Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration

Autores
van Wijk, Ringo; Zhang, Qianqian; Zarza, Xavier; Lamers, Mart; Reyes Marquez, Francisca; Guardia, Aisha Elena; Scuffi, Denise; Garcia-Mata, Carlos; Ligterink, Wilco; Haring, Michel A.; Laxalt, Ana Maria; Munnik, Teun
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phospholipase C (PLC) has been suggested to play important roles in plant stress and development. To increase our understanding of PLC signaling in plants, we have started to analyze knock-out (KO), knock-down (KD) and overexpression mutants of Arabidopsis thaliana, which contains nine PLCs. Earlier, we characterized PLC2, PLC3 and PLC5. Here, the role of PLC7 is functionally addressed. Promoter-GUS analyses revealed that PLC7 is specifically expressed in the phloem of roots, leaves and flowers, and is also present in trichomes and hydathodes. Two T-DNA insertion mutants were obtained, i.e., plc7-3 being a KO- and plc7-4 a KD line. In contrast to earlier characterized phloem-expressed PLC mutants, i.e., plc3 and plc5, no defects in primary- or lateral root development were found for plc7 mutants. Like plc3 mutants, they were less sensitive to ABA during stomatal closure. Double-knockout plc3 plc7 lines were lethal, but plc5 plc7 (plc5/7) double mutants were viable, and revealed several new phenotypes, not observed earlier in the single mutants. These include a defect in seed mucilage, enhanced leaf serration, and an increased tolerance to drought. Overexpression of PLC7 enhanced drought tolerance too, similar to what was earlier found for PLC3-and PLC5 overexpression. In vivo 32Pi-labeling of seedlings and treatment with sorbitol to mimic drought stress, revealed stronger PIP2 responses in both drought-tolerant plc5/7 and PLC7-OE mutants. Together, these results show novel functions for PLC in plant stress and development. Potential molecular mechanisms are discussed.
Fil: van Wijk, Ringo. University of Amsterdam; Países Bajos
Fil: Zhang, Qianqian. University of Amsterdam; Países Bajos
Fil: Zarza, Xavier. University of Amsterdam; Países Bajos
Fil: Lamers, Mart. University of Amsterdam; Países Bajos
Fil: Reyes Marquez, Francisca. Wageningen University and Research, Wageningen; Países Bajos
Fil: Guardia, Aisha Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Scuffi, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Garcia-Mata, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Ligterink, Wilco. Wageningen University and Research, Wageningen; Países Bajos
Fil: Haring, Michel A.. University of Amsterdam; Países Bajos
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Munnik, Teun. University of Amsterdam; Países Bajos
Materia
PLC
SEED MUCILAGE
LEAF SERRATION
ABA SENSITIVITY
DROUGHT TOLERANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102883
Acceder
id CONICETDig_ce7092a74fbccfc1368894b3c4192b3a
oai_identifier_str oai:ri.conicet.gov.ar:11336/102883
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serrationvan Wijk, RingoZhang, QianqianZarza, XavierLamers, MartReyes Marquez, FranciscaGuardia, Aisha ElenaScuffi, DeniseGarcia-Mata, CarlosLigterink, WilcoHaring, Michel A.Laxalt, Ana MariaMunnik, TeunPLCSEED MUCILAGELEAF SERRATIONABA SENSITIVITYDROUGHT TOLERANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phospholipase C (PLC) has been suggested to play important roles in plant stress and development. To increase our understanding of PLC signaling in plants, we have started to analyze knock-out (KO), knock-down (KD) and overexpression mutants of Arabidopsis thaliana, which contains nine PLCs. Earlier, we characterized PLC2, PLC3 and PLC5. Here, the role of PLC7 is functionally addressed. Promoter-GUS analyses revealed that PLC7 is specifically expressed in the phloem of roots, leaves and flowers, and is also present in trichomes and hydathodes. Two T-DNA insertion mutants were obtained, i.e., plc7-3 being a KO- and plc7-4 a KD line. In contrast to earlier characterized phloem-expressed PLC mutants, i.e., plc3 and plc5, no defects in primary- or lateral root development were found for plc7 mutants. Like plc3 mutants, they were less sensitive to ABA during stomatal closure. Double-knockout plc3 plc7 lines were lethal, but plc5 plc7 (plc5/7) double mutants were viable, and revealed several new phenotypes, not observed earlier in the single mutants. These include a defect in seed mucilage, enhanced leaf serration, and an increased tolerance to drought. Overexpression of PLC7 enhanced drought tolerance too, similar to what was earlier found for PLC3-and PLC5 overexpression. In vivo 32Pi-labeling of seedlings and treatment with sorbitol to mimic drought stress, revealed stronger PIP2 responses in both drought-tolerant plc5/7 and PLC7-OE mutants. Together, these results show novel functions for PLC in plant stress and development. Potential molecular mechanisms are discussed.Fil: van Wijk, Ringo. University of Amsterdam; Países BajosFil: Zhang, Qianqian. University of Amsterdam; Países BajosFil: Zarza, Xavier. University of Amsterdam; Países BajosFil: Lamers, Mart. University of Amsterdam; Países BajosFil: Reyes Marquez, Francisca. Wageningen University and Research, Wageningen; Países BajosFil: Guardia, Aisha Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Scuffi, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Garcia-Mata, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Ligterink, Wilco. Wageningen University and Research, Wageningen; Países BajosFil: Haring, Michel A.. University of Amsterdam; Países BajosFil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Munnik, Teun. University of Amsterdam; Países BajosFrontiers Media S.A.2018-11-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102883van Wijk, Ringo; Zhang, Qianqian; Zarza, Xavier; Lamers, Mart; Reyes Marquez, Francisca; et al.; Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration; Frontiers Media S.A.; Frontiers in Plant Science; 9; 1721; 27-11-2018; 1-161664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fpls.2018.01721/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2018.01721info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:24Zoai:ri.conicet.gov.ar:11336/102883instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:24.599CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
title Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
spellingShingle Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
van Wijk, Ringo
PLC
SEED MUCILAGE
LEAF SERRATION
ABA SENSITIVITY
DROUGHT TOLERANCE
title_short Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
title_full Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
title_fullStr Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
title_full_unstemmed Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
title_sort Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration
dc.creator.none.fl_str_mv van Wijk, Ringo
Zhang, Qianqian
Zarza, Xavier
Lamers, Mart
Reyes Marquez, Francisca
Guardia, Aisha Elena
Scuffi, Denise
Garcia-Mata, Carlos
Ligterink, Wilco
Haring, Michel A.
Laxalt, Ana Maria
Munnik, Teun
author van Wijk, Ringo
author_facet van Wijk, Ringo
Zhang, Qianqian
Zarza, Xavier
Lamers, Mart
Reyes Marquez, Francisca
Guardia, Aisha Elena
Scuffi, Denise
Garcia-Mata, Carlos
Ligterink, Wilco
Haring, Michel A.
Laxalt, Ana Maria
Munnik, Teun
author_role author
author2 Zhang, Qianqian
Zarza, Xavier
Lamers, Mart
Reyes Marquez, Francisca
Guardia, Aisha Elena
Scuffi, Denise
Garcia-Mata, Carlos
Ligterink, Wilco
Haring, Michel A.
Laxalt, Ana Maria
Munnik, Teun
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLC
SEED MUCILAGE
LEAF SERRATION
ABA SENSITIVITY
DROUGHT TOLERANCE
topic PLC
SEED MUCILAGE
LEAF SERRATION
ABA SENSITIVITY
DROUGHT TOLERANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phospholipase C (PLC) has been suggested to play important roles in plant stress and development. To increase our understanding of PLC signaling in plants, we have started to analyze knock-out (KO), knock-down (KD) and overexpression mutants of Arabidopsis thaliana, which contains nine PLCs. Earlier, we characterized PLC2, PLC3 and PLC5. Here, the role of PLC7 is functionally addressed. Promoter-GUS analyses revealed that PLC7 is specifically expressed in the phloem of roots, leaves and flowers, and is also present in trichomes and hydathodes. Two T-DNA insertion mutants were obtained, i.e., plc7-3 being a KO- and plc7-4 a KD line. In contrast to earlier characterized phloem-expressed PLC mutants, i.e., plc3 and plc5, no defects in primary- or lateral root development were found for plc7 mutants. Like plc3 mutants, they were less sensitive to ABA during stomatal closure. Double-knockout plc3 plc7 lines were lethal, but plc5 plc7 (plc5/7) double mutants were viable, and revealed several new phenotypes, not observed earlier in the single mutants. These include a defect in seed mucilage, enhanced leaf serration, and an increased tolerance to drought. Overexpression of PLC7 enhanced drought tolerance too, similar to what was earlier found for PLC3-and PLC5 overexpression. In vivo 32Pi-labeling of seedlings and treatment with sorbitol to mimic drought stress, revealed stronger PIP2 responses in both drought-tolerant plc5/7 and PLC7-OE mutants. Together, these results show novel functions for PLC in plant stress and development. Potential molecular mechanisms are discussed.
Fil: van Wijk, Ringo. University of Amsterdam; Países Bajos
Fil: Zhang, Qianqian. University of Amsterdam; Países Bajos
Fil: Zarza, Xavier. University of Amsterdam; Países Bajos
Fil: Lamers, Mart. University of Amsterdam; Países Bajos
Fil: Reyes Marquez, Francisca. Wageningen University and Research, Wageningen; Países Bajos
Fil: Guardia, Aisha Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Scuffi, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Garcia-Mata, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Ligterink, Wilco. Wageningen University and Research, Wageningen; Países Bajos
Fil: Haring, Michel A.. University of Amsterdam; Países Bajos
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Munnik, Teun. University of Amsterdam; Países Bajos
description Phospholipase C (PLC) has been suggested to play important roles in plant stress and development. To increase our understanding of PLC signaling in plants, we have started to analyze knock-out (KO), knock-down (KD) and overexpression mutants of Arabidopsis thaliana, which contains nine PLCs. Earlier, we characterized PLC2, PLC3 and PLC5. Here, the role of PLC7 is functionally addressed. Promoter-GUS analyses revealed that PLC7 is specifically expressed in the phloem of roots, leaves and flowers, and is also present in trichomes and hydathodes. Two T-DNA insertion mutants were obtained, i.e., plc7-3 being a KO- and plc7-4 a KD line. In contrast to earlier characterized phloem-expressed PLC mutants, i.e., plc3 and plc5, no defects in primary- or lateral root development were found for plc7 mutants. Like plc3 mutants, they were less sensitive to ABA during stomatal closure. Double-knockout plc3 plc7 lines were lethal, but plc5 plc7 (plc5/7) double mutants were viable, and revealed several new phenotypes, not observed earlier in the single mutants. These include a defect in seed mucilage, enhanced leaf serration, and an increased tolerance to drought. Overexpression of PLC7 enhanced drought tolerance too, similar to what was earlier found for PLC3-and PLC5 overexpression. In vivo 32Pi-labeling of seedlings and treatment with sorbitol to mimic drought stress, revealed stronger PIP2 responses in both drought-tolerant plc5/7 and PLC7-OE mutants. Together, these results show novel functions for PLC in plant stress and development. Potential molecular mechanisms are discussed.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102883
van Wijk, Ringo; Zhang, Qianqian; Zarza, Xavier; Lamers, Mart; Reyes Marquez, Francisca; et al.; Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration; Frontiers Media S.A.; Frontiers in Plant Science; 9; 1721; 27-11-2018; 1-16
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102883
identifier_str_mv van Wijk, Ringo; Zhang, Qianqian; Zarza, Xavier; Lamers, Mart; Reyes Marquez, Francisca; et al.; Role for arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration; Frontiers Media S.A.; Frontiers in Plant Science; 9; 1721; 27-11-2018; 1-16
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fpls.2018.01721/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2018.01721
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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