P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)

Autores
Gallardo, Guillermina Ana; Clemente, Valentina; Ibarra, Agustina; Facchinetti, Maria Marta; Curino, Alejandro Carlos; Fermento, María Eugenia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Triple-negative breast cancer (TNBC) is a heterogeneous group of tumors that lack specific molecular targets. Therefore, it is neces- sary to investigate potential tumor markers for this subtype of BC. There are few studies in TNBC on the role of p300 in apoptosis and they are contradictory. Previously, we published that pharma- cological inhibition of p300 increases apoptosis in murine BC cells, however, it is necessary to check whether the same effect occurs in human TNBC cells. Recently a prometastatic role of p300 in BC has been found. In addition, we demonstrated that pharmacological inhi- bition of p300 decreases migration and adhesion in TNBC, showing the need to delve into the molecules involved. Hence, the aim of this work was to study the effect of the inhibition of the acetylase func- tion of p300 in the processes of apoptosis and metastasis in human TNBC cells. The MDA-MB-231 cell line was treated with VV59 (in- hibitor of the acetylase function of p300) or its vehicle (DMSO). In a cell viability assay, we observed that the pharmacological inhibition of p300 produced a decrease in the number of cells compared to vehicle (p<0.001). When we analyzed the cell cycle by flow cytome- try, we detected an increase in the sub G0 phase and a decrease in the G0/G1 phase in the cells treated with VV59 compared to those treated with the vehicle (p˂0.001). We also observed an increase in apoptotic morphology in VV59-treated-cells compared to vehi- cle-treated cells by dapi staining (p˂0.001). On the other hand, by immunofluorescence assay we detected that pharmacological inhi- bition of p300 induced an increase in the levels of E-cadherin and β-catenin in the membrane, and a decrease in the number of stress fibers compared with the control cells (p˂0.01). Taken together, these results demonstrate an antitumor role for pharmacological inhibition of p300 acetylase function in TNBC.
Fil: Gallardo, Guillermina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Clemente, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ibarra, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
on line
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
P300
CANCER
MAMA
TRIPLE NEGATIVO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228097
Acceder
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spelling P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)Gallardo, Guillermina AnaClemente, ValentinaIbarra, AgustinaFacchinetti, Maria MartaCurino, Alejandro CarlosFermento, María EugeniaP300CANCERMAMATRIPLE NEGATIVOhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Triple-negative breast cancer (TNBC) is a heterogeneous group of tumors that lack specific molecular targets. Therefore, it is neces- sary to investigate potential tumor markers for this subtype of BC. There are few studies in TNBC on the role of p300 in apoptosis and they are contradictory. Previously, we published that pharma- cological inhibition of p300 increases apoptosis in murine BC cells, however, it is necessary to check whether the same effect occurs in human TNBC cells. Recently a prometastatic role of p300 in BC has been found. In addition, we demonstrated that pharmacological inhi- bition of p300 decreases migration and adhesion in TNBC, showing the need to delve into the molecules involved. Hence, the aim of this work was to study the effect of the inhibition of the acetylase func- tion of p300 in the processes of apoptosis and metastasis in human TNBC cells. The MDA-MB-231 cell line was treated with VV59 (in- hibitor of the acetylase function of p300) or its vehicle (DMSO). In a cell viability assay, we observed that the pharmacological inhibition of p300 produced a decrease in the number of cells compared to vehicle (p<0.001). When we analyzed the cell cycle by flow cytome- try, we detected an increase in the sub G0 phase and a decrease in the G0/G1 phase in the cells treated with VV59 compared to those treated with the vehicle (p˂0.001). We also observed an increase in apoptotic morphology in VV59-treated-cells compared to vehi- cle-treated cells by dapi staining (p˂0.001). On the other hand, by immunofluorescence assay we detected that pharmacological inhi- bition of p300 induced an increase in the levels of E-cadherin and β-catenin in the membrane, and a decrease in the number of stress fibers compared with the control cells (p˂0.01). Taken together, these results demonstrate an antitumor role for pharmacological inhibition of p300 acetylase function in TNBC.Fil: Gallardo, Guillermina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Clemente, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Ibarra, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiologíaon lineArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228097P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC); LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; on line; Argentina; 2020; 122-1220025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:20Zoai:ri.conicet.gov.ar:11336/228097instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:20.564CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
title P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
spellingShingle P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
Gallardo, Guillermina Ana
P300
CANCER
MAMA
TRIPLE NEGATIVO
title_short P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
title_full P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
title_fullStr P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
title_full_unstemmed P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
title_sort P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC)
dc.creator.none.fl_str_mv Gallardo, Guillermina Ana
Clemente, Valentina
Ibarra, Agustina
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Fermento, María Eugenia
author Gallardo, Guillermina Ana
author_facet Gallardo, Guillermina Ana
Clemente, Valentina
Ibarra, Agustina
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Fermento, María Eugenia
author_role author
author2 Clemente, Valentina
Ibarra, Agustina
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Fermento, María Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv P300
CANCER
MAMA
TRIPLE NEGATIVO
topic P300
CANCER
MAMA
TRIPLE NEGATIVO
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Triple-negative breast cancer (TNBC) is a heterogeneous group of tumors that lack specific molecular targets. Therefore, it is neces- sary to investigate potential tumor markers for this subtype of BC. There are few studies in TNBC on the role of p300 in apoptosis and they are contradictory. Previously, we published that pharma- cological inhibition of p300 increases apoptosis in murine BC cells, however, it is necessary to check whether the same effect occurs in human TNBC cells. Recently a prometastatic role of p300 in BC has been found. In addition, we demonstrated that pharmacological inhi- bition of p300 decreases migration and adhesion in TNBC, showing the need to delve into the molecules involved. Hence, the aim of this work was to study the effect of the inhibition of the acetylase func- tion of p300 in the processes of apoptosis and metastasis in human TNBC cells. The MDA-MB-231 cell line was treated with VV59 (in- hibitor of the acetylase function of p300) or its vehicle (DMSO). In a cell viability assay, we observed that the pharmacological inhibition of p300 produced a decrease in the number of cells compared to vehicle (p<0.001). When we analyzed the cell cycle by flow cytome- try, we detected an increase in the sub G0 phase and a decrease in the G0/G1 phase in the cells treated with VV59 compared to those treated with the vehicle (p˂0.001). We also observed an increase in apoptotic morphology in VV59-treated-cells compared to vehi- cle-treated cells by dapi staining (p˂0.001). On the other hand, by immunofluorescence assay we detected that pharmacological inhi- bition of p300 induced an increase in the levels of E-cadherin and β-catenin in the membrane, and a decrease in the number of stress fibers compared with the control cells (p˂0.01). Taken together, these results demonstrate an antitumor role for pharmacological inhibition of p300 acetylase function in TNBC.
Fil: Gallardo, Guillermina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Clemente, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ibarra, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
on line
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Triple-negative breast cancer (TNBC) is a heterogeneous group of tumors that lack specific molecular targets. Therefore, it is neces- sary to investigate potential tumor markers for this subtype of BC. There are few studies in TNBC on the role of p300 in apoptosis and they are contradictory. Previously, we published that pharma- cological inhibition of p300 increases apoptosis in murine BC cells, however, it is necessary to check whether the same effect occurs in human TNBC cells. Recently a prometastatic role of p300 in BC has been found. In addition, we demonstrated that pharmacological inhi- bition of p300 decreases migration and adhesion in TNBC, showing the need to delve into the molecules involved. Hence, the aim of this work was to study the effect of the inhibition of the acetylase func- tion of p300 in the processes of apoptosis and metastasis in human TNBC cells. The MDA-MB-231 cell line was treated with VV59 (in- hibitor of the acetylase function of p300) or its vehicle (DMSO). In a cell viability assay, we observed that the pharmacological inhibition of p300 produced a decrease in the number of cells compared to vehicle (p<0.001). When we analyzed the cell cycle by flow cytome- try, we detected an increase in the sub G0 phase and a decrease in the G0/G1 phase in the cells treated with VV59 compared to those treated with the vehicle (p˂0.001). We also observed an increase in apoptotic morphology in VV59-treated-cells compared to vehi- cle-treated cells by dapi staining (p˂0.001). On the other hand, by immunofluorescence assay we detected that pharmacological inhi- bition of p300 induced an increase in the levels of E-cadherin and β-catenin in the membrane, and a decrease in the number of stress fibers compared with the control cells (p˂0.01). Taken together, these results demonstrate an antitumor role for pharmacological inhibition of p300 acetylase function in TNBC.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228097
P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC); LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; on line; Argentina; 2020; 122-122
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228097
identifier_str_mv P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC); LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; on line; Argentina; 2020; 122-122
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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