Proteolytic activity of Lactobacillus strains on soybean proteins

Autores
Aguirre, Laura Beatriz María; Hebert, Elvira Maria; Garro, Marisa Selva; Savoy, Graciela
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The capacity of Lactobacillus strains for degrading soy proteins was evaluated. The hydrolytic action was monitored by SDSePAGE and reverse phase-HPLC analyses. Peptidase activities were also determined using synthetic chromogenic substrates. All assayed strains were able to hydrolyze a'-subunit of bconglycinin while for the other fractions the degree of degradation was strain-dependent. The pattern of peptides produced from soy proteins hydrolysis differed amongst the lactobacilli studied, suggesting different proteolytic enzyme specificities.
Fil: Aguirre, Laura Beatriz María. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento Biomedico. Cátedra de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Lactobacillus
Proteolytic
Enzymes
Soy Protein Hydrolysis
Bacterias Lacticas
Proteolisis
Enzimas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20205
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20205
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteolytic activity of Lactobacillus strains on soybean proteinsAguirre, Laura Beatriz MaríaHebert, Elvira MariaGarro, Marisa SelvaSavoy, GracielaLactobacillusProteolyticEnzymesSoy Protein HydrolysisBacterias LacticasProteolisisEnzimashttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The capacity of Lactobacillus strains for degrading soy proteins was evaluated. The hydrolytic action was monitored by SDSePAGE and reverse phase-HPLC analyses. Peptidase activities were also determined using synthetic chromogenic substrates. All assayed strains were able to hydrolyze a'-subunit of bconglycinin while for the other fractions the degree of degradation was strain-dependent. The pattern of peptides produced from soy proteins hydrolysis differed amongst the lactobacilli studied, suggesting different proteolytic enzyme specificities.Fil: Aguirre, Laura Beatriz María. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento Biomedico. Cátedra de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier Science2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20205Aguirre, Laura Beatriz María; Hebert, Elvira Maria; Garro, Marisa Selva; Savoy, Graciela; Proteolytic activity of Lactobacillus strains on soybean proteins; Elsevier Science; LWT - Food Science and Technology; 59; 2 Parte 1; 7-2014; 780-7850023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2014.06.061info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643814004290?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:09:27Zoai:ri.conicet.gov.ar:11336/20205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:09:27.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteolytic activity of Lactobacillus strains on soybean proteins
title Proteolytic activity of Lactobacillus strains on soybean proteins
spellingShingle Proteolytic activity of Lactobacillus strains on soybean proteins
Aguirre, Laura Beatriz María
Lactobacillus
Proteolytic
Enzymes
Soy Protein Hydrolysis
Bacterias Lacticas
Proteolisis
Enzimas
title_short Proteolytic activity of Lactobacillus strains on soybean proteins
title_full Proteolytic activity of Lactobacillus strains on soybean proteins
title_fullStr Proteolytic activity of Lactobacillus strains on soybean proteins
title_full_unstemmed Proteolytic activity of Lactobacillus strains on soybean proteins
title_sort Proteolytic activity of Lactobacillus strains on soybean proteins
dc.creator.none.fl_str_mv Aguirre, Laura Beatriz María
Hebert, Elvira Maria
Garro, Marisa Selva
Savoy, Graciela
author Aguirre, Laura Beatriz María
author_facet Aguirre, Laura Beatriz María
Hebert, Elvira Maria
Garro, Marisa Selva
Savoy, Graciela
author_role author
author2 Hebert, Elvira Maria
Garro, Marisa Selva
Savoy, Graciela
author2_role author
author
author
dc.subject.none.fl_str_mv Lactobacillus
Proteolytic
Enzymes
Soy Protein Hydrolysis
Bacterias Lacticas
Proteolisis
Enzimas
topic Lactobacillus
Proteolytic
Enzymes
Soy Protein Hydrolysis
Bacterias Lacticas
Proteolisis
Enzimas
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The capacity of Lactobacillus strains for degrading soy proteins was evaluated. The hydrolytic action was monitored by SDSePAGE and reverse phase-HPLC analyses. Peptidase activities were also determined using synthetic chromogenic substrates. All assayed strains were able to hydrolyze a'-subunit of bconglycinin while for the other fractions the degree of degradation was strain-dependent. The pattern of peptides produced from soy proteins hydrolysis differed amongst the lactobacilli studied, suggesting different proteolytic enzyme specificities.
Fil: Aguirre, Laura Beatriz María. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento Biomedico. Cátedra de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description The capacity of Lactobacillus strains for degrading soy proteins was evaluated. The hydrolytic action was monitored by SDSePAGE and reverse phase-HPLC analyses. Peptidase activities were also determined using synthetic chromogenic substrates. All assayed strains were able to hydrolyze a'-subunit of bconglycinin while for the other fractions the degree of degradation was strain-dependent. The pattern of peptides produced from soy proteins hydrolysis differed amongst the lactobacilli studied, suggesting different proteolytic enzyme specificities.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20205
Aguirre, Laura Beatriz María; Hebert, Elvira Maria; Garro, Marisa Selva; Savoy, Graciela; Proteolytic activity of Lactobacillus strains on soybean proteins; Elsevier Science; LWT - Food Science and Technology; 59; 2 Parte 1; 7-2014; 780-785
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20205
identifier_str_mv Aguirre, Laura Beatriz María; Hebert, Elvira Maria; Garro, Marisa Selva; Savoy, Graciela; Proteolytic activity of Lactobacillus strains on soybean proteins; Elsevier Science; LWT - Food Science and Technology; 59; 2 Parte 1; 7-2014; 780-785
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2014.06.061
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643814004290?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847977608852013056
score 13.087074

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