Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats

Autores
Cataldi, Natalia Isabel; Lux, Victoria Adela R.; Libertun, Carlos
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Orexin A and orexin B (hypocretins) are neuropeptidessynthesized mainly by neurons located in the lateral hypothalamus andprojections throughout the brain. They are agonists at both the orexin 1and orexin 2 G protein-coupled receptors. They have been related toarousal, sleep and feeding, autonomic and neuroendocrine functions. Theirrole in the brain control of gonadotropins secretion was postulated inrodents and humans. Previously, we demonstrated the participation of theorexinergic system in attaining successful reproduction in in vivostudies. Methods: We studied in vitro the effects of both neuropeptides, in thepresence or absence of selective antagonists, on the mRNA expression oforexin 1 and orexin 2 receptors in anterior pituitary cells of proestrousrats, as well as the direct effects on FSH and LH secretion. Results: Both orexin A and orexin B increased FSH and LH secretion, theseeffects was suppressed by the orexin 1 receptor blocking agent SB-334867and the orexin 2 receptor antagonists JNJ-10397049. Orexin A and orexin Bdecreased of OX1 receptor mRNA expression and this effect was modifiedonly when both blocking agent were present. Neither orexin A nor theblocking drugs by themselves modified OX2 receptor mRNA expression.Orexin B treatment increased the mRNA expression of OX2 receptor. Theeffect was abolished only by the OX2 receptor antagonist. Conclusion: In an in vitro model, we demonstrated a direct effect oforexins on gonadotropins release and orexins receptors expression,underling the hypothesis that orexins participate in the brain control ofpituitary functions.
Fil: Cataldi, Natalia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Materia
Orexins receptors
Adenohypophysis
Antagonists
Gonadotropins
Hypocretins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102633
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102633
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous ratsCataldi, Natalia IsabelLux, Victoria Adela R.Libertun, CarlosOrexins receptorsAdenohypophysisAntagonistsGonadotropinsHypocretinshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Orexin A and orexin B (hypocretins) are neuropeptidessynthesized mainly by neurons located in the lateral hypothalamus andprojections throughout the brain. They are agonists at both the orexin 1and orexin 2 G protein-coupled receptors. They have been related toarousal, sleep and feeding, autonomic and neuroendocrine functions. Theirrole in the brain control of gonadotropins secretion was postulated inrodents and humans. Previously, we demonstrated the participation of theorexinergic system in attaining successful reproduction in in vivostudies. Methods: We studied in vitro the effects of both neuropeptides, in thepresence or absence of selective antagonists, on the mRNA expression oforexin 1 and orexin 2 receptors in anterior pituitary cells of proestrousrats, as well as the direct effects on FSH and LH secretion. Results: Both orexin A and orexin B increased FSH and LH secretion, theseeffects was suppressed by the orexin 1 receptor blocking agent SB-334867and the orexin 2 receptor antagonists JNJ-10397049. Orexin A and orexin Bdecreased of OX1 receptor mRNA expression and this effect was modifiedonly when both blocking agent were present. Neither orexin A nor theblocking drugs by themselves modified OX2 receptor mRNA expression.Orexin B treatment increased the mRNA expression of OX2 receptor. Theeffect was abolished only by the OX2 receptor antagonist. Conclusion: In an in vitro model, we demonstrated a direct effect oforexins on gonadotropins release and orexins receptors expression,underling the hypothesis that orexins participate in the brain control ofpituitary functions.Fil: Cataldi, Natalia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaElsevier Science2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102633Cataldi, Natalia Isabel; Lux, Victoria Adela R.; Libertun, Carlos; Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats; Elsevier Science; Regulatory Peptides; 188; 1-2014; 25-300167-0115CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0167011513001705info:eu-repo/semantics/altIdentifier/doi/10.1016/j.regpep.2013.12.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:41Zoai:ri.conicet.gov.ar:11336/102633instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:42.239CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
title Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
spellingShingle Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
Cataldi, Natalia Isabel
Orexins receptors
Adenohypophysis
Antagonists
Gonadotropins
Hypocretins
title_short Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
title_full Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
title_fullStr Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
title_full_unstemmed Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
title_sort Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats
dc.creator.none.fl_str_mv Cataldi, Natalia Isabel
Lux, Victoria Adela R.
Libertun, Carlos
author Cataldi, Natalia Isabel
author_facet Cataldi, Natalia Isabel
Lux, Victoria Adela R.
Libertun, Carlos
author_role author
author2 Lux, Victoria Adela R.
Libertun, Carlos
author2_role author
author
dc.subject.none.fl_str_mv Orexins receptors
Adenohypophysis
Antagonists
Gonadotropins
Hypocretins
topic Orexins receptors
Adenohypophysis
Antagonists
Gonadotropins
Hypocretins
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Orexin A and orexin B (hypocretins) are neuropeptidessynthesized mainly by neurons located in the lateral hypothalamus andprojections throughout the brain. They are agonists at both the orexin 1and orexin 2 G protein-coupled receptors. They have been related toarousal, sleep and feeding, autonomic and neuroendocrine functions. Theirrole in the brain control of gonadotropins secretion was postulated inrodents and humans. Previously, we demonstrated the participation of theorexinergic system in attaining successful reproduction in in vivostudies. Methods: We studied in vitro the effects of both neuropeptides, in thepresence or absence of selective antagonists, on the mRNA expression oforexin 1 and orexin 2 receptors in anterior pituitary cells of proestrousrats, as well as the direct effects on FSH and LH secretion. Results: Both orexin A and orexin B increased FSH and LH secretion, theseeffects was suppressed by the orexin 1 receptor blocking agent SB-334867and the orexin 2 receptor antagonists JNJ-10397049. Orexin A and orexin Bdecreased of OX1 receptor mRNA expression and this effect was modifiedonly when both blocking agent were present. Neither orexin A nor theblocking drugs by themselves modified OX2 receptor mRNA expression.Orexin B treatment increased the mRNA expression of OX2 receptor. Theeffect was abolished only by the OX2 receptor antagonist. Conclusion: In an in vitro model, we demonstrated a direct effect oforexins on gonadotropins release and orexins receptors expression,underling the hypothesis that orexins participate in the brain control ofpituitary functions.
Fil: Cataldi, Natalia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
description Orexin A and orexin B (hypocretins) are neuropeptidessynthesized mainly by neurons located in the lateral hypothalamus andprojections throughout the brain. They are agonists at both the orexin 1and orexin 2 G protein-coupled receptors. They have been related toarousal, sleep and feeding, autonomic and neuroendocrine functions. Theirrole in the brain control of gonadotropins secretion was postulated inrodents and humans. Previously, we demonstrated the participation of theorexinergic system in attaining successful reproduction in in vivostudies. Methods: We studied in vitro the effects of both neuropeptides, in thepresence or absence of selective antagonists, on the mRNA expression oforexin 1 and orexin 2 receptors in anterior pituitary cells of proestrousrats, as well as the direct effects on FSH and LH secretion. Results: Both orexin A and orexin B increased FSH and LH secretion, theseeffects was suppressed by the orexin 1 receptor blocking agent SB-334867and the orexin 2 receptor antagonists JNJ-10397049. Orexin A and orexin Bdecreased of OX1 receptor mRNA expression and this effect was modifiedonly when both blocking agent were present. Neither orexin A nor theblocking drugs by themselves modified OX2 receptor mRNA expression.Orexin B treatment increased the mRNA expression of OX2 receptor. Theeffect was abolished only by the OX2 receptor antagonist. Conclusion: In an in vitro model, we demonstrated a direct effect oforexins on gonadotropins release and orexins receptors expression,underling the hypothesis that orexins participate in the brain control ofpituitary functions.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102633
Cataldi, Natalia Isabel; Lux, Victoria Adela R.; Libertun, Carlos; Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats; Elsevier Science; Regulatory Peptides; 188; 1-2014; 25-30
0167-0115
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102633
identifier_str_mv Cataldi, Natalia Isabel; Lux, Victoria Adela R.; Libertun, Carlos; Orexin A and B in vitro modify orexins receptors expression and Gonadotropins secretion of anterior pituitary cells of proestrous rats; Elsevier Science; Regulatory Peptides; 188; 1-2014; 25-30
0167-0115
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0167011513001705
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.regpep.2013.12.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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