Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis

Autores
Großkinsky, Dominik K.; Tafner, Richard; Moreno, Maria Virginia; Stenglein, Sebastian Alberto; Garcia, Inés Eugenia; Nelson, Louise M.; Novák, Ondřej; Strnad, Miroslav; Graaff, Eric van der; Roitsch, Thomas
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinindeficientloss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for thisbiocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbebased, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience.
Fil: Großkinsky, Dominik K.. Universidad de Copenhagen; Dinamarca
Fil: Tafner, Richard. University of Graz; Austria
Fil: Moreno, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Garcia, Inés Eugenia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
Fil: Nelson, Louise M.. University of British Columbia; Canadá
Fil: Novák, Ondřej. University Palacky; República Checa
Fil: Strnad, Miroslav. University Palacky; República Checa
Fil: Graaff, Eric van der. Universidad de Copenhagen; Dinamarca. University of Graz; Austria
Fil: Roitsch, Thomas. Czech Globe AS CR; República Checa. University of Graz; Austria. Universidad de Copenhagen; Dinamarca
Materia
Plant beneficial microbes
Cytokinins
Integrated management
Plant protection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29623
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in ArabidopsisGroßkinsky, Dominik K.Tafner, RichardMoreno, Maria VirginiaStenglein, Sebastian AlbertoGarcia, Inés EugeniaNelson, Louise M.Novák, OndřejStrnad, MiroslavGraaff, Eric van derRoitsch, ThomasPlant beneficial microbesCytokininsIntegrated managementPlant protectionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinindeficientloss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for thisbiocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbebased, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience.Fil: Großkinsky, Dominik K.. Universidad de Copenhagen; DinamarcaFil: Tafner, Richard. University of Graz; AustriaFil: Moreno, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Garcia, Inés Eugenia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; ArgentinaFil: Nelson, Louise M.. University of British Columbia; CanadáFil: Novák, Ondřej. University Palacky; República ChecaFil: Strnad, Miroslav. University Palacky; República ChecaFil: Graaff, Eric van der. Universidad de Copenhagen; Dinamarca. University of Graz; AustriaFil: Roitsch, Thomas. Czech Globe AS CR; República Checa. University of Graz; Austria. Universidad de Copenhagen; DinamarcaNature Publishing Group2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29623Großkinsky, Dominik K.; Tafner, Richard; Moreno, Maria Virginia; Stenglein, Sebastian Alberto; Garcia, Inés Eugenia; et al.; Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-11; 233102045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep23310info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep23310info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:28Zoai:ri.conicet.gov.ar:11336/29623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:28.547CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
title Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
spellingShingle Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
Großkinsky, Dominik K.
Plant beneficial microbes
Cytokinins
Integrated management
Plant protection
title_short Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
title_full Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
title_fullStr Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
title_full_unstemmed Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
title_sort Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
dc.creator.none.fl_str_mv Großkinsky, Dominik K.
Tafner, Richard
Moreno, Maria Virginia
Stenglein, Sebastian Alberto
Garcia, Inés Eugenia
Nelson, Louise M.
Novák, Ondřej
Strnad, Miroslav
Graaff, Eric van der
Roitsch, Thomas
author Großkinsky, Dominik K.
author_facet Großkinsky, Dominik K.
Tafner, Richard
Moreno, Maria Virginia
Stenglein, Sebastian Alberto
Garcia, Inés Eugenia
Nelson, Louise M.
Novák, Ondřej
Strnad, Miroslav
Graaff, Eric van der
Roitsch, Thomas
author_role author
author2 Tafner, Richard
Moreno, Maria Virginia
Stenglein, Sebastian Alberto
Garcia, Inés Eugenia
Nelson, Louise M.
Novák, Ondřej
Strnad, Miroslav
Graaff, Eric van der
Roitsch, Thomas
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Plant beneficial microbes
Cytokinins
Integrated management
Plant protection
topic Plant beneficial microbes
Cytokinins
Integrated management
Plant protection
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinindeficientloss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for thisbiocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbebased, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience.
Fil: Großkinsky, Dominik K.. Universidad de Copenhagen; Dinamarca
Fil: Tafner, Richard. University of Graz; Austria
Fil: Moreno, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Garcia, Inés Eugenia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
Fil: Nelson, Louise M.. University of British Columbia; Canadá
Fil: Novák, Ondřej. University Palacky; República Checa
Fil: Strnad, Miroslav. University Palacky; República Checa
Fil: Graaff, Eric van der. Universidad de Copenhagen; Dinamarca. University of Graz; Austria
Fil: Roitsch, Thomas. Czech Globe AS CR; República Checa. University of Graz; Austria. Universidad de Copenhagen; Dinamarca
description Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinindeficientloss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for thisbiocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbebased, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29623
Großkinsky, Dominik K.; Tafner, Richard; Moreno, Maria Virginia; Stenglein, Sebastian Alberto; Garcia, Inés Eugenia; et al.; Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-11; 23310
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29623
identifier_str_mv Großkinsky, Dominik K.; Tafner, Richard; Moreno, Maria Virginia; Stenglein, Sebastian Alberto; Garcia, Inés Eugenia; et al.; Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-11; 23310
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep23310
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep23310
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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