Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen

Autores
Salva, Maria Susana; Merino, Maria Cecilia; Villena, Julio Cesar; Gruppi, Adriana; Alvarez, Gladis Susana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Protein malnutrition decreases resistance to infection due to the alteration of various physiological processes, including hematopoiesis. This work evaluated the effect of protein malnutrition on the ontogeny and function of spleen and bone marrow (BM) B lymphocytes (LB). Weaning mice were malnourished by protein deficiency for 21d (M). Control mice (C) received balanced conventional diet. In M and C groups it was evaluated: expression of B220, HSA and IgM in BM and spleen cells by flow cytometry, total IgM and IgG levels in culture supernatants of spleen LB, stimulated with LPS or CpG, and proliferative capacity of spleen LB. Malnutrition decreased significantly the total cell number of BM and spleen, mainly affecting the population of LB (B220+). In BM, the M group showed a marked decrease in immature LB population (B220lowHSAhighIgM+/-) (p<0.01), whereas the spleen showed a decrease of mature LB (B220high HSAlowIgM+) (p<0.05), with respect to the C group. Levels of total IgM and IgG in the M group were not different from the C mice. LB proliferative capacity in response to LPS and CpG was similar in both groups. Malnutrition affects B lymphopoiesis in spleen and BM, decreasing production and number of LB, without affecting the functionality of these cells. According to these results, the increased susceptibility to infections in malnourished mice should be, at least in part, due to quantitative rather than qualitative alterations of LB.
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Merino, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gruppi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XXVI Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
Materia
Desnutrición
Linfocitos B
Bazo
Médula ósea
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277575
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/277575
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protein malnutrition affects the development of b lymphocytes in bone marrow and spleenSalva, Maria SusanaMerino, Maria CeciliaVillena, Julio CesarGruppi, AdrianaAlvarez, Gladis SusanaDesnutriciónLinfocitos BBazoMédula óseahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Protein malnutrition decreases resistance to infection due to the alteration of various physiological processes, including hematopoiesis. This work evaluated the effect of protein malnutrition on the ontogeny and function of spleen and bone marrow (BM) B lymphocytes (LB). Weaning mice were malnourished by protein deficiency for 21d (M). Control mice (C) received balanced conventional diet. In M and C groups it was evaluated: expression of B220, HSA and IgM in BM and spleen cells by flow cytometry, total IgM and IgG levels in culture supernatants of spleen LB, stimulated with LPS or CpG, and proliferative capacity of spleen LB. Malnutrition decreased significantly the total cell number of BM and spleen, mainly affecting the population of LB (B220+). In BM, the M group showed a marked decrease in immature LB population (B220lowHSAhighIgM+/-) (p<0.01), whereas the spleen showed a decrease of mature LB (B220high HSAlowIgM+) (p<0.05), with respect to the C group. Levels of total IgM and IgG in the M group were not different from the C mice. LB proliferative capacity in response to LPS and CpG was similar in both groups. Malnutrition affects B lymphopoiesis in spleen and BM, decreasing production and number of LB, without affecting the functionality of these cells. According to these results, the increased susceptibility to infections in malnourished mice should be, at least in part, due to quantitative rather than qualitative alterations of LB.Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Merino, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Gruppi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaXXVI Jornadas Científicas de la Asociación de Biología de TucumánTafí del ValleArgentinaAsociación de Biología de TucumánTech Science Press2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277575Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2009; 151-1510327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/listado.php?id=2Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:15:14Zoai:ri.conicet.gov.ar:11336/277575instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:15:14.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
title Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
spellingShingle Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
Salva, Maria Susana
Desnutrición
Linfocitos B
Bazo
Médula ósea
title_short Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
title_full Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
title_fullStr Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
title_full_unstemmed Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
title_sort Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen
dc.creator.none.fl_str_mv Salva, Maria Susana
Merino, Maria Cecilia
Villena, Julio Cesar
Gruppi, Adriana
Alvarez, Gladis Susana
author Salva, Maria Susana
author_facet Salva, Maria Susana
Merino, Maria Cecilia
Villena, Julio Cesar
Gruppi, Adriana
Alvarez, Gladis Susana
author_role author
author2 Merino, Maria Cecilia
Villena, Julio Cesar
Gruppi, Adriana
Alvarez, Gladis Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Desnutrición
Linfocitos B
Bazo
Médula ósea
topic Desnutrición
Linfocitos B
Bazo
Médula ósea
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Protein malnutrition decreases resistance to infection due to the alteration of various physiological processes, including hematopoiesis. This work evaluated the effect of protein malnutrition on the ontogeny and function of spleen and bone marrow (BM) B lymphocytes (LB). Weaning mice were malnourished by protein deficiency for 21d (M). Control mice (C) received balanced conventional diet. In M and C groups it was evaluated: expression of B220, HSA and IgM in BM and spleen cells by flow cytometry, total IgM and IgG levels in culture supernatants of spleen LB, stimulated with LPS or CpG, and proliferative capacity of spleen LB. Malnutrition decreased significantly the total cell number of BM and spleen, mainly affecting the population of LB (B220+). In BM, the M group showed a marked decrease in immature LB population (B220lowHSAhighIgM+/-) (p<0.01), whereas the spleen showed a decrease of mature LB (B220high HSAlowIgM+) (p<0.05), with respect to the C group. Levels of total IgM and IgG in the M group were not different from the C mice. LB proliferative capacity in response to LPS and CpG was similar in both groups. Malnutrition affects B lymphopoiesis in spleen and BM, decreasing production and number of LB, without affecting the functionality of these cells. According to these results, the increased susceptibility to infections in malnourished mice should be, at least in part, due to quantitative rather than qualitative alterations of LB.
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Merino, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gruppi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XXVI Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
description Protein malnutrition decreases resistance to infection due to the alteration of various physiological processes, including hematopoiesis. This work evaluated the effect of protein malnutrition on the ontogeny and function of spleen and bone marrow (BM) B lymphocytes (LB). Weaning mice were malnourished by protein deficiency for 21d (M). Control mice (C) received balanced conventional diet. In M and C groups it was evaluated: expression of B220, HSA and IgM in BM and spleen cells by flow cytometry, total IgM and IgG levels in culture supernatants of spleen LB, stimulated with LPS or CpG, and proliferative capacity of spleen LB. Malnutrition decreased significantly the total cell number of BM and spleen, mainly affecting the population of LB (B220+). In BM, the M group showed a marked decrease in immature LB population (B220lowHSAhighIgM+/-) (p<0.01), whereas the spleen showed a decrease of mature LB (B220high HSAlowIgM+) (p<0.05), with respect to the C group. Levels of total IgM and IgG in the M group were not different from the C mice. LB proliferative capacity in response to LPS and CpG was similar in both groups. Malnutrition affects B lymphopoiesis in spleen and BM, decreasing production and number of LB, without affecting the functionality of these cells. According to these results, the increased susceptibility to infections in malnourished mice should be, at least in part, due to quantitative rather than qualitative alterations of LB.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277575
Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2009; 151-151
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277575
identifier_str_mv Protein malnutrition affects the development of b lymphocytes in bone marrow and spleen; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2009; 151-151
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/listado.php?id=2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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