In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive

Autores
Gimeno, F. Luengo; Gatto, S. C.; Croxatto, Juan Oscar; Ferro, J. I.; Gallo, Juan Eduardo Maria
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose The use of standard sutures has been replaced by platelet-rich plasma (PRP), a bioadhesive agent, in several surgical procedures. This prompted us to test PRP efficacy in experimental lamellar keratoplasty. Methods After lamellar anterior keratoplasty, PRP with a mean concentration of 807 564 platelets/mm 3 was used to attach the corneal flap to the stromal surface in 12 New Zealand white rabbits. 10-0 nylon sutures were used in one control group of 12 animals and no suture was used in a second control group of six rabbits. Animals were killed at days 2, 7, 30, and 90 for histological and smooth muscle actin (SMA) immunohistochemical analysis. Results The PRP group showed a tight corneal graft from the first postoperative hours until they were killed. A transparent cornea was seen at 30 days and remained clear until the end of the protocol. Histological specimens showed no signs of ocular inflammation in any animal within the PRP group. Electron microscopy showed normal morphological features on the flap and stromal bed, and a clear interface zone without cells or debris. The number of stromal myofibroblasts was lower than that seen in the suture group at 3 months postsurgery. The sutured group showed an attached cornea with signs of inflammation around the knots. All flaps without PRP or sutures were completely detached in the immediate postoperative period. Conclusions PRP was useful for attaching the corneal flap and it was well tolerated by the rabbit corneal tissue. Corneal healing was satisfactory. Further studies on PRP adhesiveness in grafts with donor corneas should be performed before considering its use in patients.
Fil: Gimeno, F. Luengo. Universidad Austral; Argentina
Fil: Gatto, S. C.. Hospital Universitario Austral; Argentina
Fil: Croxatto, Juan Oscar. Fundación Oftalmología Argentina "J. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferro, J. I.. Hospital Universitario Austral; Argentina
Fil: Gallo, Juan Eduardo Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina
Materia
CORNEAL TRANSPLANTATION
LAMELLAR KERATOPLASTY
PLATELET-RICH-PLASMA
SUTURELESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96253
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesiveGimeno, F. LuengoGatto, S. C.Croxatto, Juan OscarFerro, J. I.Gallo, Juan Eduardo MariaCORNEAL TRANSPLANTATIONLAMELLAR KERATOPLASTYPLATELET-RICH-PLASMASUTURELESShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Purpose The use of standard sutures has been replaced by platelet-rich plasma (PRP), a bioadhesive agent, in several surgical procedures. This prompted us to test PRP efficacy in experimental lamellar keratoplasty. Methods After lamellar anterior keratoplasty, PRP with a mean concentration of 807 564 platelets/mm 3 was used to attach the corneal flap to the stromal surface in 12 New Zealand white rabbits. 10-0 nylon sutures were used in one control group of 12 animals and no suture was used in a second control group of six rabbits. Animals were killed at days 2, 7, 30, and 90 for histological and smooth muscle actin (SMA) immunohistochemical analysis. Results The PRP group showed a tight corneal graft from the first postoperative hours until they were killed. A transparent cornea was seen at 30 days and remained clear until the end of the protocol. Histological specimens showed no signs of ocular inflammation in any animal within the PRP group. Electron microscopy showed normal morphological features on the flap and stromal bed, and a clear interface zone without cells or debris. The number of stromal myofibroblasts was lower than that seen in the suture group at 3 months postsurgery. The sutured group showed an attached cornea with signs of inflammation around the knots. All flaps without PRP or sutures were completely detached in the immediate postoperative period. Conclusions PRP was useful for attaching the corneal flap and it was well tolerated by the rabbit corneal tissue. Corneal healing was satisfactory. Further studies on PRP adhesiveness in grafts with donor corneas should be performed before considering its use in patients.Fil: Gimeno, F. Luengo. Universidad Austral; ArgentinaFil: Gatto, S. C.. Hospital Universitario Austral; ArgentinaFil: Croxatto, Juan Oscar. Fundación Oftalmología Argentina "J. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferro, J. I.. Hospital Universitario Austral; ArgentinaFil: Gallo, Juan Eduardo Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaNature Publishing Group2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96253Gimeno, F. Luengo; Gatto, S. C.; Croxatto, Juan Oscar; Ferro, J. I.; Gallo, Juan Eduardo Maria; In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive; Nature Publishing Group; Eye; 24; 2; 2-2010; 368-3750950-222XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/eye.2009.49info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/eye200949info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:01Zoai:ri.conicet.gov.ar:11336/96253instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:01.883CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
title In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
spellingShingle In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
Gimeno, F. Luengo
CORNEAL TRANSPLANTATION
LAMELLAR KERATOPLASTY
PLATELET-RICH-PLASMA
SUTURELESS
title_short In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
title_full In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
title_fullStr In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
title_full_unstemmed In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
title_sort In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive
dc.creator.none.fl_str_mv Gimeno, F. Luengo
Gatto, S. C.
Croxatto, Juan Oscar
Ferro, J. I.
Gallo, Juan Eduardo Maria
author Gimeno, F. Luengo
author_facet Gimeno, F. Luengo
Gatto, S. C.
Croxatto, Juan Oscar
Ferro, J. I.
Gallo, Juan Eduardo Maria
author_role author
author2 Gatto, S. C.
Croxatto, Juan Oscar
Ferro, J. I.
Gallo, Juan Eduardo Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv CORNEAL TRANSPLANTATION
LAMELLAR KERATOPLASTY
PLATELET-RICH-PLASMA
SUTURELESS
topic CORNEAL TRANSPLANTATION
LAMELLAR KERATOPLASTY
PLATELET-RICH-PLASMA
SUTURELESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose The use of standard sutures has been replaced by platelet-rich plasma (PRP), a bioadhesive agent, in several surgical procedures. This prompted us to test PRP efficacy in experimental lamellar keratoplasty. Methods After lamellar anterior keratoplasty, PRP with a mean concentration of 807 564 platelets/mm 3 was used to attach the corneal flap to the stromal surface in 12 New Zealand white rabbits. 10-0 nylon sutures were used in one control group of 12 animals and no suture was used in a second control group of six rabbits. Animals were killed at days 2, 7, 30, and 90 for histological and smooth muscle actin (SMA) immunohistochemical analysis. Results The PRP group showed a tight corneal graft from the first postoperative hours until they were killed. A transparent cornea was seen at 30 days and remained clear until the end of the protocol. Histological specimens showed no signs of ocular inflammation in any animal within the PRP group. Electron microscopy showed normal morphological features on the flap and stromal bed, and a clear interface zone without cells or debris. The number of stromal myofibroblasts was lower than that seen in the suture group at 3 months postsurgery. The sutured group showed an attached cornea with signs of inflammation around the knots. All flaps without PRP or sutures were completely detached in the immediate postoperative period. Conclusions PRP was useful for attaching the corneal flap and it was well tolerated by the rabbit corneal tissue. Corneal healing was satisfactory. Further studies on PRP adhesiveness in grafts with donor corneas should be performed before considering its use in patients.
Fil: Gimeno, F. Luengo. Universidad Austral; Argentina
Fil: Gatto, S. C.. Hospital Universitario Austral; Argentina
Fil: Croxatto, Juan Oscar. Fundación Oftalmología Argentina "J. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferro, J. I.. Hospital Universitario Austral; Argentina
Fil: Gallo, Juan Eduardo Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina
description Purpose The use of standard sutures has been replaced by platelet-rich plasma (PRP), a bioadhesive agent, in several surgical procedures. This prompted us to test PRP efficacy in experimental lamellar keratoplasty. Methods After lamellar anterior keratoplasty, PRP with a mean concentration of 807 564 platelets/mm 3 was used to attach the corneal flap to the stromal surface in 12 New Zealand white rabbits. 10-0 nylon sutures were used in one control group of 12 animals and no suture was used in a second control group of six rabbits. Animals were killed at days 2, 7, 30, and 90 for histological and smooth muscle actin (SMA) immunohistochemical analysis. Results The PRP group showed a tight corneal graft from the first postoperative hours until they were killed. A transparent cornea was seen at 30 days and remained clear until the end of the protocol. Histological specimens showed no signs of ocular inflammation in any animal within the PRP group. Electron microscopy showed normal morphological features on the flap and stromal bed, and a clear interface zone without cells or debris. The number of stromal myofibroblasts was lower than that seen in the suture group at 3 months postsurgery. The sutured group showed an attached cornea with signs of inflammation around the knots. All flaps without PRP or sutures were completely detached in the immediate postoperative period. Conclusions PRP was useful for attaching the corneal flap and it was well tolerated by the rabbit corneal tissue. Corneal healing was satisfactory. Further studies on PRP adhesiveness in grafts with donor corneas should be performed before considering its use in patients.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96253
Gimeno, F. Luengo; Gatto, S. C.; Croxatto, Juan Oscar; Ferro, J. I.; Gallo, Juan Eduardo Maria; In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive; Nature Publishing Group; Eye; 24; 2; 2-2010; 368-375
0950-222X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96253
identifier_str_mv Gimeno, F. Luengo; Gatto, S. C.; Croxatto, Juan Oscar; Ferro, J. I.; Gallo, Juan Eduardo Maria; In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive; Nature Publishing Group; Eye; 24; 2; 2-2010; 368-375
0950-222X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/eye.2009.49
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/eye200949
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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