Regulation of iron importers in regions of the central nervous system in iron accumulation models

Autores
Giorgi, Gisela; Roque, Marta Elena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control.
Fil: Giorgi, Gisela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
BRAIN
IRON
OVERLOAD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/250857
Acceder
id CONICETDig_cb19c668d872e1dca0fc39c1eeb30537
oai_identifier_str oai:ri.conicet.gov.ar:11336/250857
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of iron importers in regions of the central nervous system in iron accumulation modelsGiorgi, GiselaRoque, Marta ElenaBRAINIRONOVERLOADhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control.Fil: Giorgi, Gisela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaReunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación revista MedicinaPerez Leiros, Claudia2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/250857Regulation of iron importers in regions of the central nervous system in iron accumulation models; Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 253-2530025-76801669-9106CONICET DigitalCONICETenghttps://www.saic.org.ar/reuniones-anuales-previasinfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previasinfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:57:19Zoai:ri.conicet.gov.ar:11336/250857instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:57:19.918CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of iron importers in regions of the central nervous system in iron accumulation models
title Regulation of iron importers in regions of the central nervous system in iron accumulation models
spellingShingle Regulation of iron importers in regions of the central nervous system in iron accumulation models
Giorgi, Gisela
BRAIN
IRON
OVERLOAD
title_short Regulation of iron importers in regions of the central nervous system in iron accumulation models
title_full Regulation of iron importers in regions of the central nervous system in iron accumulation models
title_fullStr Regulation of iron importers in regions of the central nervous system in iron accumulation models
title_full_unstemmed Regulation of iron importers in regions of the central nervous system in iron accumulation models
title_sort Regulation of iron importers in regions of the central nervous system in iron accumulation models
dc.creator.none.fl_str_mv Giorgi, Gisela
Roque, Marta Elena
author Giorgi, Gisela
author_facet Giorgi, Gisela
Roque, Marta Elena
author_role author
author2 Roque, Marta Elena
author2_role author
dc.contributor.none.fl_str_mv Perez Leiros, Claudia
dc.subject.none.fl_str_mv BRAIN
IRON
OVERLOAD
topic BRAIN
IRON
OVERLOAD
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control.
Fil: Giorgi, Gisela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description The accumulation of the iron excess in brain is frequently detected in neurodegenerative disorders. Therefore, an understanding of the iron proteins regulation in brain could help for the treatment or prevention of neurodegenerative diseases. Objective: study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-likeProtein14 (ZIP14) expressions in mice brain and in human neuroblastoma cells. Materials and Methods: In vivo studies: CF1 mice(25±5g) were divided into 2 groups (n=6/group;paired design):1)Iron-overload: Fe-Saccharate ip (days0,4,8,12;1800mg/kg),2)Iron-adequate. The Protocol was approved by the Committee on Experimental Animal Use and Care-UNS. In vitro studies: SH-SY5Y cells were treated with FAC30µM/72hs. Immunohistochemistry of mice brain and immunocytochemistry of cells was made for DMT1 and ZIP14. Perl`s staining. Results: Brain: In control mice, DMT1 expression was observed in striatum, hippocampus and cerebellum. In iron-overload, DMT1 expression in striatum, hippocampus and cerebellum was slight respect to control. In cerebellum, DMT1 was strongly expressed in granule cells, with slight immunoreactivity in Purkinje cells of control and iron-overloaded mice. ZIP14 was weakly expressed in striatum, hippocampus and cerebellum in control mice, while, in iron-overload, ZIP14 expression was strong. In cerebellum, ZIP14 was intensely expressed in granule cells and molecular layer control and iron-overloaded mice. Hemosiderin was absent in striatum, hippocampus and cerebellum of control mice, while in iron-overload abundant iron deposit was found. Neuronal cells: DMT1 immunoreactivity was lower in cells with FAC than that observed in control, while ZIP14 was higher. Conclusions: We concluded that iron excess accumulation that occurs in striatum, hippocampus and cerebellum could be the consequence of a high iron uptake produced by the increased ZIP14 expression, while DMT1 would not have a prominent role. In neurons, the increased ZIP14 and decreased DMT1 in iron-overload would suggest that iron importers regulations could be part of a mechanism that would involve cellular control.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/250857
Regulation of iron importers in regions of the central nervous system in iron accumulation models; Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 253-253
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/250857
identifier_str_mv Regulation of iron importers in regions of the central nervous system in iron accumulation models; Reunión conjunta SAIC SAI SAFIS 2018: LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad; Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 253-253
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.saic.org.ar/reuniones-anuales-previas
info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previas
info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación revista Medicina
publisher.none.fl_str_mv Fundación revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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