Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro

Autores
López, Sofía; Andreoli, Valentina; Pereyra, Susana Beatriz; Romeo, Florencia; Delgado, Santiago Germán; Grolli, Stefano; Perez, Sandra; Alvarez, Ignacio; Verna, Andrea Elizabeth
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Diseases of the reproductive tract are a frequent problem in dairy herds. Herpesviruses cause several syndromes including uterine diseases. The role of bovine gammaherpesvirus 4 (BoGHV-4) in the development of endometritis has not been clearly described. Platelet-rich plasma (PRP) is an emerging therapeutic in tissue regeneration due to its enrichment in growth factors with mitogenic and anti-inflammatory potential. This study analysed the replication kinetics of BoGHV-4 in the presence of 5% and 10% PRP, compared to 10% fetal bovine serum. For this, Madin-Darby Bovine Kidney (MDBK) and primary culture-derived endometrial bovine (BEC) cells and the field BoGHV4 strain 07/435 were used. Supernatants and cells were collected at 12, 24, and 48 hours post infection (hpi) for virus titration using the endpoint titration method. A factorial model, as a function of time and treatment, testing hypotheses of absence of interaction was used. Viral titers (VT) were fitted to polynomial regression models. The extracellular VT in BEC cells were not affected by the treatment at any time-points. However, intracellular titers at 24 hpi were significantly higher with 5% PRP and decreased with 10% PRP. At 72 hpi, the VT with PRP significantly decreased compared to earlier time-points. Extracellular BoGHV-4 titers in MDBK, were significantly higher with 5%PRP at all times. Conversely, with 10%PRP, the VT decreased at 48 hpi. However, a significant increase was observed at 72 hpi. This study demonstrates that PRP has strong effects on the replication kinetics of BoGHV4, being highly dependent on the host cell.
Fil: López, Sofía. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Andreoli, Valentina. Università di Parma; Italia
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Grolli, Stefano. Università di Parma; Italia
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Ignacio. Laboratorio Álvarez; Argentina
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
6th International Conference of the European College of Veterinary Microbiology
Nottingham
Reino Unido
European College of Veterinary Microbiology
University of Nottingham
Materia
Platelet rich plasma
BoGHV-4
Replication
Endometrial cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258364
Acceder
id CONICETDig_007e07f0f806bd15ff06d9423f565e66
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitroLópez, SofíaAndreoli, ValentinaPereyra, Susana BeatrizRomeo, FlorenciaDelgado, Santiago GermánGrolli, StefanoPerez, SandraAlvarez, IgnacioVerna, Andrea ElizabethPlatelet rich plasmaBoGHV-4ReplicationEndometrial cellshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Diseases of the reproductive tract are a frequent problem in dairy herds. Herpesviruses cause several syndromes including uterine diseases. The role of bovine gammaherpesvirus 4 (BoGHV-4) in the development of endometritis has not been clearly described. Platelet-rich plasma (PRP) is an emerging therapeutic in tissue regeneration due to its enrichment in growth factors with mitogenic and anti-inflammatory potential. This study analysed the replication kinetics of BoGHV-4 in the presence of 5% and 10% PRP, compared to 10% fetal bovine serum. For this, Madin-Darby Bovine Kidney (MDBK) and primary culture-derived endometrial bovine (BEC) cells and the field BoGHV4 strain 07/435 were used. Supernatants and cells were collected at 12, 24, and 48 hours post infection (hpi) for virus titration using the endpoint titration method. A factorial model, as a function of time and treatment, testing hypotheses of absence of interaction was used. Viral titers (VT) were fitted to polynomial regression models. The extracellular VT in BEC cells were not affected by the treatment at any time-points. However, intracellular titers at 24 hpi were significantly higher with 5% PRP and decreased with 10% PRP. At 72 hpi, the VT with PRP significantly decreased compared to earlier time-points. Extracellular BoGHV-4 titers in MDBK, were significantly higher with 5%PRP at all times. Conversely, with 10%PRP, the VT decreased at 48 hpi. However, a significant increase was observed at 72 hpi. This study demonstrates that PRP has strong effects on the replication kinetics of BoGHV4, being highly dependent on the host cell.Fil: López, Sofía. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Andreoli, Valentina. Università di Parma; ItaliaFil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Grolli, Stefano. Università di Parma; ItaliaFil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Alvarez, Ignacio. Laboratorio Álvarez; ArgentinaFil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina6th International Conference of the European College of Veterinary MicrobiologyNottinghamReino UnidoEuropean College of Veterinary MicrobiologyUniversity of NottinghamEuropean College of Veterinary Microbiology2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/258364Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro; 6th International Conference of the European College of Veterinary Microbiology; Nottingham; Reino Unido; 2024; 48-49CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://clearwaterevents.eventscase.com/EN/6InternationalConferenceECVMNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:17Zoai:ri.conicet.gov.ar:11336/258364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:17.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
title Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
spellingShingle Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
López, Sofía
Platelet rich plasma
BoGHV-4
Replication
Endometrial cells
title_short Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
title_full Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
title_fullStr Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
title_full_unstemmed Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
title_sort Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro
dc.creator.none.fl_str_mv López, Sofía
Andreoli, Valentina
Pereyra, Susana Beatriz
Romeo, Florencia
Delgado, Santiago Germán
Grolli, Stefano
Perez, Sandra
Alvarez, Ignacio
Verna, Andrea Elizabeth
author López, Sofía
author_facet López, Sofía
Andreoli, Valentina
Pereyra, Susana Beatriz
Romeo, Florencia
Delgado, Santiago Germán
Grolli, Stefano
Perez, Sandra
Alvarez, Ignacio
Verna, Andrea Elizabeth
author_role author
author2 Andreoli, Valentina
Pereyra, Susana Beatriz
Romeo, Florencia
Delgado, Santiago Germán
Grolli, Stefano
Perez, Sandra
Alvarez, Ignacio
Verna, Andrea Elizabeth
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Platelet rich plasma
BoGHV-4
Replication
Endometrial cells
topic Platelet rich plasma
BoGHV-4
Replication
Endometrial cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Diseases of the reproductive tract are a frequent problem in dairy herds. Herpesviruses cause several syndromes including uterine diseases. The role of bovine gammaherpesvirus 4 (BoGHV-4) in the development of endometritis has not been clearly described. Platelet-rich plasma (PRP) is an emerging therapeutic in tissue regeneration due to its enrichment in growth factors with mitogenic and anti-inflammatory potential. This study analysed the replication kinetics of BoGHV-4 in the presence of 5% and 10% PRP, compared to 10% fetal bovine serum. For this, Madin-Darby Bovine Kidney (MDBK) and primary culture-derived endometrial bovine (BEC) cells and the field BoGHV4 strain 07/435 were used. Supernatants and cells were collected at 12, 24, and 48 hours post infection (hpi) for virus titration using the endpoint titration method. A factorial model, as a function of time and treatment, testing hypotheses of absence of interaction was used. Viral titers (VT) were fitted to polynomial regression models. The extracellular VT in BEC cells were not affected by the treatment at any time-points. However, intracellular titers at 24 hpi were significantly higher with 5% PRP and decreased with 10% PRP. At 72 hpi, the VT with PRP significantly decreased compared to earlier time-points. Extracellular BoGHV-4 titers in MDBK, were significantly higher with 5%PRP at all times. Conversely, with 10%PRP, the VT decreased at 48 hpi. However, a significant increase was observed at 72 hpi. This study demonstrates that PRP has strong effects on the replication kinetics of BoGHV4, being highly dependent on the host cell.
Fil: López, Sofía. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Andreoli, Valentina. Università di Parma; Italia
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Grolli, Stefano. Università di Parma; Italia
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Ignacio. Laboratorio Álvarez; Argentina
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
6th International Conference of the European College of Veterinary Microbiology
Nottingham
Reino Unido
European College of Veterinary Microbiology
University of Nottingham
description Diseases of the reproductive tract are a frequent problem in dairy herds. Herpesviruses cause several syndromes including uterine diseases. The role of bovine gammaherpesvirus 4 (BoGHV-4) in the development of endometritis has not been clearly described. Platelet-rich plasma (PRP) is an emerging therapeutic in tissue regeneration due to its enrichment in growth factors with mitogenic and anti-inflammatory potential. This study analysed the replication kinetics of BoGHV-4 in the presence of 5% and 10% PRP, compared to 10% fetal bovine serum. For this, Madin-Darby Bovine Kidney (MDBK) and primary culture-derived endometrial bovine (BEC) cells and the field BoGHV4 strain 07/435 were used. Supernatants and cells were collected at 12, 24, and 48 hours post infection (hpi) for virus titration using the endpoint titration method. A factorial model, as a function of time and treatment, testing hypotheses of absence of interaction was used. Viral titers (VT) were fitted to polynomial regression models. The extracellular VT in BEC cells were not affected by the treatment at any time-points. However, intracellular titers at 24 hpi were significantly higher with 5% PRP and decreased with 10% PRP. At 72 hpi, the VT with PRP significantly decreased compared to earlier time-points. Extracellular BoGHV-4 titers in MDBK, were significantly higher with 5%PRP at all times. Conversely, with 10%PRP, the VT decreased at 48 hpi. However, a significant increase was observed at 72 hpi. This study demonstrates that PRP has strong effects on the replication kinetics of BoGHV4, being highly dependent on the host cell.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258364
Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro; 6th International Conference of the European College of Veterinary Microbiology; Nottingham; Reino Unido; 2024; 48-49
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258364
identifier_str_mv Replication kinetics of bovine gammaherpesvirus 4 in the presence of platelet rich plasma in vitro; 6th International Conference of the European College of Veterinary Microbiology; Nottingham; Reino Unido; 2024; 48-49
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://clearwaterevents.eventscase.com/EN/6InternationalConferenceECVM
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv European College of Veterinary Microbiology
publisher.none.fl_str_mv European College of Veterinary Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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