Regulation of iron importers in regions of the central nervous system in iron accumulation models
- Autores
- Giorgi, Gisela; Roque, Marta Elena
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control.
Fil: Giorgi, Gisela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología - Materia
-
BRAIN
IRON
OVERLOAD - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/250857
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Regulation of iron importers in regions of the central nervous system in iron accumulation modelsGiorgi, GiselaRoque, Marta ElenaBRAINIRONOVERLOADhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control.Fil: Giorgi, Gisela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaReunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación revista MedicinaPerez Leiros, Claudia2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/250857Regulation of iron importers in regions of the central nervous system in iron accumulation models; Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 253-2530025-76801669-9106CONICET DigitalCONICETenghttps://www.saic.org.ar/reuniones-anuales-previasinfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previasinfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:34Zoai:ri.conicet.gov.ar:11336/250857instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:35.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Regulation of iron importers in regions of the central nervous system in iron accumulation models |
title |
Regulation of iron importers in regions of the central nervous system in iron accumulation models |
spellingShingle |
Regulation of iron importers in regions of the central nervous system in iron accumulation models Giorgi, Gisela BRAIN IRON OVERLOAD |
title_short |
Regulation of iron importers in regions of the central nervous system in iron accumulation models |
title_full |
Regulation of iron importers in regions of the central nervous system in iron accumulation models |
title_fullStr |
Regulation of iron importers in regions of the central nervous system in iron accumulation models |
title_full_unstemmed |
Regulation of iron importers in regions of the central nervous system in iron accumulation models |
title_sort |
Regulation of iron importers in regions of the central nervous system in iron accumulation models |
dc.creator.none.fl_str_mv |
Giorgi, Gisela Roque, Marta Elena |
author |
Giorgi, Gisela |
author_facet |
Giorgi, Gisela Roque, Marta Elena |
author_role |
author |
author2 |
Roque, Marta Elena |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Perez Leiros, Claudia |
dc.subject.none.fl_str_mv |
BRAIN IRON OVERLOAD |
topic |
BRAIN IRON OVERLOAD |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control. Fil: Giorgi, Gisela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Inmunología Sociedad Argentina de Fisiología |
description |
The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/250857 Regulation of iron importers in regions of the central nervous system in iron accumulation models; Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 253-253 0025-7680 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/250857 |
identifier_str_mv |
Regulation of iron importers in regions of the central nervous system in iron accumulation models; Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 253-253 0025-7680 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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https://www.saic.org.ar/reuniones-anuales-previas info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previas info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/ |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Fundación revista Medicina |
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Fundación revista Medicina |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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