Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)

Autores
Citores, Lucía; Ragucci, Sara; Russo, Rosita; Gay, Claudia Carolina; Chambery, Angela; Di Maro, Antimo; Iglesias, Rosario; Ferreras, José M.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have purified ledodin, a cytotoxic 22-kDa protein from shiitake mushroom (Lentinula edodes) consisting of a 197 amino acid chain. Ledodin possessed N-glycosylase activity on the sarcin-ricin loop of mammalian 28S rRNA and inhibited protein synthesis. However, it was not active against insect, fungal, and bacterial ribosomes. In vitro and in silico studies suggested that ledodin exhibits a catalytic mechanism like that of DNA glycosylases and plant ribosome-inactivating proteins. Moreover, the sequence and structure of ledodin was not related to any protein of known function, although ledodin-homologous sequences were found in the genome of several species of fungi, some edible, belonging to different orders of the class Agaricomycetes. Therefore, ledodin could be the first of a new family of enzymes widely distributed among this class of basidiomycetes. The interest of these proteins lies both, in the fact that they can be a toxic agent of some edible mushrooms and in their application in medicine and biotechnology.
Fil: Citores, Lucía. Universidad de Valladolid. Facultad de Ciencias; España
Fil: Ragucci, Sara. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Russo, Rosita. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Gay, Claudia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Chambery, Angela. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Di Maro, Antimo. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Iglesias, Rosario. Universidad de Valladolid; España
Fil: Ferreras, José M.. Universidad de Valladolid; España
Materia
PROTEIN SYNTHESIS (INHIBITION)
RIBOSOME-INACTIVATING PROTEIN (RIP)
RIBOTOXIN
RRNA N-GLYCOSYLASE
SHIITAKE (LENTINULA EDODES)
TOXIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212238
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212238
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)Citores, LucíaRagucci, SaraRusso, RositaGay, Claudia CarolinaChambery, AngelaDi Maro, AntimoIglesias, RosarioFerreras, José M.PROTEIN SYNTHESIS (INHIBITION)RIBOSOME-INACTIVATING PROTEIN (RIP)RIBOTOXINRRNA N-GLYCOSYLASESHIITAKE (LENTINULA EDODES)TOXINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have purified ledodin, a cytotoxic 22-kDa protein from shiitake mushroom (Lentinula edodes) consisting of a 197 amino acid chain. Ledodin possessed N-glycosylase activity on the sarcin-ricin loop of mammalian 28S rRNA and inhibited protein synthesis. However, it was not active against insect, fungal, and bacterial ribosomes. In vitro and in silico studies suggested that ledodin exhibits a catalytic mechanism like that of DNA glycosylases and plant ribosome-inactivating proteins. Moreover, the sequence and structure of ledodin was not related to any protein of known function, although ledodin-homologous sequences were found in the genome of several species of fungi, some edible, belonging to different orders of the class Agaricomycetes. Therefore, ledodin could be the first of a new family of enzymes widely distributed among this class of basidiomycetes. The interest of these proteins lies both, in the fact that they can be a toxic agent of some edible mushrooms and in their application in medicine and biotechnology.Fil: Citores, Lucía. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Ragucci, Sara. Seconda Universita Degli Studi Di Napoli; ItaliaFil: Russo, Rosita. Seconda Universita Degli Studi Di Napoli; ItaliaFil: Gay, Claudia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Chambery, Angela. Seconda Universita Degli Studi Di Napoli; ItaliaFil: Di Maro, Antimo. Seconda Universita Degli Studi Di Napoli; ItaliaFil: Iglesias, Rosario. Universidad de Valladolid; EspañaFil: Ferreras, José M.. Universidad de Valladolid; EspañaJohn Wiley & Sons2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212238Citores, Lucía; Ragucci, Sara; Russo, Rosita; Gay, Claudia Carolina; Chambery, Angela; et al.; Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes); John Wiley & Sons; Protein Science; 32; 4; 3-2023; 1-170961-8368CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/pro.4621info:eu-repo/semantics/altIdentifier/doi/10.1002/pro.4621info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:05Zoai:ri.conicet.gov.ar:11336/212238instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:05.918CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
title Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
spellingShingle Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
Citores, Lucía
PROTEIN SYNTHESIS (INHIBITION)
RIBOSOME-INACTIVATING PROTEIN (RIP)
RIBOTOXIN
RRNA N-GLYCOSYLASE
SHIITAKE (LENTINULA EDODES)
TOXIN
title_short Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
title_full Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
title_fullStr Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
title_full_unstemmed Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
title_sort Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes)
dc.creator.none.fl_str_mv Citores, Lucía
Ragucci, Sara
Russo, Rosita
Gay, Claudia Carolina
Chambery, Angela
Di Maro, Antimo
Iglesias, Rosario
Ferreras, José M.
author Citores, Lucía
author_facet Citores, Lucía
Ragucci, Sara
Russo, Rosita
Gay, Claudia Carolina
Chambery, Angela
Di Maro, Antimo
Iglesias, Rosario
Ferreras, José M.
author_role author
author2 Ragucci, Sara
Russo, Rosita
Gay, Claudia Carolina
Chambery, Angela
Di Maro, Antimo
Iglesias, Rosario
Ferreras, José M.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PROTEIN SYNTHESIS (INHIBITION)
RIBOSOME-INACTIVATING PROTEIN (RIP)
RIBOTOXIN
RRNA N-GLYCOSYLASE
SHIITAKE (LENTINULA EDODES)
TOXIN
topic PROTEIN SYNTHESIS (INHIBITION)
RIBOSOME-INACTIVATING PROTEIN (RIP)
RIBOTOXIN
RRNA N-GLYCOSYLASE
SHIITAKE (LENTINULA EDODES)
TOXIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have purified ledodin, a cytotoxic 22-kDa protein from shiitake mushroom (Lentinula edodes) consisting of a 197 amino acid chain. Ledodin possessed N-glycosylase activity on the sarcin-ricin loop of mammalian 28S rRNA and inhibited protein synthesis. However, it was not active against insect, fungal, and bacterial ribosomes. In vitro and in silico studies suggested that ledodin exhibits a catalytic mechanism like that of DNA glycosylases and plant ribosome-inactivating proteins. Moreover, the sequence and structure of ledodin was not related to any protein of known function, although ledodin-homologous sequences were found in the genome of several species of fungi, some edible, belonging to different orders of the class Agaricomycetes. Therefore, ledodin could be the first of a new family of enzymes widely distributed among this class of basidiomycetes. The interest of these proteins lies both, in the fact that they can be a toxic agent of some edible mushrooms and in their application in medicine and biotechnology.
Fil: Citores, Lucía. Universidad de Valladolid. Facultad de Ciencias; España
Fil: Ragucci, Sara. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Russo, Rosita. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Gay, Claudia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Chambery, Angela. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Di Maro, Antimo. Seconda Universita Degli Studi Di Napoli; Italia
Fil: Iglesias, Rosario. Universidad de Valladolid; España
Fil: Ferreras, José M.. Universidad de Valladolid; España
description We have purified ledodin, a cytotoxic 22-kDa protein from shiitake mushroom (Lentinula edodes) consisting of a 197 amino acid chain. Ledodin possessed N-glycosylase activity on the sarcin-ricin loop of mammalian 28S rRNA and inhibited protein synthesis. However, it was not active against insect, fungal, and bacterial ribosomes. In vitro and in silico studies suggested that ledodin exhibits a catalytic mechanism like that of DNA glycosylases and plant ribosome-inactivating proteins. Moreover, the sequence and structure of ledodin was not related to any protein of known function, although ledodin-homologous sequences were found in the genome of several species of fungi, some edible, belonging to different orders of the class Agaricomycetes. Therefore, ledodin could be the first of a new family of enzymes widely distributed among this class of basidiomycetes. The interest of these proteins lies both, in the fact that they can be a toxic agent of some edible mushrooms and in their application in medicine and biotechnology.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212238
Citores, Lucía; Ragucci, Sara; Russo, Rosita; Gay, Claudia Carolina; Chambery, Angela; et al.; Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes); John Wiley & Sons; Protein Science; 32; 4; 3-2023; 1-17
0961-8368
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212238
identifier_str_mv Citores, Lucía; Ragucci, Sara; Russo, Rosita; Gay, Claudia Carolina; Chambery, Angela; et al.; Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes); John Wiley & Sons; Protein Science; 32; 4; 3-2023; 1-17
0961-8368
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/pro.4621
info:eu-repo/semantics/altIdentifier/doi/10.1002/pro.4621
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270030974156800
score 13.13397

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