Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma

Autores
López, Sofía; Álvarez, Ignacio; Andreoli, Valentina; Delgado, Santiago Germán; Perez, Sandra; Pereyra, Susana Beatriz; Romeo, Florencia; Grolli, Stefano; Verna, Andrea Elizabeth
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-α gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate–early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFNγ expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments.
Fil: López, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Álvarez, Ignacio. Sveriges Lantbruksuniversitet (slu);
Fil: Andreoli, Valentina. Università di Torino; Italia
Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Grolli, Stefano. Università di Parma; Italia
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Materia
PLATELET RICH PLASMA
CATTLE
REGENERATIVE MEDICINE
BOVINE GAMMAHERPESVIRUS 4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263673
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasmaLópez, SofíaÁlvarez, IgnacioAndreoli, ValentinaDelgado, Santiago GermánPerez, SandraPereyra, Susana BeatrizRomeo, FlorenciaGrolli, StefanoVerna, Andrea ElizabethPLATELET RICH PLASMACATTLEREGENERATIVE MEDICINEBOVINE GAMMAHERPESVIRUS 4https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-α gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate–early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFNγ expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments.Fil: López, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Álvarez, Ignacio. Sveriges Lantbruksuniversitet (slu);Fil: Andreoli, Valentina. Università di Torino; ItaliaFil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Grolli, Stefano. Università di Parma; ItaliaFil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaMultidisciplinary Digital Publishing Institute2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263673López, Sofía; Álvarez, Ignacio; Andreoli, Valentina; Delgado, Santiago Germán; Perez, Sandra; et al.; Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma; Multidisciplinary Digital Publishing Institute; Viruses; 17; 6; 5-2025; 1-131999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/17/6/744info:eu-repo/semantics/altIdentifier/doi/10.3390/v17060744info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:14Zoai:ri.conicet.gov.ar:11336/263673instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:14.922CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
title Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
spellingShingle Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
López, Sofía
PLATELET RICH PLASMA
CATTLE
REGENERATIVE MEDICINE
BOVINE GAMMAHERPESVIRUS 4
title_short Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
title_full Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
title_fullStr Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
title_full_unstemmed Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
title_sort Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma
dc.creator.none.fl_str_mv López, Sofía
Álvarez, Ignacio
Andreoli, Valentina
Delgado, Santiago Germán
Perez, Sandra
Pereyra, Susana Beatriz
Romeo, Florencia
Grolli, Stefano
Verna, Andrea Elizabeth
author López, Sofía
author_facet López, Sofía
Álvarez, Ignacio
Andreoli, Valentina
Delgado, Santiago Germán
Perez, Sandra
Pereyra, Susana Beatriz
Romeo, Florencia
Grolli, Stefano
Verna, Andrea Elizabeth
author_role author
author2 Álvarez, Ignacio
Andreoli, Valentina
Delgado, Santiago Germán
Perez, Sandra
Pereyra, Susana Beatriz
Romeo, Florencia
Grolli, Stefano
Verna, Andrea Elizabeth
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLATELET RICH PLASMA
CATTLE
REGENERATIVE MEDICINE
BOVINE GAMMAHERPESVIRUS 4
topic PLATELET RICH PLASMA
CATTLE
REGENERATIVE MEDICINE
BOVINE GAMMAHERPESVIRUS 4
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-α gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate–early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFNγ expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments.
Fil: López, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Álvarez, Ignacio. Sveriges Lantbruksuniversitet (slu);
Fil: Andreoli, Valentina. Università di Torino; Italia
Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Grolli, Stefano. Università di Parma; Italia
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
description Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-α gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate–early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFNγ expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263673
López, Sofía; Álvarez, Ignacio; Andreoli, Valentina; Delgado, Santiago Germán; Perez, Sandra; et al.; Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma; Multidisciplinary Digital Publishing Institute; Viruses; 17; 6; 5-2025; 1-13
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263673
identifier_str_mv López, Sofía; Álvarez, Ignacio; Andreoli, Valentina; Delgado, Santiago Germán; Perez, Sandra; et al.; Gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet-rich plasma; Multidisciplinary Digital Publishing Institute; Viruses; 17; 6; 5-2025; 1-13
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/17/6/744
info:eu-repo/semantics/altIdentifier/doi/10.3390/v17060744
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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