Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood
- Autores
- Vieira, Alexandre R.; Deeley, Kathleen B.; Callahan, Nicholas F.; Noel, Jacqueline B.; Anjomshoaa, Ida; Carricato, Wendy M.; Schulhof, Louise P.; DeSensi, Rebecca S.; Gandhi, Pooja; Resick, Judith M.; Brandon, Carla A.; Rozhon, Christopher; Patir, Asli; Yildirim, Mine; Poletta, Fernando Adrián; Mereb, Juan C.; Letra, Ariadne; Menezes, Renato; Wendell, Steven; López Camelo, Jorge Santiago; Castilla, Eduardo Enrique; Orioli, Ieda Maria; Seymen, Figen; Weyant, Robert J.; Crout, Richard; McNeil, Daniel W.; Modesto, Adriana; Marazita, Mary L.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.
Fil: Vieira, Alexandre R.. University of Pittsburgh; Estados Unidos
Fil: Deeley, Kathleen B.. University of Pittsburgh; Estados Unidos
Fil: Callahan, Nicholas F.. University of Pittsburgh; Estados Unidos
Fil: Noel, Jacqueline B.. University of Pittsburgh; Estados Unidos
Fil: Anjomshoaa, Ida. University of Pittsburgh; Estados Unidos
Fil: Carricato, Wendy M.. University of Pittsburgh; Estados Unidos
Fil: Schulhof, Louise P.. University of Pittsburgh; Estados Unidos
Fil: DeSensi, Rebecca S.. University of Pittsburgh; Estados Unidos
Fil: Gandhi, Pooja. University of Pittsburgh; Estados Unidos
Fil: Resick, Judith M.. University of Pittsburgh; Estados Unidos
Fil: Brandon, Carla A.. University of Pittsburgh; Estados Unidos
Fil: Rozhon, Christopher. University of Pittsburgh; Estados Unidos
Fil: Patir, Asli. Istanbul Medipol University; Turquía
Fil: Yildirim, Mine. Istanbul University; Turquía
Fil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Mereb, Juan C.. Hospital de Area El Bolsón; Argentina
Fil: Letra, Ariadne. University of Pittsburgh; Estados Unidos
Fil: Menezes, Renato. University of Pittsburgh; Estados Unidos
Fil: Wendell, Steven. University of Pittsburgh; Estados Unidos
Fil: López Camelo, Jorge Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Castilla, Eduardo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Orioli, Ieda Maria. Hospital de Area El Bolsón; Argentina
Fil: Seymen, Figen. Istanbul Universit; Turquía
Fil: Weyant, Robert J.. University of Pittsburgh; Estados Unidos
Fil: Crout, Richard. West Virginia University; Estados Unidos
Fil: McNeil, Daniel W.. West Virginia University; Estados Unidos
Fil: Modesto, Adriana. University of Pittsburgh; Estados Unidos
Fil: Marazita, Mary L.. University of Pittsburgh; Estados Unidos - Materia
-
Detection
Streptococcus
Genomic
DNA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/125105
Ver los metadatos del registro completo
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Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and BloodVieira, Alexandre R.Deeley, Kathleen B.Callahan, Nicholas F.Noel, Jacqueline B.Anjomshoaa, IdaCarricato, Wendy M.Schulhof, Louise P.DeSensi, Rebecca S.Gandhi, PoojaResick, Judith M.Brandon, Carla A.Rozhon, ChristopherPatir, AsliYildirim, MinePoletta, Fernando AdriánMereb, Juan C.Letra, AriadneMenezes, RenatoWendell, StevenLópez Camelo, Jorge SantiagoCastilla, Eduardo EnriqueOrioli, Ieda MariaSeymen, FigenWeyant, Robert J.Crout, RichardMcNeil, Daniel W.Modesto, AdrianaMarazita, Mary L.DetectionStreptococcusGenomicDNAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.Fil: Vieira, Alexandre R.. University of Pittsburgh; Estados UnidosFil: Deeley, Kathleen B.. University of Pittsburgh; Estados UnidosFil: Callahan, Nicholas F.. University of Pittsburgh; Estados UnidosFil: Noel, Jacqueline B.. University of Pittsburgh; Estados UnidosFil: Anjomshoaa, Ida. University of Pittsburgh; Estados UnidosFil: Carricato, Wendy M.. University of Pittsburgh; Estados UnidosFil: Schulhof, Louise P.. University of Pittsburgh; Estados UnidosFil: DeSensi, Rebecca S.. University of Pittsburgh; Estados UnidosFil: Gandhi, Pooja. University of Pittsburgh; Estados UnidosFil: Resick, Judith M.. University of Pittsburgh; Estados UnidosFil: Brandon, Carla A.. University of Pittsburgh; Estados UnidosFil: Rozhon, Christopher. University of Pittsburgh; Estados UnidosFil: Patir, Asli. Istanbul Medipol University; TurquíaFil: Yildirim, Mine. Istanbul University; TurquíaFil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Mereb, Juan C.. Hospital de Area El Bolsón; ArgentinaFil: Letra, Ariadne. University of Pittsburgh; Estados UnidosFil: Menezes, Renato. University of Pittsburgh; Estados UnidosFil: Wendell, Steven. University of Pittsburgh; Estados UnidosFil: López Camelo, Jorge Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Castilla, Eduardo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Orioli, Ieda Maria. Hospital de Area El Bolsón; ArgentinaFil: Seymen, Figen. Istanbul Universit; TurquíaFil: Weyant, Robert J.. University of Pittsburgh; Estados UnidosFil: Crout, Richard. West Virginia University; Estados UnidosFil: McNeil, Daniel W.. West Virginia University; Estados UnidosFil: Modesto, Adriana. University of Pittsburgh; Estados UnidosFil: Marazita, Mary L.. University of Pittsburgh; Estados UnidosF D I World Dental Press Ltd2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125105Vieira, Alexandre R.; Deeley, Kathleen B.; Callahan, Nicholas F.; Noel, Jacqueline B.; Anjomshoaa, Ida; et al.; Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood; F D I World Dental Press Ltd; International Dental Journal - (Online); 2011; 4-2011; 1-62090-438X0020-6539CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128887/info:eu-repo/semantics/altIdentifier/doi/10.5402%2F2011%2F543561info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/isrn/2011/543561/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:43:18Zoai:ri.conicet.gov.ar:11336/125105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:43:19.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood |
| title |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood |
| spellingShingle |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood Vieira, Alexandre R. Detection Streptococcus Genomic DNA |
| title_short |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood |
| title_full |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood |
| title_fullStr |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood |
| title_full_unstemmed |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood |
| title_sort |
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood |
| dc.creator.none.fl_str_mv |
Vieira, Alexandre R. Deeley, Kathleen B. Callahan, Nicholas F. Noel, Jacqueline B. Anjomshoaa, Ida Carricato, Wendy M. Schulhof, Louise P. DeSensi, Rebecca S. Gandhi, Pooja Resick, Judith M. Brandon, Carla A. Rozhon, Christopher Patir, Asli Yildirim, Mine Poletta, Fernando Adrián Mereb, Juan C. Letra, Ariadne Menezes, Renato Wendell, Steven López Camelo, Jorge Santiago Castilla, Eduardo Enrique Orioli, Ieda Maria Seymen, Figen Weyant, Robert J. Crout, Richard McNeil, Daniel W. Modesto, Adriana Marazita, Mary L. |
| author |
Vieira, Alexandre R. |
| author_facet |
Vieira, Alexandre R. Deeley, Kathleen B. Callahan, Nicholas F. Noel, Jacqueline B. Anjomshoaa, Ida Carricato, Wendy M. Schulhof, Louise P. DeSensi, Rebecca S. Gandhi, Pooja Resick, Judith M. Brandon, Carla A. Rozhon, Christopher Patir, Asli Yildirim, Mine Poletta, Fernando Adrián Mereb, Juan C. Letra, Ariadne Menezes, Renato Wendell, Steven López Camelo, Jorge Santiago Castilla, Eduardo Enrique Orioli, Ieda Maria Seymen, Figen Weyant, Robert J. Crout, Richard McNeil, Daniel W. Modesto, Adriana Marazita, Mary L. |
| author_role |
author |
| author2 |
Deeley, Kathleen B. Callahan, Nicholas F. Noel, Jacqueline B. Anjomshoaa, Ida Carricato, Wendy M. Schulhof, Louise P. DeSensi, Rebecca S. Gandhi, Pooja Resick, Judith M. Brandon, Carla A. Rozhon, Christopher Patir, Asli Yildirim, Mine Poletta, Fernando Adrián Mereb, Juan C. Letra, Ariadne Menezes, Renato Wendell, Steven López Camelo, Jorge Santiago Castilla, Eduardo Enrique Orioli, Ieda Maria Seymen, Figen Weyant, Robert J. Crout, Richard McNeil, Daniel W. Modesto, Adriana Marazita, Mary L. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Detection Streptococcus Genomic DNA |
| topic |
Detection Streptococcus Genomic DNA |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults. Fil: Vieira, Alexandre R.. University of Pittsburgh; Estados Unidos Fil: Deeley, Kathleen B.. University of Pittsburgh; Estados Unidos Fil: Callahan, Nicholas F.. University of Pittsburgh; Estados Unidos Fil: Noel, Jacqueline B.. University of Pittsburgh; Estados Unidos Fil: Anjomshoaa, Ida. University of Pittsburgh; Estados Unidos Fil: Carricato, Wendy M.. University of Pittsburgh; Estados Unidos Fil: Schulhof, Louise P.. University of Pittsburgh; Estados Unidos Fil: DeSensi, Rebecca S.. University of Pittsburgh; Estados Unidos Fil: Gandhi, Pooja. University of Pittsburgh; Estados Unidos Fil: Resick, Judith M.. University of Pittsburgh; Estados Unidos Fil: Brandon, Carla A.. University of Pittsburgh; Estados Unidos Fil: Rozhon, Christopher. University of Pittsburgh; Estados Unidos Fil: Patir, Asli. Istanbul Medipol University; Turquía Fil: Yildirim, Mine. Istanbul University; Turquía Fil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina Fil: Mereb, Juan C.. Hospital de Area El Bolsón; Argentina Fil: Letra, Ariadne. University of Pittsburgh; Estados Unidos Fil: Menezes, Renato. University of Pittsburgh; Estados Unidos Fil: Wendell, Steven. University of Pittsburgh; Estados Unidos Fil: López Camelo, Jorge Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina Fil: Castilla, Eduardo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina Fil: Orioli, Ieda Maria. Hospital de Area El Bolsón; Argentina Fil: Seymen, Figen. Istanbul Universit; Turquía Fil: Weyant, Robert J.. University of Pittsburgh; Estados Unidos Fil: Crout, Richard. West Virginia University; Estados Unidos Fil: McNeil, Daniel W.. West Virginia University; Estados Unidos Fil: Modesto, Adriana. University of Pittsburgh; Estados Unidos Fil: Marazita, Mary L.. University of Pittsburgh; Estados Unidos |
| description |
Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-04 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
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http://hdl.handle.net/11336/125105 Vieira, Alexandre R.; Deeley, Kathleen B.; Callahan, Nicholas F.; Noel, Jacqueline B.; Anjomshoaa, Ida; et al.; Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood; F D I World Dental Press Ltd; International Dental Journal - (Online); 2011; 4-2011; 1-6 2090-438X 0020-6539 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/125105 |
| identifier_str_mv |
Vieira, Alexandre R.; Deeley, Kathleen B.; Callahan, Nicholas F.; Noel, Jacqueline B.; Anjomshoaa, Ida; et al.; Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood; F D I World Dental Press Ltd; International Dental Journal - (Online); 2011; 4-2011; 1-6 2090-438X 0020-6539 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
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openAccess |
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application/pdf application/pdf application/pdf |
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F D I World Dental Press Ltd |
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F D I World Dental Press Ltd |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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