Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes

Autores
Zegbi, Sara; Sagüés, María Federica; Saumell, Carlos Alfredo; Iglesias, Lucía Emilia; Guerrero, Ines; Fernández, Alicia Silvina
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes.
Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Guerrero, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
27th Conference of the World Association for the Advancement of Veterinary Parasitology
Madison
Estados Unidos
World Association for the Advancement of Veterinary Parasitology
Materia
BIOLOGICAL CONTROL
DUDDINGTONIA FLAGRANS
GASTROINTESTINAL NEMATODES
CATTLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160726
Acceder
id CONICETDig_ceb1bc4e2312b78a7a1aac4fa8327846
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodesZegbi, SaraSagüés, María FedericaSaumell, Carlos AlfredoIglesias, Lucía EmiliaGuerrero, InesFernández, Alicia SilvinaBIOLOGICAL CONTROLDUDDINGTONIA FLAGRANSGASTROINTESTINAL NEMATODESCATTLEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes.Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Guerrero, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina27th Conference of the World Association for the Advancement of Veterinary ParasitologyMadisonEstados UnidosWorld Association for the Advancement of Veterinary ParasitologyWorld Association for the Advancement of Veterinary Parasitology (WAAVP)2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160726Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 257-257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.cominfo:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.com/wp-content/uploads/2019/07/WAAVP2019-Abstract-Book.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:43:37Zoai:ri.conicet.gov.ar:11336/160726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:43:37.958CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
title Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
spellingShingle Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
Zegbi, Sara
BIOLOGICAL CONTROL
DUDDINGTONIA FLAGRANS
GASTROINTESTINAL NEMATODES
CATTLE
title_short Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
title_full Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
title_fullStr Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
title_full_unstemmed Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
title_sort Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes
dc.creator.none.fl_str_mv Zegbi, Sara
Sagüés, María Federica
Saumell, Carlos Alfredo
Iglesias, Lucía Emilia
Guerrero, Ines
Fernández, Alicia Silvina
author Zegbi, Sara
author_facet Zegbi, Sara
Sagüés, María Federica
Saumell, Carlos Alfredo
Iglesias, Lucía Emilia
Guerrero, Ines
Fernández, Alicia Silvina
author_role author
author2 Sagüés, María Federica
Saumell, Carlos Alfredo
Iglesias, Lucía Emilia
Guerrero, Ines
Fernández, Alicia Silvina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL CONTROL
DUDDINGTONIA FLAGRANS
GASTROINTESTINAL NEMATODES
CATTLE
topic BIOLOGICAL CONTROL
DUDDINGTONIA FLAGRANS
GASTROINTESTINAL NEMATODES
CATTLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes.
Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Guerrero, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
27th Conference of the World Association for the Advancement of Veterinary Parasitology
Madison
Estados Unidos
World Association for the Advancement of Veterinary Parasitology
description The nematophagous fungus Duddingtonia flagrans is a biocontrol agent that reduces the number of infective larvae of gastrointestinal nematodes in animal faeces, thus, lowering pasture infectivity. However, the minimum amount of fungal chlamydospores in faeces required for this effect is largely unknown. The following in vitro study aimed to determine the fungal efficacy of four different chlamydospores concentrations against three different levels of cattle faecal eggcounts. The chlamydospores concentrations tested were 11000, 6250, 3000 and 1000chl/g faeces. Faeces from naturally-infected calves were used to obtain faecal egg counts of 100, 480 and 840 epg. Ten faecal cultures of 10 g faeces each were set up for each chlamydospores concentration/faecal egg count combination, plus one control group for each faecal egg count level without chlamydospores. All cultures were kept at room temperature (20-26ºC) for two weeks and then L3 were recovered from each individual culture by overnight baermannisation, counted and identified. Thelarval reductions by D. flagrans, in decreasing order of chlamydospores concentrations, were: 100% (P<0.0001), 99% (P<0.0001),92% (P=0.0032) and 77% (ns) comparedto the 100 epg control; 100% (P<0.0001),98% (P<0.0001), 98% (P=0.0002) and 92%(ns) compared to the 480 epg control; and100% (P<0.0001), 100% (P<0.0001), 99%(P<0.0001) and 96% (ns) compared to the840 epg control. There were no differences between the three levels of faecal egg counts at any given fungal concentration, which indicates that the numbers of nematode eggsin cattle faeces would not be a determining factor for the efficacy of D. flagrans. These results suggest that the concentration of 1000 chl/g faeces would not be sufficient to achieve a significant fungal efficacy; however,it could be argued that, in practice, larval reductions of 77% to 96% would contribute tocontrol gastrointestinal nematodes.
publishDate 2019
dc.date.none.fl_str_mv 2019
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http://purl.org/coar/resource_type/c_5794
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160726
Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 257-257
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160726
identifier_str_mv Efficacy of different concentrations of duddingtonia flagrans chlamydospores against different levels of faecal egg counts of cattle gastrointestinal nematodes; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 257-257
CONICET Digital
CONICET
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dc.publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology (WAAVP)
publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology (WAAVP)
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