Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice

Autores
Rachid, Mirta Maria; Matar, Chantal; Duarte, Jairo; Perdigon, Gabriela del Valle
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies on a murine model have demonstrated that the administration of Lactobacillus helveticus and Lactobacillus casei inhibits the development of fibrosarcoma and colon carcinoma, respectively. The aim of this work was to study the beneficial effects of the consumption of milk fermented by L. helveticus on a murine model for mammary carcinoma. Female BALB/c mice were challenged by a single subcutaneous injection of tumoral cells (American Type Culture Collection 4T1) in the left mammary gland. Prior to tumour injection, mice were fed for two, five or seven consecutive days with fermented milk. The following factors were monitored for 2 months: rate of tumour development, histological studies, apoptosis, phagocytic index, peritoneal macrophages, determination of β-glucuronidase enzyme in peritoneal macrophages, determination of γ-interferon (INFγ) and tumour necrosis factor-α (TNF-α) in blood serum, determination of CD4+, CD8+, interleukin-6 (IL-6), IL-10, TNF-α and INFγ by immunoperoxidase, and measurement of β-glucuronidase activity in intestinal fluid. The administration of L. helveticus delayed the development of the tumour in all cases, a 2- or 7-day feeding period being most effective. This work demonstrates that milk fermented with L. helveticus decreases the growth rate of mammary tumours. The effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, implicated in oestrogen synthesis.
Fil: Rachid, Mirta Maria. Universite de Moncton; Canadá. Universidad Nacional de Tucumán; Argentina
Fil: Matar, Chantal. Universite de Moncton; Canadá
Fil: Duarte, Jairo. Universite de Moncton; Canadá
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
Materia
Breast Cancer
Fermented Milk
Proteolytic Strain
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36724
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in miceRachid, Mirta MariaMatar, ChantalDuarte, JairoPerdigon, Gabriela del ValleBreast CancerFermented MilkProteolytic Strainhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previous studies on a murine model have demonstrated that the administration of Lactobacillus helveticus and Lactobacillus casei inhibits the development of fibrosarcoma and colon carcinoma, respectively. The aim of this work was to study the beneficial effects of the consumption of milk fermented by L. helveticus on a murine model for mammary carcinoma. Female BALB/c mice were challenged by a single subcutaneous injection of tumoral cells (American Type Culture Collection 4T1) in the left mammary gland. Prior to tumour injection, mice were fed for two, five or seven consecutive days with fermented milk. The following factors were monitored for 2 months: rate of tumour development, histological studies, apoptosis, phagocytic index, peritoneal macrophages, determination of β-glucuronidase enzyme in peritoneal macrophages, determination of γ-interferon (INFγ) and tumour necrosis factor-α (TNF-α) in blood serum, determination of CD4+, CD8+, interleukin-6 (IL-6), IL-10, TNF-α and INFγ by immunoperoxidase, and measurement of β-glucuronidase activity in intestinal fluid. The administration of L. helveticus delayed the development of the tumour in all cases, a 2- or 7-day feeding period being most effective. This work demonstrates that milk fermented with L. helveticus decreases the growth rate of mammary tumours. The effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, implicated in oestrogen synthesis.Fil: Rachid, Mirta Maria. Universite de Moncton; Canadá. Universidad Nacional de Tucumán; ArgentinaFil: Matar, Chantal. Universite de Moncton; CanadáFil: Duarte, Jairo. Universite de Moncton; CanadáFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; ArgentinaWiley Blackwell Publishing, Inc2006-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36724Rachid, Mirta Maria; Matar, Chantal; Duarte, Jairo; Perdigon, Gabriela del Valle; Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice; Wiley Blackwell Publishing, Inc; Fems Immunology And Medical Microbiology; 47; 2; 7-2006; 242-2530928-8244CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695X.2006.00088.xinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/47/2/242/548958info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:36Zoai:ri.conicet.gov.ar:11336/36724instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:36.805CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
title Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
spellingShingle Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
Rachid, Mirta Maria
Breast Cancer
Fermented Milk
Proteolytic Strain
title_short Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
title_full Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
title_fullStr Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
title_full_unstemmed Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
title_sort Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
dc.creator.none.fl_str_mv Rachid, Mirta Maria
Matar, Chantal
Duarte, Jairo
Perdigon, Gabriela del Valle
author Rachid, Mirta Maria
author_facet Rachid, Mirta Maria
Matar, Chantal
Duarte, Jairo
Perdigon, Gabriela del Valle
author_role author
author2 Matar, Chantal
Duarte, Jairo
Perdigon, Gabriela del Valle
author2_role author
author
author
dc.subject.none.fl_str_mv Breast Cancer
Fermented Milk
Proteolytic Strain
topic Breast Cancer
Fermented Milk
Proteolytic Strain
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous studies on a murine model have demonstrated that the administration of Lactobacillus helveticus and Lactobacillus casei inhibits the development of fibrosarcoma and colon carcinoma, respectively. The aim of this work was to study the beneficial effects of the consumption of milk fermented by L. helveticus on a murine model for mammary carcinoma. Female BALB/c mice were challenged by a single subcutaneous injection of tumoral cells (American Type Culture Collection 4T1) in the left mammary gland. Prior to tumour injection, mice were fed for two, five or seven consecutive days with fermented milk. The following factors were monitored for 2 months: rate of tumour development, histological studies, apoptosis, phagocytic index, peritoneal macrophages, determination of β-glucuronidase enzyme in peritoneal macrophages, determination of γ-interferon (INFγ) and tumour necrosis factor-α (TNF-α) in blood serum, determination of CD4+, CD8+, interleukin-6 (IL-6), IL-10, TNF-α and INFγ by immunoperoxidase, and measurement of β-glucuronidase activity in intestinal fluid. The administration of L. helveticus delayed the development of the tumour in all cases, a 2- or 7-day feeding period being most effective. This work demonstrates that milk fermented with L. helveticus decreases the growth rate of mammary tumours. The effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, implicated in oestrogen synthesis.
Fil: Rachid, Mirta Maria. Universite de Moncton; Canadá. Universidad Nacional de Tucumán; Argentina
Fil: Matar, Chantal. Universite de Moncton; Canadá
Fil: Duarte, Jairo. Universite de Moncton; Canadá
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
description Previous studies on a murine model have demonstrated that the administration of Lactobacillus helveticus and Lactobacillus casei inhibits the development of fibrosarcoma and colon carcinoma, respectively. The aim of this work was to study the beneficial effects of the consumption of milk fermented by L. helveticus on a murine model for mammary carcinoma. Female BALB/c mice were challenged by a single subcutaneous injection of tumoral cells (American Type Culture Collection 4T1) in the left mammary gland. Prior to tumour injection, mice were fed for two, five or seven consecutive days with fermented milk. The following factors were monitored for 2 months: rate of tumour development, histological studies, apoptosis, phagocytic index, peritoneal macrophages, determination of β-glucuronidase enzyme in peritoneal macrophages, determination of γ-interferon (INFγ) and tumour necrosis factor-α (TNF-α) in blood serum, determination of CD4+, CD8+, interleukin-6 (IL-6), IL-10, TNF-α and INFγ by immunoperoxidase, and measurement of β-glucuronidase activity in intestinal fluid. The administration of L. helveticus delayed the development of the tumour in all cases, a 2- or 7-day feeding period being most effective. This work demonstrates that milk fermented with L. helveticus decreases the growth rate of mammary tumours. The effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, implicated in oestrogen synthesis.
publishDate 2006
dc.date.none.fl_str_mv 2006-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36724
Rachid, Mirta Maria; Matar, Chantal; Duarte, Jairo; Perdigon, Gabriela del Valle; Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice; Wiley Blackwell Publishing, Inc; Fems Immunology And Medical Microbiology; 47; 2; 7-2006; 242-253
0928-8244
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36724
identifier_str_mv Rachid, Mirta Maria; Matar, Chantal; Duarte, Jairo; Perdigon, Gabriela del Valle; Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice; Wiley Blackwell Publishing, Inc; Fems Immunology And Medical Microbiology; 47; 2; 7-2006; 242-253
0928-8244
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695X.2006.00088.x
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/47/2/242/548958
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268612970151936
score 13.13397

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