinary Parasitology (WAAVP) |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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