Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars

Autores
Carreras, Claudia; Zamorano, Alan; Villalobos González, Luis; Pimentel, Paula; Pizarro, Lorena; Beltrán, María Francisca; Cui, Weier; Pinto, Manuel; Figueroa, Franco; Rubilar Hernández, Carlos; Llanes, Analia Susana; Bertaccini, Assunta; Fiore, Nicola
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas syringae pv. syringae is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to P. syringae pv. syringae, respectively) after 1 and 7 days post-inoculation (dpi) with the bacterium. Symptoms of bacterial canker became evident from the fifth day. At 1 dpi, cultivar Santina showed a faster response to infection and a larger number of differentially expressed genes (DEGs) than cultivar Bing. At 7 dpi, cultivar Bing almost doubled its DEGs, while cultivar Santina tended to the normal DEG levels. P. syringae pv. syringae infection downregulated the expressions of key genes of the photosynthesis process at 1 dpi in the less susceptible cultivar. The results suggest that the difference in susceptibility to P. syringae pv. syringae is linked to the timeliness of pathogen recognition, limiting the bacteria’s dispersion through modeling its cell wall, and regulation of genes encoding photosynthesis pathway. Through this study, it has been possible to progress the knowledge of relevant factors related to the susceptibility of the two studied cherry cultivars to P. syringae pv. syringae.
Fil: Carreras, Claudia. Universidad de Chile; Chile
Fil: Zamorano, Alan. Universidad de Chile; Chile
Fil: Villalobos González, Luis. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Pimentel, Paula. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Pizarro, Lorena. Universidad Bernardo O? Higgins;
Fil: Beltrán, María Francisca. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Cui, Weier. Universidad de Chile; Chile
Fil: Pinto, Manuel. Universidad Bernardo O? Higgins;
Fil: Figueroa, Franco. Universidad Bernardo O? Higgins;
Fil: Rubilar Hernández, Carlos. Universidad Bernardo O? Higgins;
Fil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Bertaccini, Assunta. Universidad de Bologna; Italia
Fil: Fiore, Nicola. Universidad de Chile.; Chile
Materia
PLANT PATHOGENIC BACTERIA
TRANSCRIPTOME
PLANT-PATHOGEN INTERACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262368
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/262368
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry CultivarsCarreras, ClaudiaZamorano, AlanVillalobos González, LuisPimentel, PaulaPizarro, LorenaBeltrán, María FranciscaCui, WeierPinto, ManuelFigueroa, FrancoRubilar Hernández, CarlosLlanes, Analia SusanaBertaccini, AssuntaFiore, NicolaPLANT PATHOGENIC BACTERIATRANSCRIPTOMEPLANT-PATHOGEN INTERACTIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Pseudomonas syringae pv. syringae is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to P. syringae pv. syringae, respectively) after 1 and 7 days post-inoculation (dpi) with the bacterium. Symptoms of bacterial canker became evident from the fifth day. At 1 dpi, cultivar Santina showed a faster response to infection and a larger number of differentially expressed genes (DEGs) than cultivar Bing. At 7 dpi, cultivar Bing almost doubled its DEGs, while cultivar Santina tended to the normal DEG levels. P. syringae pv. syringae infection downregulated the expressions of key genes of the photosynthesis process at 1 dpi in the less susceptible cultivar. The results suggest that the difference in susceptibility to P. syringae pv. syringae is linked to the timeliness of pathogen recognition, limiting the bacteria’s dispersion through modeling its cell wall, and regulation of genes encoding photosynthesis pathway. Through this study, it has been possible to progress the knowledge of relevant factors related to the susceptibility of the two studied cherry cultivars to P. syringae pv. syringae.Fil: Carreras, Claudia. Universidad de Chile; ChileFil: Zamorano, Alan. Universidad de Chile; ChileFil: Villalobos González, Luis. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Pimentel, Paula. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Pizarro, Lorena. Universidad Bernardo O? Higgins;Fil: Beltrán, María Francisca. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Cui, Weier. Universidad de Chile; ChileFil: Pinto, Manuel. Universidad Bernardo O? Higgins;Fil: Figueroa, Franco. Universidad Bernardo O? Higgins;Fil: Rubilar Hernández, Carlos. Universidad Bernardo O? Higgins;Fil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Bertaccini, Assunta. Universidad de Bologna; ItaliaFil: Fiore, Nicola. Universidad de Chile.; ChileMDPI2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262368Carreras, Claudia; Zamorano, Alan; Villalobos González, Luis; Pimentel, Paula; Pizarro, Lorena; et al.; Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars; MDPI; Plants; 14; 1; 12-2024; 1-192223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/14/1/87info:eu-repo/semantics/altIdentifier/doi/10.3390/plants14010087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:01Zoai:ri.conicet.gov.ar:11336/262368instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:01.404CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
title Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
spellingShingle Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
Carreras, Claudia
PLANT PATHOGENIC BACTERIA
TRANSCRIPTOME
PLANT-PATHOGEN INTERACTION
title_short Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
title_full Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
title_fullStr Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
title_full_unstemmed Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
title_sort Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
dc.creator.none.fl_str_mv Carreras, Claudia
Zamorano, Alan
Villalobos González, Luis
Pimentel, Paula
Pizarro, Lorena
Beltrán, María Francisca
Cui, Weier
Pinto, Manuel
Figueroa, Franco
Rubilar Hernández, Carlos
Llanes, Analia Susana
Bertaccini, Assunta
Fiore, Nicola
author Carreras, Claudia
author_facet Carreras, Claudia
Zamorano, Alan
Villalobos González, Luis
Pimentel, Paula
Pizarro, Lorena
Beltrán, María Francisca
Cui, Weier
Pinto, Manuel
Figueroa, Franco
Rubilar Hernández, Carlos
Llanes, Analia Susana
Bertaccini, Assunta
Fiore, Nicola
author_role author
author2 Zamorano, Alan
Villalobos González, Luis
Pimentel, Paula
Pizarro, Lorena
Beltrán, María Francisca
Cui, Weier
Pinto, Manuel
Figueroa, Franco
Rubilar Hernández, Carlos
Llanes, Analia Susana
Bertaccini, Assunta
Fiore, Nicola
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLANT PATHOGENIC BACTERIA
TRANSCRIPTOME
PLANT-PATHOGEN INTERACTION
topic PLANT PATHOGENIC BACTERIA
TRANSCRIPTOME
PLANT-PATHOGEN INTERACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Pseudomonas syringae pv. syringae is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to P. syringae pv. syringae, respectively) after 1 and 7 days post-inoculation (dpi) with the bacterium. Symptoms of bacterial canker became evident from the fifth day. At 1 dpi, cultivar Santina showed a faster response to infection and a larger number of differentially expressed genes (DEGs) than cultivar Bing. At 7 dpi, cultivar Bing almost doubled its DEGs, while cultivar Santina tended to the normal DEG levels. P. syringae pv. syringae infection downregulated the expressions of key genes of the photosynthesis process at 1 dpi in the less susceptible cultivar. The results suggest that the difference in susceptibility to P. syringae pv. syringae is linked to the timeliness of pathogen recognition, limiting the bacteria’s dispersion through modeling its cell wall, and regulation of genes encoding photosynthesis pathway. Through this study, it has been possible to progress the knowledge of relevant factors related to the susceptibility of the two studied cherry cultivars to P. syringae pv. syringae.
Fil: Carreras, Claudia. Universidad de Chile; Chile
Fil: Zamorano, Alan. Universidad de Chile; Chile
Fil: Villalobos González, Luis. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Pimentel, Paula. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Pizarro, Lorena. Universidad Bernardo O? Higgins;
Fil: Beltrán, María Francisca. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Cui, Weier. Universidad de Chile; Chile
Fil: Pinto, Manuel. Universidad Bernardo O? Higgins;
Fil: Figueroa, Franco. Universidad Bernardo O? Higgins;
Fil: Rubilar Hernández, Carlos. Universidad Bernardo O? Higgins;
Fil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Bertaccini, Assunta. Universidad de Bologna; Italia
Fil: Fiore, Nicola. Universidad de Chile.; Chile
description Pseudomonas syringae pv. syringae is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to P. syringae pv. syringae, respectively) after 1 and 7 days post-inoculation (dpi) with the bacterium. Symptoms of bacterial canker became evident from the fifth day. At 1 dpi, cultivar Santina showed a faster response to infection and a larger number of differentially expressed genes (DEGs) than cultivar Bing. At 7 dpi, cultivar Bing almost doubled its DEGs, while cultivar Santina tended to the normal DEG levels. P. syringae pv. syringae infection downregulated the expressions of key genes of the photosynthesis process at 1 dpi in the less susceptible cultivar. The results suggest that the difference in susceptibility to P. syringae pv. syringae is linked to the timeliness of pathogen recognition, limiting the bacteria’s dispersion through modeling its cell wall, and regulation of genes encoding photosynthesis pathway. Through this study, it has been possible to progress the knowledge of relevant factors related to the susceptibility of the two studied cherry cultivars to P. syringae pv. syringae.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262368
Carreras, Claudia; Zamorano, Alan; Villalobos González, Luis; Pimentel, Paula; Pizarro, Lorena; et al.; Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars; MDPI; Plants; 14; 1; 12-2024; 1-19
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262368
identifier_str_mv Carreras, Claudia; Zamorano, Alan; Villalobos González, Luis; Pimentel, Paula; Pizarro, Lorena; et al.; Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars; MDPI; Plants; 14; 1; 12-2024; 1-19
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/14/1/87
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants14010087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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