Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice

Autores
Giorgi, Gisela; Roque, Marta Elena
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is well known that the iron content of the body is tightly regulated. Iron excess induces adaptive changes that are differentially regulated in each tissue. The pancreas is particularly susceptible to iron-related disorders. We studied the expression and regulation of key iron proteins in the pancreas, duodenum and liver, using an animal model of iron overload (female CF1 mice injected i.p. with iron saccharate, colloidal iron form). Divalent metal transporter 1, prohepcidin and ferritin (pancreas, duodenum, liver) were assessed by immunohistochemistry; divalent metal transporter 1 (pancreas, duodenum) by Western blot. In the iron overloaded mice, prohepcidin expression increased in islets of Langerhans and hepatocytes, and divalent metal transporter 1 expression decreased in cells of islets and in enterocytes. In the iron overloaded mice, ferritin expression decreased in islets of Langerhans and increased in acinar cells; hemosiderin was localized in connective tissue cells. The inverse relationship between divalent metal transporter 1 and prohepcidin may indicate a negative regulation by hepcidin, and hence reduction of iron stores in islets of Langerhans. Our data showed that in iron overloaded mice model, induced by colloidal iron form, a coordinated expression of key iron proteins in the pancreas, duodenum and liver may occur. Further research will be necessary to determine the adaptive responses induced by iron in the pancreas.
Fil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Roque, Marta Elena. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Materia
Divalent Metal Transporter 1
Prohepcidin
Ferritin
Iron Overload
Pancreas
Mice
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35691
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/35691
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in miceGiorgi, GiselaRoque, Marta ElenaDivalent Metal Transporter 1ProhepcidinFerritinIron OverloadPancreasMicehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It is well known that the iron content of the body is tightly regulated. Iron excess induces adaptive changes that are differentially regulated in each tissue. The pancreas is particularly susceptible to iron-related disorders. We studied the expression and regulation of key iron proteins in the pancreas, duodenum and liver, using an animal model of iron overload (female CF1 mice injected i.p. with iron saccharate, colloidal iron form). Divalent metal transporter 1, prohepcidin and ferritin (pancreas, duodenum, liver) were assessed by immunohistochemistry; divalent metal transporter 1 (pancreas, duodenum) by Western blot. In the iron overloaded mice, prohepcidin expression increased in islets of Langerhans and hepatocytes, and divalent metal transporter 1 expression decreased in cells of islets and in enterocytes. In the iron overloaded mice, ferritin expression decreased in islets of Langerhans and increased in acinar cells; hemosiderin was localized in connective tissue cells. The inverse relationship between divalent metal transporter 1 and prohepcidin may indicate a negative regulation by hepcidin, and hence reduction of iron stores in islets of Langerhans. Our data showed that in iron overloaded mice model, induced by colloidal iron form, a coordinated expression of key iron proteins in the pancreas, duodenum and liver may occur. Further research will be necessary to determine the adaptive responses induced by iron in the pancreas.Fil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Roque, Marta Elena. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaJena Gustav Fischer Verlag2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35691Giorgi, Gisela; Roque, Marta Elena; Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice; Jena Gustav Fischer Verlag; Acta Histochemica; 116; 2; 6-2014; 354-3620065-1281CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.acthis.2013.08.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0065128113001645info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:43:56Zoai:ri.conicet.gov.ar:11336/35691instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:43:57.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
title Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
spellingShingle Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
Giorgi, Gisela
Divalent Metal Transporter 1
Prohepcidin
Ferritin
Iron Overload
Pancreas
Mice
title_short Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
title_full Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
title_fullStr Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
title_full_unstemmed Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
title_sort Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice
dc.creator.none.fl_str_mv Giorgi, Gisela
Roque, Marta Elena
author Giorgi, Gisela
author_facet Giorgi, Gisela
Roque, Marta Elena
author_role author
author2 Roque, Marta Elena
author2_role author
dc.subject.none.fl_str_mv Divalent Metal Transporter 1
Prohepcidin
Ferritin
Iron Overload
Pancreas
Mice
topic Divalent Metal Transporter 1
Prohepcidin
Ferritin
Iron Overload
Pancreas
Mice
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It is well known that the iron content of the body is tightly regulated. Iron excess induces adaptive changes that are differentially regulated in each tissue. The pancreas is particularly susceptible to iron-related disorders. We studied the expression and regulation of key iron proteins in the pancreas, duodenum and liver, using an animal model of iron overload (female CF1 mice injected i.p. with iron saccharate, colloidal iron form). Divalent metal transporter 1, prohepcidin and ferritin (pancreas, duodenum, liver) were assessed by immunohistochemistry; divalent metal transporter 1 (pancreas, duodenum) by Western blot. In the iron overloaded mice, prohepcidin expression increased in islets of Langerhans and hepatocytes, and divalent metal transporter 1 expression decreased in cells of islets and in enterocytes. In the iron overloaded mice, ferritin expression decreased in islets of Langerhans and increased in acinar cells; hemosiderin was localized in connective tissue cells. The inverse relationship between divalent metal transporter 1 and prohepcidin may indicate a negative regulation by hepcidin, and hence reduction of iron stores in islets of Langerhans. Our data showed that in iron overloaded mice model, induced by colloidal iron form, a coordinated expression of key iron proteins in the pancreas, duodenum and liver may occur. Further research will be necessary to determine the adaptive responses induced by iron in the pancreas.
Fil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Roque, Marta Elena. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description It is well known that the iron content of the body is tightly regulated. Iron excess induces adaptive changes that are differentially regulated in each tissue. The pancreas is particularly susceptible to iron-related disorders. We studied the expression and regulation of key iron proteins in the pancreas, duodenum and liver, using an animal model of iron overload (female CF1 mice injected i.p. with iron saccharate, colloidal iron form). Divalent metal transporter 1, prohepcidin and ferritin (pancreas, duodenum, liver) were assessed by immunohistochemistry; divalent metal transporter 1 (pancreas, duodenum) by Western blot. In the iron overloaded mice, prohepcidin expression increased in islets of Langerhans and hepatocytes, and divalent metal transporter 1 expression decreased in cells of islets and in enterocytes. In the iron overloaded mice, ferritin expression decreased in islets of Langerhans and increased in acinar cells; hemosiderin was localized in connective tissue cells. The inverse relationship between divalent metal transporter 1 and prohepcidin may indicate a negative regulation by hepcidin, and hence reduction of iron stores in islets of Langerhans. Our data showed that in iron overloaded mice model, induced by colloidal iron form, a coordinated expression of key iron proteins in the pancreas, duodenum and liver may occur. Further research will be necessary to determine the adaptive responses induced by iron in the pancreas.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35691
Giorgi, Gisela; Roque, Marta Elena; Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice; Jena Gustav Fischer Verlag; Acta Histochemica; 116; 2; 6-2014; 354-362
0065-1281
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35691
identifier_str_mv Giorgi, Gisela; Roque, Marta Elena; Iron overload induces changes of pancreatic and duodenal divalent metal transporter 1 and prohepcidin expression in mice; Jena Gustav Fischer Verlag; Acta Histochemica; 116; 2; 6-2014; 354-362
0065-1281
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.acthis.2013.08.013
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0065128113001645
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Jena Gustav Fischer Verlag
publisher.none.fl_str_mv Jena Gustav Fischer Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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